Nanoplastics Intensify the Inhibitory Effect of Di‐n‐octylo‐phthalate on Trypsin: Spectroscopic and Computer Simulation Analysis

Chemistry & Biodiversity, Journal Year: 2025, Volume and Issue: unknown

Published: May 30, 2025

ABSTRACT Given their frequent coexistence of plasticizers and nanoplastics, assessing combined toxicological impacts is essential. This study investigates the interactions between di‐n‐octyl phthalate (DNOP) trypsin (TRY) in presence polystyrene nanoplastics (PSNPs) at molecule levels. Molecular docking analysis demonstrates that DNOP could enter active site TRY. Spectral analyses indicate changed TRY's conformation residue microenvironment, which were exacerbated by PSNPs. Particle size transmission electron microscope show PSNPs adsorbed TRY, forming a protein corona. Enzyme activity experiments confirm inhibited with further enhancing this inhibitory effect. Specifically, bind to inducing conformational changes expose additional binding sites, while adsorbing increase local concentration promote interaction The findings provide novel insights when evaluating risks associated both phthalates.

Language: Английский

Evaluation of cross-linkers in the design of immobilized multi isomerase cascade for the preparation of rare sugars

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 287, P. 138592 - 138592

Published: Dec. 9, 2024

Language: Английский