Efficient SERS Substrate HOF@Au in Conjunction with DNAzyme-Mediated DNA Walker as a Signal Amplifier for the Ultrasensitive Detection of Lomefloxacin
Runzi Zhang,
No information about this author
Shunbi Xie,
No information about this author
Xiaoyu Yang
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et al.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 2, 2025
Lomefloxacin
(LOM)
is
an
efficacious
antibiotic
that
might
lead
to
liver
damage
and
other
adverse
reactions
if
consumed
over
extended
period.
Hence,
this
study
proposed
a
DNAzyme-mediated
DNA
walker
as
signal
amplifier
in
combination
with
substantial
quantity
of
Au
nanoparticle
(NP)-loaded
hydrogen-bonded
organic
frameworks
(HOFs)
surface-enhanced
Raman
scattering
(SERS)
substrate
accomplish
the
highly
sensitive
detection
LOM
food.
Crucially,
due
their
high
specific
surface
area
strong
adsorption
capacity,
HOFs
act
ideal
for
situ
growth
large
amount
NPs,
which
exhibit
robust
uniform
SERS
enhancement.
Once
target
was
presented,
DNAzyme
activated
start
produce
single
abundance,
further
triggered
catalytic
hairpin
assembly
(CHA)
cycle.
In
addition,
multi-interstitial
core-shell
structure
Au@Ag@4-nitrobenzenethiol
(4-NTP)@Au@Hp3
produced
more
"hot
spots"
significantly
amplified
local
electromagnetic
field,
HOF@Au
synergistically
strengthening
4-NTP.
Using
principle,
sensor
achieved
ultrasensitive
through
"signal
on"
strategy,
limit
low
4.62
×
10-14
mol/L.
This
strategy
explored
meaningful
innovative
path
design
novel
biosensor
selective
antibiotics
Language: Английский
DNA Nanotechnology-based strategies for gastric cancer diagnosis and therapy
Materials Today Bio,
Journal Year:
2025,
Volume and Issue:
30, P. 101459 - 101459
Published: Jan. 5, 2025
Gastric
cancer
(GC)
is
a
formidable
adversary
in
the
field
of
oncology.
The
low
early
diagnosis
rate
GC
results
overall
survival
rate.
Therefore,
accurate
and
effective
treatment
are
key
to
reduce
mortality
GC.
With
advent
nanotechnology,
researchers
continue
explore
new
possibilities
for
treatment.
One
such
breakthrough
application
DNA
nanotechnology.
In
this
paper,
exciting
nanomaterials
discussed
depth.
Firstly,
biomarkers
related
diagnostic
strategies
nanotechnology
summarized.
Second,
latest
research
progress
targeted
therapy
Finally,
challenges
opportunities
clinical
prospected.
Language: Английский
Single-cell immunoassay of breast cancers based on their exosomal receptor expression profiles
Chemical Engineering Journal,
Journal Year:
2025,
Volume and Issue:
unknown, P. 159904 - 159904
Published: Jan. 1, 2025
Language: Английский
Multivalent Aptamer-Linked Tetrahedron DNA Assisted Catalytic Hairpin Assembly for Accurate Sers Assay of Cancer-Derived Exosomes in Clinical Blood
Jingjing Zhang,
No information about this author
Chenlong Yan,
No information about this author
Lijie Xie
No information about this author
et al.
Published: Jan. 1, 2025
Language: Английский
Multivalent aptamer-linked tetrahedron DNA assisted catalytic hairpin assembly for accurate SERS assay of cancer-derived exosomes in clinical blood
Jingjing Zhang,
No information about this author
Chenlong Yan,
No information about this author
Lijie Xie
No information about this author
et al.
Biosensors and Bioelectronics,
Journal Year:
2025,
Volume and Issue:
282, P. 117497 - 117497
Published: April 18, 2025
Language: Английский
Ultrasensitive Detection of FEN1 Activity for Cancer Diagnosis Using a CRISPR/Cas13a‐Based Triple Cascade Amplification System
Xia Cheng,
No information about this author
Wenchen Zhao,
No information about this author
Daixi Chen
No information about this author
et al.
Advanced Healthcare Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 29, 2024
Flap
endonuclease
1
(FEN1)
is
closely
associated
with
tumor
progression
and
proliferation,
making
it
a
promising
biomarker
for
cancer
diagnosis.
However,
developing
sensitive,
reliable,
user-friendly
method
quantitative
FEN1
detection
remains
technically
challenging.
In
this
study,
an
ultrasensitive
biosensor
established
using
target-induced
cleavage-ligation-transcription-activation
cascade
strategy
(LTACas13a)
to
enhance
the
cleavage
ability
of
CRISPR/Cas13a.
The
LTACas13a
has
shown
excellent
performance
in
screening
inhibitors
detecting
endogenous
activity
living
cells,
as
well
clinical
biological
samples
such
human
serum
tissue
samples.
Additionally,
universal
dumbbell
probe
derived
from
FEN1,
multiplex
developed
various
DNA
glycosylases,
including
formamidopyrimidine
glycosylase,
uracil
alkyl
adenine
glycosylase.
This
straightforward
approach
provides
reliable
effective
diagnostic
tool
early-stage
offers
significant
opportunities
biosensing
related
drug
discovery.
Language: Английский