Ultrastructural viscoelastic behavior of collagen identified by AFM nano-dynamic mechanical analysis DOI Open Access
Meisam Asgari, Elahe Mirzarazi Dahagi, Hojatollah Vali

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 22, 2024

Abstract Soft tissues exhibit predominantly time-dependent mechanical behavior critical for their biological function in organs like the lungs and aorta, as they can deform stretch at varying rates depending on function. Collagen type I serves primary structural component these tissues. The viscoelastic characteristics of such tissues, stemming from diverse energy dissipation mechanisms across various length scales, remains poorly characterized nanoscale. Furthermore, prior experimental investigations have centered analyzing tissue responses largely attributed to interactions between cells fibers. Despite many studies viscoelasticity scaffolds single collagen fibrils, mechanics fibrils sub-fibrillar level remain understood. This pioneering study employs atomic force microscopy (AFM) nano-rheometry examine individual ultrastructural within distinct topographical zones, specifically focusing gap overlap regions. Our investigation has unveiled that obtained via in-vitro fibrillogenesis human placenta display a response replicates macroscale. Further, our findings suggest regions, likely variances molecular organization cross-linking modalities specific sites. results furnish unequivocal proof temporal dependence properties provides unique data be compared atomistic models, laying foundation refining precision macroscale models strive capture scales.

Language: Английский

Water and Collagen: A Mystery Yet to Unfold DOI Creative Commons
Guido Giannetti,

Fumiki Matsumura,

Federico Caporaletti

et al.

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Collagen is the most abundant protein in human body and plays an essential role determining mechanical properties of tissues. Both as a monomeric fibrous assemblies, collagen interacts with its surrounding molecules, particular water. Interestingly, while it well established that interaction water strongly influences molecular underlying mechanisms remain largely unknown. Here, we review research conducted over past 30 years on interplay between relevance for tissue properties. We discuss water-collagen relevant time- length scales, ranging from vital stabilizing characteristic triple helix structure to negative impact dehydration A better understanding will help unravel effect mutations defective production collagen-related diseases pinpoint key design features required synthesize collagen-based biomimetic tissues tailored

Language: Английский

Citations

0
Ultrastructural viscoelastic behavior of collagen identified by AFM nano-dynamic mechanical analysis DOI Open Access
Meisam Asgari, Elahe Mirzarazi Dahagi, Hojatollah Vali

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 22, 2024

Abstract Soft tissues exhibit predominantly time-dependent mechanical behavior critical for their biological function in organs like the lungs and aorta, as they can deform stretch at varying rates depending on function. Collagen type I serves primary structural component these tissues. The viscoelastic characteristics of such tissues, stemming from diverse energy dissipation mechanisms across various length scales, remains poorly characterized nanoscale. Furthermore, prior experimental investigations have centered analyzing tissue responses largely attributed to interactions between cells fibers. Despite many studies viscoelasticity scaffolds single collagen fibrils, mechanics fibrils sub-fibrillar level remain understood. This pioneering study employs atomic force microscopy (AFM) nano-rheometry examine individual ultrastructural within distinct topographical zones, specifically focusing gap overlap regions. Our investigation has unveiled that obtained via in-vitro fibrillogenesis human placenta display a response replicates macroscale. Further, our findings suggest regions, likely variances molecular organization cross-linking modalities specific sites. results furnish unequivocal proof temporal dependence properties provides unique data be compared atomistic models, laying foundation refining precision macroscale models strive capture scales.

Language: Английский

Citations

0