From nature to industry: Harnessing enzymes for biocatalysis

Science, Journal Year: 2023, Volume and Issue: 382(6673)

Published: Nov. 23, 2023

Biocatalysis harnesses enzymes to make valuable products. This green technology is used in countless applications from bench scale industrial production and allows practitioners access complex organic molecules, often with fewer synthetic steps reduced waste. The last decade has seen an explosion the development of experimental computational tools tailor enzymatic properties, equipping enzyme engineers ability create biocatalysts that perform reactions not present nature. By using (chemo)-enzymatic synthesis routes or orchestrating intricate cascades, scientists can synthesize elaborate targets ranging DNA pharmaceuticals starch made vitro CO2-derived methanol. In addition, new chemistries have emerged through combination biocatalysis transition metal catalysis, photocatalysis, electrocatalysis. review highlights recent key developments, identifies current limitations, provides a future prospect for this rapidly developing technology.

Language: Английский

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Salient Achievements in Synthetic Organic Chemistry Enabled by Mechanochemical Activation

Synthesis, Journal Year: 2023, Volume and Issue: 55(16), P. 2439 - 2459

Published: May 3, 2023

Abstract Although known for millennia, it is only recently that mechanochemistry has received serious attention by chemists. Indeed, during the past 15 years an extraordinary number of reports concerning solid-state chemical transformations through grinding and milling techniques have been recorded. This short review discusses circumstances led this renaissance, highlighting present intense interest in so-called green chemistry, enabling capacity to handle insoluble substrates, identification profound influence additives can on mechanochemically activated reactions. The core account focuses salient developments synthetic organic especially amino acid peptide­ mechanosynthesis, successful employment mechanochemical activation combination with asymmetric organocatalysis, promising enzymatic whole cell biocatalysis, remarkable achievement multicomponent selective reactions via complex, multistep reaction pathways, mechanosynthesis representative heterocycles. final section comments some pending tasks area, such as scaling-up processes be practical use industry, requirement easier more efficient control parameters monitoring devices, consequently careful analysis additional procedures a proper understanding phenomena. 1 Introduction 2 Brief History Mechanochemistry 3 Milling Equipment Reaction Parameters 4 Attributes That Propelled Its Present Renaissance 4.1 Enormous Attention Being Presently Paid Sustainable Chemistry 4.2 Reduced Energy Consumption 4.3 Additive-Based 4.4 Handling Insoluble Reactants 4.5 ‘Impossible’ Reactions Are Successful 4.6 Air- Water-Sensitive Reagents Ball 5 Salient Developments Mechanochemical Activation Synthetic Organic 5.1 Amino Acid Peptide Mechanosynthesis 5.2 Asymmetric Synthesis Organocatalysis under Ball-Milling Conditions 5.3 Mechanoenzymology 5.4 Multicomponent Activated 5.5 Heterocycles Modification 6 Future Directions 6.1 Scaling-Up Protocols 6.2 Temperature-Controlled 6.3 Understanding Transformations 6.4 Emerging Techniques 7 Conclusions

Language: Английский

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Smart chemistry and applied perceptions of enzyme-coupled nano-engineered assemblies to meet future biocatalytic challenges

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 493, P. 215329 - 215329

Published: July 9, 2023

Language: Английский

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One-pot chemo- and photo-enzymatic linear cascade processes

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(15), P. 7875 - 7938

Published: Jan. 1, 2024

This review explores linear cascade reactions by combining chemo-, photo- and biocatalysts for organic synthesis strategies to overcome incompatibility issues.

Language: Английский

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Molecular Engineering and Morphology Control of Covalent Organic Frameworks for Enhancing Activity of Metal‐Enzyme Cascade Catalysis

Advanced Science, Journal Year: 2024, Volume and Issue: 11(25)

Published: April 23, 2024

Abstract Metal‐enzyme integrated catalysts (MEICs) that combine metal and enzyme offer great potential for sustainable chemoenzymatic cascade catalysis. However, rational design construction of optimal microenvironments accessible active sites in individual nanostructures are necessary but still challenging. Herein, Pd nanoparticles (NPs) Candida antarctica lipase B (CALB) co‐immobilized into the pores surfaces covalent organic frameworks (COFs) with tunable functional groups, affording Pd/COF‐X/CALB (X = ONa, OH, OMe) MEICs. This strategy can regulate microenvironment around NPs CALB, their interactions substrates. As a result, activity COF‐based MEICs catalyzing dynamic kinetic resolution primary amines is enhanced followed COF‐OMe > COF‐OH COF‐ONa. The experimental simulation results demonstrated groups COFs modulated conformation electronic states NPs, affinity to substrate, which contributed improvement catalytic Further, prepared using COF hollow structure as support material, increased mass transfer efficiency, thus improving performance. work provides blueprint preparation highly

Language: Английский

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Status check: biocatalysis; its use with and without chemocatalysis. How does the fine chemicals industry view this area?

Green Chemistry, Journal Year: 2023, Volume and Issue: 25(16), P. 6092 - 6107

Published: Jan. 1, 2023

Stages of the penetration catalytic technology and necessity for fruitful collaboration.

Language: Английский

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Stereodivergent Protein Engineering of Fatty Acid Photodecarboxylase for Light‐Driven Kinetic Resolution of Sec‐Alcohol Oxalates

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(10)

Published: Jan. 10, 2024

Stereodivergent engineering of one enzyme to create stereocomplementary variants for synthesizing optically pure molecules with tailor-made (R) or (S) configurations on an optional basis is highly desirable and challenging. This study aimed engineer fatty acid photodecarboxylase from Chlorella variabilis (CvFAP) using the focused rational iterative site-specific mutagenesis (FRISM) strategy obtain two excellent selectivity (both giving products up 99 % e.e.). These were used CvFAP-catalyzed light-driven kinetic resolution oxalates oxamic acids prepared corresponding sec-alcohols amines, providing a new biotransformation process preparing chiral amines. Molecular dynamics simulation, data transient spectra revealed source selectivity. represents first example amines catalyzed by pair CvFAPs.

Language: Английский

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A review of nanotechnology in enzyme cascade to address challenges in pre-treating biomass

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 270, P. 132466 - 132466

Published: May 16, 2024

Language: Английский

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Carbonyl Olefin Metathesis and Dehydrogenative Cyclization of Aromatic Ketones and gem‐Difluoroalkenes

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 63(5)

Published: Dec. 9, 2023

The beauty of one-pot cascade reaction lies in the efficient disconnection and construction several bonds a single flask, without isolation any intermediates. Herein, we report first photoinduced thermally promoted reactions readily available aromatic ketones gem-difluoroalkenes for synthesis phenanthrenes which possess potential utility drug design materials science. combines carbonyl-olefin metathesis (cascade [2+2] cyclization controlled retro cyclization) dehydrogenative conrotatory 6π electrocyclization collidine-promoted aromatization) together one pot. oxidant-free, acid-free metal-free shows broad substrate scope wide functional group tolerance.

Language: Английский

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Co-immobilization of enzymes and chemocatalysts for one-pot chemoenzymatic cascades: Scaffold engineering toward more efficient catalysis

Chem Catalysis, Journal Year: 2024, Volume and Issue: 4(3), P. 100894 - 100894

Published: Jan. 21, 2024

Language: Английский

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