Novel Approaches in CRISPR/Cas12a-Based Sensing for HCC Diagnosis-A Review (2020-2025)

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2025, Volume and Issue: 262, P. 116878 - 116878

Published: April 8, 2025

Language: Английский

Arrest of CRISPR-Cas12a by Nonspecific Single-Stranded DNA for Biosensing

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

CRISPR-Cas technologies have emerged as powerful biosensing tools for the sensitive and specific detection of non-nucleic acid targets. However, existing strategies suffer from poor compatibility across diverse targets due to complicated engineering crRNA DNA activator required activity regulation. Herein, we report a novel straightforward strategy designing CRISPR-Cas12a-based biosensors that function by switching structures single-stranded (ss)DNA/CRISPR-Cas12a assembly activator/CRISPR-Cas12a complex in presence target bacterium. The begins with ssDNA made trans-acting RNA-cleaving DNAzyme (tRCD) an RNA/DNA chimeric substrate (RCS). has ability bind Cas12a nonspecifically, thus indeed blocking CRISPR-Cas12a activity. By exploiting recognition cleavage capacities tRCD RCS target, target-bound cleaved are released Cas12a, restoring This method been successfully applied (detection limit: 102 CFU/mL) Escherichia coli (E. coli, EC) Burkholderia gladioli (B. gladioli, BG). For blind testing 30 clinical urine samples, it exhibited 100% sensitivity specificity identifying E. coli-associated urinary tract infections (UTIs).

Language: Английский

A CRISPR-Cas12a-based regenerative surface plasmon resonance biosensor for cytomegalovirus detection

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 114013 - 114013

Published: May 1, 2025

Language: Английский