Environmental Stability Determines the Cytotoxicity of Metal–Organic Frameworks to a Nitrogen-Fixing Bacterium Azotobacter vinelandii DOI
Ziqi Tang,

Chengzhuang Liang,

Qinmei Zhong

et al.

Chemical Research in Toxicology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

During widespread applications of metal–organic frameworks (MOFs), the environmental hazards and risks MOFs have aroused great concerns. In this study, we aimed to reveal importance stability on their toxicity. Two Zn-MOFs, namely, ZIF-8 with high aqueous Zn-BDC low stability, were compared directly in toxicological evaluations a nitrogen-fixing bacterium Azotobacter vinelandii. showed strong cytotoxicity at 100 mg/L higher, inducing growth inhibition, cell apoptosis, structural changes, oxidative damage, and, consequently, loss nitrogen fixation ability. contrast, was nearly nontoxic A. The transcriptome analysis that disturbed ribosome pathway lowered expression level nif cluster genes. On other hand, stress could regulate flagellar assembly, siderophore group nonribosomal peptide biosynthesis, bacterial chemotaxis, amino sugar nucleotide metabolism pathways promote Beyond that, toxicity Zn-MOFs vinelandii associated release Zn2+, but less toxic than mixtures starting materials. Overall, our results suggested determined through different molecular pathways. Designing stable is preferred due environment-friendly considerations.

Language: Английский

Absorptivity Is an Important Determinant in the Toxicity Difference between Aristolochic Acid I and Aristolochic Acid II DOI

Hong-Ching Kwok,

Hei-Tak Tse,

Kwan Wah Ng

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Inadvertent exposure to aristolochic acids (AAs) is causing chronic renal disease worldwide, with acid I (AA-I) identified as the primary toxic agent. This study employed chemical methods investigate mechanisms underlying nephrotoxicity and carcinogenicity of AA-I. Aristolochic II (AA-II), which has a structure similar that AA-I, was investigated same for comparison. Despite their structural similarities, findings from cultured human cells gut sac experiments showed AA-I absorbed more effectively than AA-II (∼3 times greater AA-II; p < 0.001). increased absorption, along previously observed higher activity reductive activation enzymes results in DNA damage oxidative stress, both are key factors AA-related toxicity. The patterns cell mortality (34.4 ± 2.3% vs 9.7 0.1% at 80 μM; 0.0001), adduct formation 0.001), stress levels relation concentrations indicate absorption rate significant contributor its toxicity also found be further enhanced by (natural) coexistence AA-II. Since differ only methoxy group, future research on reducing risks associated AA should focus strategies lower these compounds.

Language: Английский

Citations

0
Discrimination of poisonous and medicinal plants with similar appearance (Asarum heterotropoides vs. Cynanchum paniculatum) via a fusion method of E-nose, E-tongue, LC-HR-Q-TOF-MS/MS, and electrochemical fingerprint spectra DOI Creative Commons
Xinru Zhang, Yuehua Chen,

Jia‐Nuo Zhang

et al.

Frontiers in Chemistry, Journal Year: 2025, Volume and Issue: 13

Published: April 29, 2025

The similarity in appearance of poisonous and medicinal plants, such as Asarum heterotropoides (AH) Cynanchum paniculatum (CP), poses safety risks due to frequent confusion. Since AH contains toxic ingredients, the traditional methods olfactory gustatory identification cannot be used distinguish from CP. To differentiate them systematically, we proposed a novel strategy based on dual electronic sensors (DES) fingerprint spectra (DFS). DES included two intelligent sensors, namely E-nose E-tongue, which differentiated CP odor taste, respectively. DFS comprised chemical obtained through LC-HR-Q-TOF-MS/MS electrochemical derived Belousov-Zhabotinsky reaction, differentiating by their specific overall compositions, our knowledge, this was first time that E-nose, LC-HR-Q-TOF-MS/MS, reaction were combined identify With identified 25 major components 12 single run 140 s. Using bitterness astringency primary taste differences. Furthermore, 91 compounds 90 LC-HR-Q-TOF-MS/MS. Both shared nitrogenous compounds, volatile oils, organic acids, lignans. However, uniquely contained coumarins flavonoids, while steroidal saccharides. Notably, also possessed distinct components, specifically aristolactam I, aristolochic acid D, safrole. Based CP, thereby facilitating further distinction between these herbs. Through combination with principal component analysis (PCA) or orthogonal partial least squares-discriminant (OPLS-DA), accuracy method reached 100%. fusion strategy, odors, tastes, properties have been systematically analyzed.

Language: Английский

Citations

0
Catalpol from Rehmannia glutinosa Targets Nrf2/NF-κB Signaling Pathway to Improve Renal Anemia and Fibrosis DOI

Zhi-Hui Liu,

Qingyang Xu,

Yu Wang

et al.

The American Journal of Chinese Medicine, Journal Year: 2024, Volume and Issue: 52(05), P. 1451 - 1485

Published: Jan. 1, 2024

is widely recognized as a prominent medicinal herb employed by practitioners across various generations for the purpose of fortifying kidney yin. Within

Language: Английский

Citations

2
Fabrication of a Simple and Efficient HPLC Reduction Column for Online Conversion of Aristolochic Acids to Aristolactams Prior to Sensitive Fluorescence Detection DOI

Man-Lung Chin,

Chun-Kit Au, Chi‐Kong Chan

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(33), P. 12365 - 12372

Published: Aug. 11, 2023

Aristolochic acids (AAs) are nephrotoxic and carcinogenic nitrophenanthrene carboxylic produced naturally by plants from the Aristolochia Asarum genera, which have been used extensively as herbal medicines. In addition to consuming AA-containing medicinal products, there is emerging evidence that humans also exposed AA through environment. 2022, World Health Organization (WHO) called for global action remove exposure sources implement preventative measures against development of AA-associated cancers. Herein, we report a simple efficient iron powder-packed reduction column allows online post-column conversion nonfluorescing its corresponding strongly fluorescing aristolactam (AL), facilitating sensitive selective detection in food grain, arable soil, or groundwater samples high-performance liquid chromatography with fluorescence detection. Moreover, AL, group occurring derivatives demonstrated toxicity cultured bacteria, human cells, rats, monitored quantified simultaneously one single run without sacrificing sensitivity. comparison existing analytical methods measurement, newly developed method not only inexpensive less laborious, but it offers improved We believe this novel will find wide application identifying presence food, medicines, environmental samples, thus assisting identification removal sources.

Language: Английский

Citations

4
Mitochondrial Dysfunction in Aristolochic Acid I-Induced Kidney Diseases: What We Know and What We Do Not Know DOI Creative Commons
Alexandra Teodora Lukinich-Gruia, Crenguta Livia Calma,

Flavia A. E. Szekely

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(17), P. 7961 - 7961

Published: Sept. 6, 2024

Aristolochic acids, compounds derived from Aristolochiaceae plant species, are associated with significant renal nephrotoxicity and carcinogenicity. acid I (AAI), the most predominant potent of these compounds, is a primary etiological agent in acute chronic kidney diseases such as Acid Nephropathy (AAN) Balkan Endemic (BEN). Due to kidneys’ critical role xenobiotic excretion, they organs affected by AAI toxicity. Recent vitro vivo studies have highlighted mitochondrial dysfunction crucial factor pathogenesis diseases. This review provides an update on recent advances understanding causes acquired within context AAN BEN. Key findings include identification DNA depletion, loss membrane potential, decreased ATP production contributors damage. Additionally, oxidative stress markers inflammatory mediators been implicated disease progression. Potential therapeutic approaches, use antioxidants like vitamin C catalpol, shown promise mitigating AAI-induced cytotoxicity. Furthermore, future predictive approaches pharmacogenomics could pave way for novel mitochondria-targeted treatments. A comprehensive characterization function, its underlying molecular mechanisms, specific biomarkers offer valuable insights potential options, significantly impacting current management

Language: Английский

Citations

1
Nephrotoxicity of Natural Products: Aristolochic Acid and Fungal Toxins DOI
Paraskevi Sotiropoulou,

Alexander Zielinski,

Daniel R. Dietrich

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

1
In vitro nephrotoxicity and structure-toxicity relationships of eight natural aristolactams DOI

Shiyu Xue,

Weilian Bao, Jiaren Lyu

et al.

Toxicon, Journal Year: 2024, Volume and Issue: 254, P. 108214 - 108214

Published: Dec. 12, 2024

Language: Английский

Citations

1
Long-term oral administration of Kelisha capsule does not cause hepatorenal toxicity in rats DOI
Suyan Liu, Yong Zhao, Chunying Li

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 332, P. 118320 - 118320

Published: May 11, 2024

Language: Английский

Citations

0
Using 2-(2-Chlorophenyl)thiazolidine-4-carboxylic Acid as a Novel Biomarker for 2-Chlorobenzalmalononitrile Exposure DOI
Guanrui Pan,

Hei-Tak Tse,

Ho‐Wai Chan

et al.

Chemical Research in Toxicology, Journal Year: 2024, Volume and Issue: 37(10), P. 1747 - 1754

Published: Oct. 5, 2024

This study addressed the development of a novel biomarker for 2-chlorobenzalmalononitrile (CS) gas exposure. Using liquid chromatographic and mass spectrometric techniques, we found that CS underwent rapid hydrolysis into 2-chlorobenzaldehyde (2-CBA), highly reactive intermediate reacted swiftly with endogenous cysteine (Cys) Cys residues in proteins, producing stable 2-(2-chlorophenyl)thiazolidine-4-carboxylic acid adduct (ClPh-SPro) high yield, which may be used as exposure dosimeter. In particular, it was most rapidly hydrolyzed under physiologically relevant conditions, over 90% being converted 2-CBA short 20 min. The resultant then (k = 0.086 M–1 s–1), forming thiazolidine-4-carboxylic adduct, detected both intracellular fluid cell-isolated proteins CS-exposed lung cells, well purified human serum albumin. It is expected results this will facilitate assessment bystanders who have been exposed to levels unwillingly.

Language: Английский

Citations

0
Disruption of local circadian clocks in aristolochic acid-induced nephropathy in mice DOI

Dihao Xie,

Simin Zhong,

Meixue Luo

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 135, P. 156235 - 156235

Published: Nov. 9, 2024

Language: Английский

Citations

0