Networked Cluster Formation via Trigonal Lipid Modules for Augmented Ex Vivo NK Cell Priming DOI Open Access
Jaewon Park, Sungjun Kim, Ashok Kumar Jangid

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1556 - 1556

Published: Jan. 26, 2024

Current cytokine-based natural killer (NK) cell priming techniques have exhibited limitations such as the deactivation of biological signaling molecules and subsequent insufficient maturation population during mass cultivation processes. In this study, we developed an amphiphilic trigonal 1,2-distearoyl-sn-glycero-3-phosphorylethanolamine (DSPE) lipid-polyethylene glycol (PEG) material to assemble NK clusters via multiple hydrophobic lipid insertions into cellular membranes. Our conjugate-mediated ex vivo sufficiently augmented structural modulation clusters, facilitated diffusional signal exchanges, finally activated with clusters. Without any inhibition in exchanges intrinsic proliferative efficacy cells, effectively prime produced increased interferon-gamma, especially early culture periods. conclusion, present study demonstrates that our novel conjugates could serve a promising alternative for future production.

Language: Английский

Phenylboronic acid-functionalized biomaterials for improved cancer immunotherapy via sialic acid targeting DOI
Ashok Kumar Jangid, Kyobum Kim

Advances in Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 333, P. 103301 - 103301

Published: Sept. 6, 2024

Language: Английский

Citations

5
Amphiphilic Lipid-Peptide Engineered Placenta-Derived Mesenchymal Stem Cells for Liver Fibrosis Treatment DOI Creative Commons
Hee Won Park,

Dae Hyun Lee,

Sungjun Kim

et al.

Asian Journal of Pharmaceutical Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 101061 - 101061

Published: April 1, 2025

Language: Английский

Citations

0
Amphiphilic Polyaspartamide Derivatives with Cholesterol Introduction Enhanced Ex Vivo mRNA Transfection Efficiency to Natural Killer Cells DOI

David Koam,

Ha Yeon Park,

Dong Sun Kim

et al.

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Engineered natural killer (NK) cells eliminate cancer by overexpressing a chimeric antigen receptor, producing highly efficient and safe NK cell therapies. This study investigated the polyplex formulation for fusion protein GreenLantern-natural group 2D (NKG2D) mRNA to evaluate its ex vivo delivery efficacy into cells, wherein NKG2D on surface of recognized counterpart ligands cells. Amphiphilic polyaspartamide derivatives Chol-PAsp(DET/CHE) were prepared adding cyclohexylethylamine (CHE) diethylenetriamine (DET) in side chains cholesterol (Chol) at α-terminus enhance endosomal escapability optimize hydrophobicity. significantly improved NK-92mi explained increased stability cellular uptake mRNA. The NKG2D-overexpressing exhibited high anticancer against human colon without affecting viability fibroblasts. Therefore, could be promising carrier engineering

Language: Английский

Citations

0
Delivery of piperlongumine via hyaluronic acid/phenylboronic acid-mediated dual targetable polymersome for enhanced anticancer functionality against pancreatic tumor DOI
Ashok Kumar Jangid, Sung Jun Kim, Kyobum Kim

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 275, P. 133738 - 133738

Published: Aug. 1, 2024

Language: Английский

Citations

3
Phenylboronic Acid-Modified pH/Glucose Dual-Responsive Polymeric Micelles for Targeted Anticancer Drug Delivery DOI
Shushu Zhao, Fengjiao Chen,

Xianwu Chen

et al.

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

The tumor microenvironment is characterized by several hallmarks such as an acidic pH and high glucose levels in tissues increased expression of specific proteins and/or sugars on the surface cells. These unique tumors can be considered design multifunctional drug delivery nanosystems to improve efficiency therapy through targeted release tissue. In this study, phenylboronic acid-modified pH- glucose-responsive polymer micelles were designed for anticancer drugs. polymeric demonstrated prolonged pH/glucose-triggered enhanced cellular internalization B16F10 cells a receptor-mediated endocytosis pathway. micellar system could inhibit proliferation with IC50 values lower than those unmodified micelles. addition, markedly suppress cell migration, colony formation, invasion promote apoptosis cells, indicating good vitro. Therefore, nanocarrier provides potential platform therapy.

Language: Английский

Citations

3
Membrane-immobilized Gemcitabine for Cancer-Targetable NK Cell Surface Engineering DOI

Kyung Mu Noh,

Ashok Kumar Jangid, Jaewon Park

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(46), P. 12087 - 12102

Published: Jan. 1, 2024

Although natural killer (NK) cell-based adoptive cell transfer (ACT) has shown promise in cancer immunotherapy, its efficacy against solid tumors is limited the immunosuppressive tumor microenvironment (TME). Combinatorial therapies involving chemotherapeutic drugs such as gemcitabine (Gem) and NK cells have been developed to modulate TME; however, their clinical application constrained by low drug delivery efficiency significant off-target toxicity. In this study, we membrane-immobilized Gem conjugates (

Language: Английский

Citations

2
Amphiphilic Lipid Conjugate-mediated surface engineering of Placenta-Derived mesenchymal stem cells for alleviating liver damage and fibrosis DOI
Sungjun Kim,

Dae Hyun Lee,

Hee Won Park

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 503, P. 158313 - 158313

Published: Dec. 9, 2024

Language: Английский

Citations

2
Advancements of engineered live oncolytic biotherapeutics (microbe/virus/cells): Preclinical research and clinical progress DOI

Zhengzhuo Qi,

Junmou Gu,

Lihang Qu

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 375, P. 209 - 235

Published: Sept. 11, 2024

Language: Английский

Citations

0
Networked Cluster Formation via Trigonal Lipid Modules for Augmented Ex Vivo NK Cell Priming DOI Open Access
Jaewon Park, Sungjun Kim, Ashok Kumar Jangid

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1556 - 1556

Published: Jan. 26, 2024

Current cytokine-based natural killer (NK) cell priming techniques have exhibited limitations such as the deactivation of biological signaling molecules and subsequent insufficient maturation population during mass cultivation processes. In this study, we developed an amphiphilic trigonal 1,2-distearoyl-sn-glycero-3-phosphorylethanolamine (DSPE) lipid-polyethylene glycol (PEG) material to assemble NK clusters via multiple hydrophobic lipid insertions into cellular membranes. Our conjugate-mediated ex vivo sufficiently augmented structural modulation clusters, facilitated diffusional signal exchanges, finally activated with clusters. Without any inhibition in exchanges intrinsic proliferative efficacy cells, effectively prime produced increased interferon-gamma, especially early culture periods. conclusion, present study demonstrates that our novel conjugates could serve a promising alternative for future production.

Language: Английский

Citations

0