A Novel Dual-Mode Biosensing Platform Based on Au@luminol and CdSe QDs for Detection of Trace Heavy Metal Ions in PM2.5

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 278, P. 117366 - 117366

Published: March 10, 2025

Language: Английский

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Wavelength-Resolved Magnetic Multiplex Biosensor for Simultaneous and Ultrasensitive Detection of Pneumonia Pathogens via Catalytic Hairpin Assembly Strategy with Luminescent Iridium Complexes

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Pneumonia is a prevalent acute respiratory infection and major cause of mortality hospitalization, the urgent demand for rapid, direct, highly accurate diagnostic method capable detecting both Streptococcus pneumoniae (S. pneumoniae) Klebsiella (K. arises from their prominent roles as primary pathogens responsible pneumonia. Herein, two luminescent iridium complexes with nonoverlapping photoluminescence spectra, iridium(III)-bis [4,6-(difluorophenyl)-pyridinato-N,C2′] picolinate (abbreviated Ir–B) bis (2-(3,5- dimethylphenyl) quinoline-C2,N′) (acetylacetonato) iridium(III)) Ir-R), were unprecedently proposed to construct novel wavelength-resolved magnetic multiplex biosensor simultaneous detection S. K. based on catalytic hairpin assembly (CHA) signal amplification strategy combined dye-doped silica nanoparticles. Notably, not only exhibits broad linear range 50 pM 10 nM but also demonstrates excellent recovery rates (96.1–99.3%) (94.8–101.5%) in real clinical samples, corresponding relative standard deviation (RSD) values ranging 2.57 3.15% 1.45 3.17% pneumoniae. These favorable experimental outcomes undoubtedly offer promising approach multiple future.

Language: Английский

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Multiple-Signal Amplification Strategy to Fabricate an Ultrasensitive Electrochemiluminescence Magnetic Immunosensor for Detecting Biomarkers of Alzheimer’s Disease via Iridium-Based Self-Enhancing Nanoemitters

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Alzheimer's disease (AD) is characterized by progressive memory loss and cognitive decline, significantly impairing the daily life of elderly individuals. The low abundance blood-based biomarkers in AD necessitates higher analytical technique requirements. Herein, one novel iridium-based ECL self-enhanced nanoemitter (TPrA@Ir-SiO2) was unprecedentedly reported, it further used to construct an ultrasensitive magnetic immunosensor a multiple-signal amplification strategy unequally sensitively accurately detect biomarker (P-tau181) this work. initial signal accomplished via incorporating new efficient luminophore named Ir(mdq)2(acac) corresponding coreactant into silica nanoparticles successfully obtain TPrA@Ir-SiO2. In addition, specific high-affinity interactions between streptavidin biotin were subsequently employed facilitate amplification. Based on advantages itself streptavidin, proposed work exhibited remarkable sensitivity, covering wide linear range from 0.1 pg/mL μg/mL, achieved ultralow limit detection 68.58 fg/mL (S/N = 3), also outstanding recovery (98-104%) RSD (1.92-4.86%) serum samples spiking method. These results undoubtedly demonstrate potential nanoemitters combined with synergistic bearing streptavidin-biotin detecting biomarkers, providing accurate reliable solutions for early diagnosis monitoring AD, which would open avenue effectively reduce burden patients' families society future.

Language: Английский

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Low-Frequency Optical Signal Enhancement Device Combines CRISPR-Based Assay for Portable S. Pneumoniae Detection

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Early detection and treatment of Streptococcus pneumoniae (SPN) is crucial for patients. However, since nucleic acid testing relies on large-scale equipment specialized operators, challenges remain accurate, fast, low-cost SPN detection. Here, we present a point-of-care (POCT) device rapid accurate based low-frequency optical signal enhancement cluster regularly interspaced short palindromic repeats (CRISPR). The spotlight tube enables the fluorescence signal, while combination an artificial intelligence-assisted autoexposure algorithm homomorphic filtering image processing method improves signal-to-noise ratio image, thus realizing highly sensitive Nucleic identification performed using CRISPR-based crRNAs, fluorescent probes were constructed against IytA gene SPN. And they showed high specificity sensitivity gene. This demonstrated excellent in detecting developed strategy. threshold reached 0.1 fM, single time was only 40 min. Specificity validated clinical samples, test 100% agreement with quantitative polymerase chain reaction results from samples. provides device, which, novel DNA probe SPN, indicator option POCT

Language: Английский

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Self-Enhanced Electrochemiluminesence of Dye-Doped Polymer Dots for Coreactant-Free Visualized Detection of Iodide Ions

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(44), P. 17824 - 17830

Published: Oct. 22, 2024

The monitoring of radioactive iodide levels is great significance in environmental science and cancer radiotherapy. In this work, a high-throughput, radiation-resistant, visualized electrochemiluminescence (ECL) strategy was developed for detection ions. Herein, the hydrophobic ruthenium derivative (Ru(bpy)

Language: Английский

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