Polarity-Targeted Carbon Dots for Mitochondria and Lysosomes Imaging DOI

Mengzhe Zhao,

Meng-Han Lin,

Ge Guo

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 14, 2024

Normally, electrostatic-dependent mitochondria localization can cause a decrease/loss of mitochondrial membrane potential (MMP), leading to the corresponding abnormal behaviors. So, achieving subcellular organelle and imaging with as little interference on their physiological activity is significance for understanding cell activity. Herein, we discover demonstrate that "polarity" independently act novel kind target labeling at level. On this basis, lysosomes are precisely fluorescently imaged by two kinds polarity-targeted carbon dots (C-dots), respectively. The C-dots, named C-dots-1 C-dots-2, have almost identical size morphology well surface chemistry. subtle difference polarity property: both them amphiphilic, 1.54 0.95 log P values. Different from commonly used cationic-based probes, C-dots possess slightly negatively charged surfaces (ζ-potential values ∼ -2.5 -7.5 mV) conditions. Interestingly, C-dots-2 capacity highly selectively lysosomes, whether cancer cells or normal cells. Because targeting processes do not rely electrostatic attraction effects, MMP changed during processes. behaviors caused diminishing, example, autophagy phenomenon, be effectively avoided.

Language: Английский

Application of a near-infrared viscosity-responsive fluorescent probe for lysosomal targeting in fatty liver mice DOI

Junlei Hao,

Xiaoying Li,

Suntao Shi

et al.

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: 155, P. 108162 - 108162

Published: Jan. 13, 2025

Language: Английский

Citations

2
Crosstalk-free two-channel visualization and precise assessment of autophagy-regulated hepatic ischemia-reperfusion injury by a two-photon fluorescent probe DOI

Qing Chang,

Qi Wang,

Jingyu Zhang

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137402 - 137402

Published: Feb. 1, 2025

Language: Английский

Citations

0
pH-/Viscosity-Activatable NIR Fluorescent Probes via Acceptor Engineering of Hemicyanine Dyes for High-Contrast Bioimaging DOI

Da Feng,

Li Guo,

Yihan Zhao

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 15, 2025

Real-time monitoring of the lysosomal microenvironment using small-molecule probes is critical for understanding complex interactions between organelles and diseases associated with dysfunction. Most traditional fluorescent-dye-based lysosome rely on protonation nitrogen atom in morpholine unit to visualize by inhibiting photoinduced electron transfer (PET) effect. However, these often face selectivity issues within cellular microenvironment. For instance, classic hemicyanine dyes (HD) show nonspecific fluorescence responses liver due inappropriate pKa value, leading low imaging contrast risk false positives. Herein, a series novel pH/viscosity-activatable, lysosomal-targeted near-infrared (NIR) fluorescent were developed incorporating naphthalimide dye dyes. These exhibit no at physiological pH weak under acidic conditions (key 1), substantial activation triggered abnormal viscosity pathological tissues 2). Notably, NpCy-4 demonstrated superior signal-to-background ratio (SBR) proved effective real-time situ diagnosis acute gastritis.

Language: Английский

Citations

0
Near-Infrared Fluorescent Lysosomal Viscosity Probe with Strong Solid Fluorescence for Rapid Imaging of Rheumatoid Arthritis DOI
Siyu Jiang, Zhoupeng Zheng, Yijia Liu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Rheumatoid arthritis (RA) is a chronic disease of widespread concern worldwide, and there an urgent need to develop sensitive methods for the rapid detection RA. Previous studies have shown that RA closely related lysosomal dysfunction. Lysosomal viscosity important microenvironmental parameter reflecting state lysosomes, but due lack probes demonstrate correlation between RA, changes in during remain unclear. For this purpose, we report herein lysosome-targeted near-infrared fluorescent molecular rotor probe DSMP investigate This utilizes dicyanomethylene-4H-benzothiopyran as electron acceptor fluorophore piperazine unit donor targeting group lysosomes. In addition, shows strong solid fluorescence response can effectively target lysosomes detect live cells. Based on this, established mouse model using λ-carrageenan. Mice imaging show quickly image tissues exhibit signals significantly brighter than those normal joint tissues. indicates increase RA; therefore, serve indicator be effective tool research.

Language: Английский

Citations

0
Imaging of sulfhydryl fluctuations in mouse models of drug-induced liver injury via a novel near-infrared fluorescence probe DOI
Qiyu Chen,

Ruipeng Shen,

Lijie Mi

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: 339, P. 126289 - 126289

Published: April 24, 2025

Language: Английский

Citations

0
A lipid droplet-targeted dual responding fluorescent probe for peroxynitrite and viscosity and its application in monitoring hyperglycemia and bioimaging DOI

Yana Tao,

Yun Dong,

Zhongwu Zhou

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113813 - 113813

Published: May 1, 2025

Language: Английский

Citations

0
Simultaneously detecting O2•-, polarity and viscosity with different fluorescence signals in Amiodarone-induced liver injury DOI
Yijia Liu, Qianhua Li, Zhoupeng Zheng

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137948 - 137948

Published: May 1, 2025

Language: Английский

Citations

0
Fabricating a Mitochondrial Viscosity-Activated Fluorescent Probe for Highly Sensitive Imaging of Kidney Injury In Vivo DOI
Xiaojiao Zheng, Hongshuai Zhang, Yuan‐Yuan Wang

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137972 - 137972

Published: May 1, 2025

Language: Английский

Citations

0
Polarity-Targeted Carbon Dots for Mitochondria and Lysosomes Imaging DOI

Mengzhe Zhao,

Meng-Han Lin,

Ge Guo

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 14, 2024

Normally, electrostatic-dependent mitochondria localization can cause a decrease/loss of mitochondrial membrane potential (MMP), leading to the corresponding abnormal behaviors. So, achieving subcellular organelle and imaging with as little interference on their physiological activity is significance for understanding cell activity. Herein, we discover demonstrate that "polarity" independently act novel kind target labeling at level. On this basis, lysosomes are precisely fluorescently imaged by two kinds polarity-targeted carbon dots (C-dots), respectively. The C-dots, named C-dots-1 C-dots-2, have almost identical size morphology well surface chemistry. subtle difference polarity property: both them amphiphilic, 1.54 0.95 log P values. Different from commonly used cationic-based probes, C-dots possess slightly negatively charged surfaces (ζ-potential values ∼ -2.5 -7.5 mV) conditions. Interestingly, C-dots-2 capacity highly selectively lysosomes, whether cancer cells or normal cells. Because targeting processes do not rely electrostatic attraction effects, MMP changed during processes. behaviors caused diminishing, example, autophagy phenomenon, be effectively avoided.

Language: Английский

Citations

1