Endogenous Free-Electron-Involved Coreactant-Free Electrochemiluminescence from Nanoclusters and Its Immunoassay Application DOI
Yaojia Ai, Xuwen Gao, Xiaowen Xu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

All of the commercialized electrochemiluminescence (ECL) immunoassays are automatically conducted at +1.40 V (vs Ag/AgCl) in coreactant route. To alleviate exogenous effect coreactants and simplify operation procedures, herein, a sulfur-vacancy-involved free electron strategy is proposed to exploit Au nanoclusters (NCs) as anodic electrochemiluminophores perform coreactant-free immunoassay. The deficient coordination between sulfhydryl Met core might induce departure partial S atoms enable Met-capped AuNCs (Met-AuNCs) with electron-rich nature. nature tends endow Met-AuNCs unpaired endogenous electrons, which can directly combine holes for light emitting. Coreactant-free ECL around +0.86 consequently conveniently achieved by merely oxidizing anode. qualified determine human carcinoembryonic antigen from 10 5000 pg/mL limit detection 5 pg/mL. Electron paramagnetic resonance provides clear evidence that electrons within play an important role generation ECL. This promising designing nanoelectrochemiluminophores improved immunoassay performance.

Language: Английский

Endogenous Free-Electron-Involved Coreactant-Free Electrochemiluminescence from Nanoclusters and Its Immunoassay Application DOI
Yaojia Ai, Xuwen Gao, Xiaowen Xu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

All of the commercialized electrochemiluminescence (ECL) immunoassays are automatically conducted at +1.40 V (vs Ag/AgCl) in coreactant route. To alleviate exogenous effect coreactants and simplify operation procedures, herein, a sulfur-vacancy-involved free electron strategy is proposed to exploit Au nanoclusters (NCs) as anodic electrochemiluminophores perform coreactant-free immunoassay. The deficient coordination between sulfhydryl Met core might induce departure partial S atoms enable Met-capped AuNCs (Met-AuNCs) with electron-rich nature. nature tends endow Met-AuNCs unpaired endogenous electrons, which can directly combine holes for light emitting. Coreactant-free ECL around +0.86 consequently conveniently achieved by merely oxidizing anode. qualified determine human carcinoembryonic antigen from 10 5000 pg/mL limit detection 5 pg/mL. Electron paramagnetic resonance provides clear evidence that electrons within play an important role generation ECL. This promising designing nanoelectrochemiluminophores improved immunoassay performance.

Language: Английский

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