Preparation and Antibacterial Activity Evaluation of Daphnetin-Loaded Poloxamers/Polyvinylpyrrolidone Thermosensitive Hydrogels DOI

Junhong Fan,

Fengli An,

Shaohua Li

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

Antibiotic misuse and bacterial resistance are pressing issues threatening public health. Natural plant extracts with bactericidal properties offer potential alternatives to reduce or replace antibiotic use. This study aims develop a thermosensitive hydrogel containing daphnetin (DAP-TG) using poloxamers 407 (P407), polyvinylpyrrolidone (PVP), 188 (P188). We systematically evaluated the gel's antibacterial activity against Escherichia coli (E. coli) Staphylococcus aureus (S. aureus), as well its mechanisms. By examining gelation temperature time, degradation in vitro release performance of DAP-TG, we produced sustained-release DAP-TG rapid phase transition at (31.6 ± 0.1) °C. Its structure was characterized through Fourier transform infrared (FTIR) spectroscopy scanning electron microscopy (SEM). The results indicated that DAP formed presented 3D network spatial structure. biocompatibility explored hemolysis test cytotoxicity test. possessed excellent biocompatibility. efficacy E. S. assessed minimum inhibitory concentration (MIC), (MBC), growth curve, inhibition zone tests. Results showed exhibited both aureus, MIC values 1.28 0.32 mg/mL. mechanism preliminarily investigation cell content leakage, AKP membrane permeability, SEM, ROS production, biofilm activity. induced irreversible damage membranes resulting enhanced elevated levels, inhibited formation. Our indicates exhibits effective vitro, making it promising candidate for applications food pharmaceutical products.

Language: Английский

Preparation and Antibacterial Activity Evaluation of Daphnetin-Loaded Poloxamers/Polyvinylpyrrolidone Thermosensitive Hydrogels DOI

Junhong Fan,

Fengli An,

Shaohua Li

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

Antibiotic misuse and bacterial resistance are pressing issues threatening public health. Natural plant extracts with bactericidal properties offer potential alternatives to reduce or replace antibiotic use. This study aims develop a thermosensitive hydrogel containing daphnetin (DAP-TG) using poloxamers 407 (P407), polyvinylpyrrolidone (PVP), 188 (P188). We systematically evaluated the gel's antibacterial activity against Escherichia coli (E. coli) Staphylococcus aureus (S. aureus), as well its mechanisms. By examining gelation temperature time, degradation in vitro release performance of DAP-TG, we produced sustained-release DAP-TG rapid phase transition at (31.6 ± 0.1) °C. Its structure was characterized through Fourier transform infrared (FTIR) spectroscopy scanning electron microscopy (SEM). The results indicated that DAP formed presented 3D network spatial structure. biocompatibility explored hemolysis test cytotoxicity test. possessed excellent biocompatibility. efficacy E. S. assessed minimum inhibitory concentration (MIC), (MBC), growth curve, inhibition zone tests. Results showed exhibited both aureus, MIC values 1.28 0.32 mg/mL. mechanism preliminarily investigation cell content leakage, AKP membrane permeability, SEM, ROS production, biofilm activity. induced irreversible damage membranes resulting enhanced elevated levels, inhibited formation. Our indicates exhibits effective vitro, making it promising candidate for applications food pharmaceutical products.

Language: Английский

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