A critical review: Advanced electrochemical analysis based on nanoscale scanning electrochemical microscopy

Sensors and Actuators Reports, Journal Year: 2024, Volume and Issue: 8, P. 100243 - 100243

Published: Sept. 6, 2024

Language: Английский

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Super-Resolved Mapping of Electrochemical Reactivity in Single 3D Catalysts

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Crystals with three-dimensional (3D) stereoscopic structures, characterized by diverse shapes, crystallographic planes, and morphologies, represent a significant advancement in catalysis. Differentiating quantifying the catalytic activity of specific surface facets sites at single-particle level is essential for understanding predicting performance. This study employs super-resolution radial fluctuations electrogenerated chemiluminescence microscopy (SRRF-ECLM) to achieve high-resolution mapping electrocatalytic on individual 3D Cu2O crystals, including cubic, octahedral, truncated octahedral structures. With spatial resolution below 100 nm, SRRF-ECLM precisely delineates contours enabling detailed analysis distribution across distinct interfaces. By quantitatively measuring ECL emission intensities from different planes joint interfaces, we constructed distributions, offering an intuitive comprehensive perspective single-catalyst activity. approach advances electrochemical provides valuable insights designing more efficient catalysts energy conversion chemical synthesis applications.

Language: Английский

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Nanoscale Hydrophobicity of Transport Barriers in the Nuclear Pore Complex as Compared with the Liquid/Liquid Interface by Scanning Electrochemical Microscopy

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

The nuclear pore complex (NPC) is the proteinous nanopore that solely regulates molecular transport between nucleus and cytoplasm of a eukaryotic cell. Hypothetically, NPC utilizes hydrophobic barriers based on repeats phenylalanine–glycine (FG) units to selectively efficiently macromolecules. Herein, we quantitatively assess hydrophobicity confined in by applying scanning electrochemical microscopy (SECM). hypothesis deduced from studies isolated FG-rich nucleoporins supported investigating authentic for first time. Specifically, employ n neurotoxic glycine–arginine dipeptide, GRn, as probes engage interactions with NPC. We apply ion-transfer voltammetry at micropipet-supported interface aqueous organic electrolyte solutions confirm larger GRn among = 5–25 more hydrophobic, expected theoretically. micropipet also serves tip transient SECM demonstrate interacts strongly which supports barriers. Kinetically, stays longer clog nanopore, thereby expressing neurotoxicity. Significantly, this work implies efficient safe import genetic therapeutics requires an optimum balance strong association fast dissociation Interestingly, represents unexplored utility liquid/liquid interfaces models protein condensates liquid–liquid phase separation exemplified nanoscale

Language: Английский

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Scanning Electrochemical Microscopy: An Evolving Toolbox for Revealing the Chemistry within Electrochemical Processes

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Language: Английский

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Nanoscale interactions of arginine-containing dipeptide repeats with nuclear pore complexes as measured by transient scanning electrochemical microscopy

Chemical Science, Journal Year: 2024, Volume and Issue: 15(38), P. 15639 - 15646

Published: Jan. 1, 2024

The nuclear pore complex (NPC) plays imperative biological and biomedical roles as the sole gateway for molecular transport between cytoplasm nucleus of eukaryotic cells. proteinous nanopore, however, can be blocked by arginine-containing polydipeptide repeats (DPRs) proteins resulting from disordered C9orf72 gene a potential cause serious neurological diseases. Herein, we report new application transient scanning electrochemical microscopy (SECM) to quantitatively characterize DPR-NPC interactions first time. Twenty neurotoxic glycine-arginine proline-arginine in NPC are quantified match number phenylalanine-glycine (FG) units hydrophobic barriers nanopore. 1 : stoichiometry supports hypothesis that guanidinium residue DPR molecule engages cation-π with aromatic an FG unit. Cation-π interactions, too weak account measured free energy transfer water into NPC. is thermodynamically favorable receptors, which attributed hypothesized generally NPC-mediated macromolecular transport. Kinetically, DPRs trapped much longer than physiological thereby blocking Significantly, novel mechanism toxicity implies efficient safe import genetic therapeutics requires strong association fast dissociation Moreover, this work demonstrates unexplored power SECM determine thermodynamics kinetics membrane-molecule interactions.

Language: Английский

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Exploring Scanning Electrochemical Probe Microscopy in Single-Entity Analysis in Biology: Past, Present, and Future

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 271, P. 117060 - 117060

Published: Dec. 16, 2024

Language: Английский

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