Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(22)
Published: March 3, 2023
Lipopolysaccharides
from
Bacteroides
vulgatus
represent
interesting
targets
for
the
treatment
of
inflammatory
bowel
diseases.
However,
efficient
access
to
long,
branched
and
complex
lipopolysaccharides
remains
challenging.
Herein,
we
report
modular
synthesis
a
tridecasaccharide
vulgates
through
an
orthogonal
one-pot
glycosylation
strategy
based
on
glycosyl
ortho-(1-phenylvinyl)benzoates,
which
avoids
issues
thioglycoside-based
synthesis.
Our
approach
also
features:
1)
5,7-O-di-tert-butylsilylene-directed
stereoselective
construction
α-Kdo
linkage;
2)
hydrogen-bond-mediated
aglycone
delivery
formation
β-mannosidic
bonds;
3)
remote
anchimeric
assistance
assembly
α-fucosyl
4)
several
synthetic
steps
strategic
use
protecting
groups
streamline
oligosaccharide
assembly;
5)
convergent
[1+6+6]
target.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(8), P. 4305 - 4305
Published: April 13, 2024
Endotoxin
is
a
general
term
for
toxic
substances
in
Gram-negative
bacteria,
whose
damaging
effects
are
mainly
derived
from
the
lipopolysaccharides
(LPS)
cell
walls
of
and
strong
pyrogen.
Obesity
chronic,
low-grade
inflammatory
condition,
LPS
thought
to
trigger
exacerbate
it.
The
gut
flora
largest
source
body,
it
increasingly
believed
that
altered
intestinal
microorganisms
can
play
an
essential
role
pathology
different
diseases.
Today,
complex
axis
linking
states
adiposity
has
not
been
well
elucidated.
This
review
summarises
evidence
interconnection
between
LPS,
obesity,
flora,
further
expanding
our
understanding
as
mediator
disease
contributing
lessening
obesity
related
metabolic
disorders.
As
providing
targets
associated
with
hoped
interventions
combine
address
individual
differences
treatment.
Angewandte Chemie International Edition,
Journal Year:
2022,
Volume and Issue:
61(29)
Published: May 11, 2022
Acinetobacter
baumannii
is
currently
posing
a
serious
threat
to
global
health.
Lipopolysaccharide
(LPS)
potent
virulence
factor
of
pathogenic
Gram-negative
bacteria.
To
explore
the
antigenic
properties
A.
LPS,
four
Kdo-containing
inner
core
glycans
from
strain
ATCC
17904
were
synthesized.
A
flexible
and
divergent
method
based
on
use
orthogonally
substituted
α-Kdo-(2→5)-Kdo
disaccharides
was
developed.
Selective
removal
different
protecting
groups
in
these
key
precursors
elongation
sugar
chain
via
α-stereocontrolled
coupling
with
5,7-O-di-tert-butylsilylene
or
5-O-benzoyl
protected
Kdo
thioglycosides
2-azido-2-deoxyglucosyl
thioglycoside
allowed
efficient
assembly
target
molecules.
Glycan
microarray
analysis
sera
infected
patients
revealed
that
4,5-branched
trimer
potential
epitope,
which
attractive
for
further
immunological
research
develop
carbohydrate
vaccines
against
baumannii.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(12), P. 5463 - 5469
Published: March 15, 2023
As
the
entering
of
bacterial
endotoxin
into
blood
can
cause
various
life-threatening
pathological
conditions,
screening
and
detection
low-abundance
are
great
importance
to
human
health.
Taking
advantage
signal
amplification
by
target-assisted
electrochemically
mediated
atom
transfer
radical
polymerization
(teATRP),
we
illustrate
herein
a
simple
cost-effective
electrochemical
aptasensor
capable
detecting
with
high
sensitivity
selectivity.
Specifically,
aptamer
receptor
was
employed
for
selective
capture
endotoxin,
which
glycan
chain
then
decorated
ATRP
initiators
via
covalent
coupling
between
diol
sites
phenylboronic
acid
(PBA)
group,
followed
recruitment
ferrocene
reporters
grafting
polymer
chains
through
potentiostatic
eATRP
under
ambient
temperature.
be
hundreds
while
further
recruit
thousands
each
initiator-decorated
site,
teATRP-based
strategy
allows
dual
signal.
This
dually
amplified
has
ability
sensitively
selectively
detect
at
concentration
as
low
1.2
fg/mL,
its
practical
applicability
been
demonstrated
using
serum
samples.
Owing
simplicity,
efficiency,
biocompatibility,
inexpensiveness
strategy,
this
holds
application
potential
in
sensitive
many
other
chain-containing
bio-targets.
Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(22)
Published: March 3, 2023
Lipopolysaccharides
from
Bacteroides
vulgatus
represent
interesting
targets
for
the
treatment
of
inflammatory
bowel
diseases.
However,
efficient
access
to
long,
branched
and
complex
lipopolysaccharides
remains
challenging.
Herein,
we
report
modular
synthesis
a
tridecasaccharide
vulgates
through
an
orthogonal
one-pot
glycosylation
strategy
based
on
glycosyl
ortho-(1-phenylvinyl)benzoates,
which
avoids
issues
thioglycoside-based
synthesis.
Our
approach
also
features:
1)
5,7-O-di-tert-butylsilylene-directed
stereoselective
construction
α-Kdo
linkage;
2)
hydrogen-bond-mediated
aglycone
delivery
formation
β-mannosidic
bonds;
3)
remote
anchimeric
assistance
assembly
α-fucosyl
4)
several
synthetic
steps
strategic
use
protecting
groups
streamline
oligosaccharide
assembly;
5)
convergent
[1+6+6]
target.
