Bio-orthogonal Labeling of Chitin in Native Pathogenic Candida Species via the Chitin Scavenge Pathway DOI
Caroline Williams,

Bella R. Carnahan,

Stephen N. Hyland

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

The fungal cell wall is essential for the integrity of cell, providing strength and shape, as well protection against environmental stimuli. For pathogenic fungi, also initial point contact with host. Specific features such hypha tails smaller glycan components modulate a wide range interactions immune defenses. Here, bio-orthogonal labeling method utilizing N-acetyl-glucosamine (NAG) probes developed to fluorescently label native, yeast via chitin scavenging pathway. A panel NAG was assembled, synthesized, characterized ability in yeast. Enzymatic data show that native biosynthetic enzyme, Hxk1, promiscuous, permitting biopolymer. This validated development mass spectrometry protocols. When compared current available systems chitin, do not affect interrupt growth. Furthermore, enabled multiple "click" platforms across Candida species including albicans tropicalis. Budding filamentous hyphal states were observed. results indicate probes' utility vivo study morphological, switch, visualization growth patterns. Thus, use these strains ideal variety future applications strain specific antifungals, diagnostic tools, immunomodulators.

Language: Английский

Bio-orthogonal Labeling of Chitin in Native Pathogenic Candida Species via the Chitin Scavenge Pathway DOI
Caroline Williams,

Bella R. Carnahan,

Stephen N. Hyland

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

The fungal cell wall is essential for the integrity of cell, providing strength and shape, as well protection against environmental stimuli. For pathogenic fungi, also initial point contact with host. Specific features such hypha tails smaller glycan components modulate a wide range interactions immune defenses. Here, bio-orthogonal labeling method utilizing N-acetyl-glucosamine (NAG) probes developed to fluorescently label native, yeast via chitin scavenging pathway. A panel NAG was assembled, synthesized, characterized ability in yeast. Enzymatic data show that native biosynthetic enzyme, Hxk1, promiscuous, permitting biopolymer. This validated development mass spectrometry protocols. When compared current available systems chitin, do not affect interrupt growth. Furthermore, enabled multiple "click" platforms across Candida species including albicans tropicalis. Budding filamentous hyphal states were observed. results indicate probes' utility vivo study morphological, switch, visualization growth patterns. Thus, use these strains ideal variety future applications strain specific antifungals, diagnostic tools, immunomodulators.

Language: Английский

Citations

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