One-Pot Detection of miRNA by Dual Rolling Circle Amplification at Ambient Temperature with High Specificity and Sensitivity DOI Creative Commons
Wenhua Sun,

Kunling Hu,

Ziting Song

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(5), P. 317 - 317

Published: May 15, 2025

Rolling circle amplification (RCA) at ambient temperature is prone to false positive signals during nucleic acid detection, which makes it challenging establish an efficient RCA detection method. The are primarily caused by binding of non-target acids the circular single-stranded template, leading non-specific amplification. Here, we present method for miRNA 37 °C using two ssDNAs, each formed ligating intramolecularly nick (without any splint) in a secondary structure. specific target recognition realized utilizing low concentrations (0.1 nM) ssDNA1 (C1). A phosphorothioate modification G*AATTC on C1 generate primer extension self-generated rolling (PG-RCA). fragmented products used as primers following that serves signal ssDNA2 (C2). Notably, absence splints and concentration significantly inhibits binding, thus minimizing signals. high (10 C2 carry out linear (LRCA), highly specific. This strategy demonstrates good response 0.01-100 pM with limit 7.76 fM (miR-155). Moreover, can distinguish single-nucleotide mismatch miRNA, enabling rapid one-pot °C. Accordingly, this performs specificity sensitivity. approach suitable clinical serum sample analysis offers developing biosensors diagnostic tools.

Language: Английский

Efficient Al–H3NTB-MOG ECL Emitter with Self-Enhanced and AIECL Performance for Ultrasensitive Sensing of miRNA-141 Combined with a Y-Shaped Multiregion Dual-Drive DNA Walker DOI
Jiping Yang, Li Wang, Yifei Chen

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

In this work, using Al-H3NTB-MOG with self-enhanced and aggregation-induced electrochemiluminescence (AIECL) performance as an efficient emitter, a biosensor based on Y-shaped multiregion dual-drive DNA walker was constructed for the sensitive detection of miRNA-141. Notably, 4,4',4″-nitrilotribenzoic acid (H3NTB) selected luminescent ligand ECL property co-reactive tertiary amine in its structure. Al3+ served central ion to coordinate H3NTB form three-dimensional porous gel structure, which restricted internal rotation vibration benzene molecules exhibited excellent AIECL property. More interestingly, N-2-hydroxyethylpiperazine-N'-ethane-sulfonic (HEPES) chosen system buffer, could not only stabilize test environment but also play co-reaction compensation role compensate consumption groups process, then significantly resulting better stability response. Besides, dynamic signal amplification established synergistic effect rolling cycle (RCA) process ionic cleavage at both ends nanostructure assembled by catalytic hairpin self-assembly (CHA) reaction. Specifically, two long chains abundant recognition regions were formed RCA reaction walker, simultaneously walk along predesigned tracks shear specific sites from directions, effectively improving efficiency. that way, realized miRNA-141 10 aM 1 nM range limit low 6.48 aM.

Language: Английский

Citations

0
One-Pot Detection of miRNA by Dual Rolling Circle Amplification at Ambient Temperature with High Specificity and Sensitivity DOI Creative Commons
Wenhua Sun,

Kunling Hu,

Ziting Song

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(5), P. 317 - 317

Published: May 15, 2025

Rolling circle amplification (RCA) at ambient temperature is prone to false positive signals during nucleic acid detection, which makes it challenging establish an efficient RCA detection method. The are primarily caused by binding of non-target acids the circular single-stranded template, leading non-specific amplification. Here, we present method for miRNA 37 °C using two ssDNAs, each formed ligating intramolecularly nick (without any splint) in a secondary structure. specific target recognition realized utilizing low concentrations (0.1 nM) ssDNA1 (C1). A phosphorothioate modification G*AATTC on C1 generate primer extension self-generated rolling (PG-RCA). fragmented products used as primers following that serves signal ssDNA2 (C2). Notably, absence splints and concentration significantly inhibits binding, thus minimizing signals. high (10 C2 carry out linear (LRCA), highly specific. This strategy demonstrates good response 0.01-100 pM with limit 7.76 fM (miR-155). Moreover, can distinguish single-nucleotide mismatch miRNA, enabling rapid one-pot °C. Accordingly, this performs specificity sensitivity. approach suitable clinical serum sample analysis offers developing biosensors diagnostic tools.

Language: Английский

Citations

0