Harnessing the power of ginger leaf polysaccharide: A potential strategy to combat Aβ-induced toxicity through the Wnt/β-catenin pathway

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 303, P. 140692 - 140692

Published: Feb. 4, 2025

Language: Английский

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Facially Amphiphilic Cholate-Conjugated Polymers for Regulating Insulin Fibrillation

Bioconjugate Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

To understand the influence of facially amphiphilic polymers (FAPs) on insulin fibril (IF) inhibition, three different cholate-based FAPs [cationic (PFCAQA), anionic (PFCASF), and zwitterionic (PFCASB)] have been synthesized. Besides, two control [cholate sulfobetaine-pendant random copolymer PRCASB (without facial amphiphilicity) sulfobetaine-tethered homopolymer PSBMA cholate pendants)] are also prepared. Several biophysical experiments such as spectroscopic techniques [thioflavin T (ThT), Nile red (NR), tyrosine (Tyr) fluorescence assay], turbidity assay by ultraviolet-visible (UV-vis) spectroscopy, dynamic light scattering (DLS), circular dichroism (CD) study, microscopic investigation performed to investigate role antiamyloidogenic agents during fibrillation. Interestingly, PFCASB polymer behaves most efficacious agent. clarify interaction native (NI), an isothermal titration calorimetry (ITC) experiment is carried out. Tyr NR suggest important hydrophobic interactions, whereas ITC confirms significance electrostatic interactions in IF inhibitory process. A hemolytic test conducted toxicity caused efficacy prohibiting erythrocyte disruption IF. Overall, present work reveals impact cholic acid (CA)-based modulating aggregation process gives a new perspective for investigations protein aggregations.

Language: Английский

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RAFT‐engineered polymeric surfactant: Exploring the self‐assembly of homopolymers from poly(ethylene glycol) methyl ether methacrylate

Journal of Surfactants and Detergents, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Abstract Polymeric surfactants, valued for their ability to stabilize interfaces and tunable self‐assembled structures, find extensive applications in personal care, drug delivery, pharmaceuticals, industrial formulations. To develop an efficient polymeric surfactant, herein we investigate the synthesis characterization of side‐chain poly(ethylene glycol) ( PEG )‐based homopolymers PPEGMAx ), using reversible addition‐fragmentation chain transfer (RAFT) polymerization presence a hydrophobic tail‐functionalized agent (CTA), enabling precise control over molar mass narrow dispersity Đ ). Structural confirmation compositional analysis are performed 1 H nuclear magnetic resonance NMR) spectroscopy. The amphiphilic nature self‐assembly behavior polymers investigated through fluorescence spectroscopy, dynamic light scattering (DLS), atomic force microscopy (AFM). show critical aggregation concentrations range 27–63 μg/mL water, with sizes ranging from 40 80 nm. However, suitable hydrophobic/hydrophilic balance polymer structure is necessary potential as surfactants.

Language: Английский

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Mechanistic interaction studies on variform PCN-224 nanoparticles with transferrin and lysozyme

Journal of Molecular Structure, Journal Year: 2025, Volume and Issue: unknown, P. 142728 - 142728

Published: May 1, 2025

Language: Английский

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Insights into the Interactions Between Monolayer‐Protected Metal Nanoclusters and Amyloid‐β Fibrils

Advanced Theory and Simulations, Journal Year: 2025, Volume and Issue: unknown

Published: May 21, 2025

Abstract Monolayer‐protected atomically precise metal nanoclusters (MPCs) have attracted significant attention from the scientific community because of their unique structural and physicochemical properties. In addition to diverse applications in bioimaging biosensing, water‐soluble MPCs shown promise as nanotherapeutics. However, in‐depth knowledge how act therapeutic agents remained unexplored. this work, inhibition mechanism amyloid fibril growth by gold functionalized with positively charged ligands, 8‐MTA (mercaptooctyltrimethylammonium) polyphenol‐based ‐alkyl derivative epigallocatechin‐3‐gallate (EGCG) is investigated using extensive all‐atom molecular dynamics simulations. The simulations reveal that ligand‐coated small bind different external sides an stipulate its secondary structure content. Non‐covalent interactions such electrostatic, van der Waals, –, hydrogen bonding help various regions thereby exert conformational changes on peptides fibril. pertinent atomistic insights obtained work will spur design ligand‐functionalized nanodrugs can potentially inhibit connected Alzheimer's other neurodegenerative diseases.

Language: Английский

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From experimental studies to computational approaches: recent trends in designing novel therapeutics for amyloidogenesis

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

This review highlights the integration of various experimental and computational methods to control amyloid aggregation process. We believe that this article will help researchers develop novel therapeutic influence protein aggregation.

Language: Английский

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Differential effects of SARS-CoV-2 amyloidogenic segments on the aggregation and toxicity of human islet amyloid polypeptide within membrane environments

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 283, P. 137930 - 137930

Published: Nov. 21, 2024

Language: Английский

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