Ultrasensitive Detection of FEN1 Activity for Cancer Diagnosis Using a CRISPR/Cas13a‐Based Triple Cascade Amplification System DOI

Xia Cheng,

Wenchen Zhao,

Daixi Chen

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 29, 2024

Flap endonuclease 1 (FEN1) is closely associated with tumor progression and proliferation, making it a promising biomarker for cancer diagnosis. However, developing sensitive, reliable, user-friendly method quantitative FEN1 detection remains technically challenging. In this study, an ultrasensitive biosensor established using target-induced cleavage-ligation-transcription-activation cascade strategy (LTACas13a) to enhance the cleavage ability of CRISPR/Cas13a. The LTACas13a has shown excellent performance in screening inhibitors detecting endogenous activity living cells, as well clinical biological samples such human serum tissue samples. Additionally, universal dumbbell probe derived from FEN1, multiplex developed various DNA glycosylases, including formamidopyrimidine glycosylase, uracil alkyl adenine glycosylase. This straightforward approach provides reliable effective diagnostic tool early-stage offers significant opportunities biosensing related drug discovery.

Language: Английский

Efficient SERS Substrate HOF@Au in Conjunction with DNAzyme-Mediated DNA Walker as a Signal Amplifier for the Ultrasensitive Detection of Lomefloxacin DOI

Runzi Zhang,

Shunbi Xie, Xiaoyu Yang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Lomefloxacin (LOM) is an efficacious antibiotic that might lead to liver damage and other adverse reactions if consumed over extended period. Hence, this study proposed a DNAzyme-mediated DNA walker as signal amplifier in combination with substantial quantity of Au nanoparticle (NP)-loaded hydrogen-bonded organic frameworks (HOFs) surface-enhanced Raman scattering (SERS) substrate accomplish the highly sensitive detection LOM food. Crucially, due their high specific surface area strong adsorption capacity, HOFs act ideal for situ growth large amount NPs, which exhibit robust uniform SERS enhancement. Once target was presented, DNAzyme activated start produce single abundance, further triggered catalytic hairpin assembly (CHA) cycle. In addition, multi-interstitial core-shell structure Au@Ag@4-nitrobenzenethiol (4-NTP)@Au@Hp3 produced more "hot spots" significantly amplified local electromagnetic field, HOF@Au synergistically strengthening 4-NTP. Using principle, sensor achieved ultrasensitive through "signal on" strategy, limit low 4.62 × 10-14 mol/L. This strategy explored meaningful innovative path design novel biosensor selective antibiotics

Language: Английский

Citations

2
DNA Nanotechnology-based strategies for gastric cancer diagnosis and therapy DOI Creative Commons
Congcong Li, Tongyang Xu, Guangjin Hou

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 30, P. 101459 - 101459

Published: Jan. 5, 2025

Gastric cancer (GC) is a formidable adversary in the field of oncology. The low early diagnosis rate GC results overall survival rate. Therefore, accurate and effective treatment are key to reduce mortality GC. With advent nanotechnology, researchers continue explore new possibilities for treatment. One such breakthrough application DNA nanotechnology. In this paper, exciting nanomaterials discussed depth. Firstly, biomarkers related diagnostic strategies nanotechnology summarized. Second, latest research progress targeted therapy Finally, challenges opportunities clinical prospected.

Language: Английский

Citations

0
Single-cell immunoassay of breast cancers based on their exosomal receptor expression profiles DOI
Xin Wei Wang, Jiaqi Wang, Shuping Xu

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159904 - 159904

Published: Jan. 1, 2025

Language: Английский

Citations

0
Multivalent Aptamer-Linked Tetrahedron DNA Assisted Catalytic Hairpin Assembly for Accurate Sers Assay of Cancer-Derived Exosomes in Clinical Blood DOI
Jingjing Zhang,

Chenlong Yan,

Lijie Xie

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Multivalent aptamer-linked tetrahedron DNA assisted catalytic hairpin assembly for accurate SERS assay of cancer-derived exosomes in clinical blood DOI
Jingjing Zhang,

Chenlong Yan,

Lijie Xie

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 282, P. 117497 - 117497

Published: April 18, 2025

Language: Английский

Citations

0
Ultrasensitive Detection of FEN1 Activity for Cancer Diagnosis Using a CRISPR/Cas13a‐Based Triple Cascade Amplification System DOI

Xia Cheng,

Wenchen Zhao,

Daixi Chen

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 29, 2024

Flap endonuclease 1 (FEN1) is closely associated with tumor progression and proliferation, making it a promising biomarker for cancer diagnosis. However, developing sensitive, reliable, user-friendly method quantitative FEN1 detection remains technically challenging. In this study, an ultrasensitive biosensor established using target-induced cleavage-ligation-transcription-activation cascade strategy (LTACas13a) to enhance the cleavage ability of CRISPR/Cas13a. The LTACas13a has shown excellent performance in screening inhibitors detecting endogenous activity living cells, as well clinical biological samples such human serum tissue samples. Additionally, universal dumbbell probe derived from FEN1, multiplex developed various DNA glycosylases, including formamidopyrimidine glycosylase, uracil alkyl adenine glycosylase. This straightforward approach provides reliable effective diagnostic tool early-stage offers significant opportunities biosensing related drug discovery.

Language: Английский

Citations

0