Disordered proteins interact with the chemical environment to tune their protective function during drying DOI Creative Commons

Shraddha KC,

Kenny Nguyen,

Vincent Nicholson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 2, 2024

Abstract The conformational ensemble and function of intrinsically disordered proteins (IDPs) are sensitive to their solution environment. inherent malleability combined with the exposure residues accounts for this sensitivity. One context in which IDPs play important roles that is concomitant massive changes intracellular environment during desiccation (extreme drying). ability organisms survive has long been linked accumulation high levels cosolutes such as trehalose or sucrose well enrichment IDPs, late embryogenesis abundant (LEA) cytoplasmic heat soluble (CAHS) proteins. Despite knowing co-enriched alongside endogenous, species-specific desiccation, little known mechanistically about how IDP-cosolute interactions influence tolerance. Here, we test notion protective desiccation-related enhanced through induced by endogenous cosolutes. We find derived from four different spanning two LEA protein families CAHS family, synergize best drying promote protection. Yet structural parameters do not correlate synergy either further demonstrate CAHS, but proteins, related self-assembly formation a gel. Our results suggest functional between convergent protection strategy seen among IDP organisms, yet, mechanisms underlying differ families.

Language: Английский

Molecular determinants of condensate composition DOI Creative Commons
Alex S. Holehouse, Simon Alberti

Molecular Cell, Journal Year: 2025, Volume and Issue: 85(2), P. 290 - 308

Published: Jan. 1, 2025

Language: Английский

Citations

4
Biomolecular condensates regulate cellular electrochemical equilibria DOI
Yifan Dai, Zhengqing Zhou,

Yu Wen

et al.

Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

15
Three-dimensional genome architecture persists in a 52,000-year-old woolly mammoth skin sample DOI Creative Commons
Marcela Sandoval‐Velasco, Olga Dudchenko, Juan Antonio Rodríguez

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(14), P. 3541 - 3562.e51

Published: July 1, 2024

Analyses of ancient DNA typically involve sequencing the surviving short oligonucleotides and aligning to genome assemblies from related, modern species. Here, we report that skin a female woolly mammoth (†Mammuthus primigenius) died 52,000 years ago retained its architecture. We use PaleoHi-C map chromatin contacts assemble genome, yielding 28 chromosome-length scaffolds. Chromosome territories, compartments, loops, Barr bodies, inactive X chromosome (Xi) superdomains persist. The active compartments in more closely resemble Asian elephant than other tissues. Our analyses uncover new biology. Differences compartmentalization reveal genes whose transcription was potentially altered mammoths vs. elephants. Mammoth Xi has tetradic architecture, not bipartite like human mouse. hypothesize that, shortly after this mammoth's death, sample spontaneously freeze-dried Siberian cold, leading glass transition preserved subfossils chromosomes at nanometer scale.

Language: Английский

Citations

13
Unlocking the electrochemical functions of biomolecular condensates DOI
Yifan Dai, Zhen‐Gang Wang, Richard N. Zare

et al.

Nature Chemical Biology, Journal Year: 2024, Volume and Issue: 20(11), P. 1420 - 1433

Published: Sept. 26, 2024

Language: Английский

Citations

13
A cytoplasmic osmosensing mechanism mediated by molecular crowding–sensitive DCP5 DOI
Zhenyu Wang, Qiuhua Yang, Dan Zhang

et al.

Science, Journal Year: 2024, Volume and Issue: 386(6721)

Published: Oct. 31, 2024

Plants are frequently challenged by osmotic stresses. How plant cells sense environmental osmolarity changes is not fully understood. We report that Arabidopsis Decapping 5 (DCP5) functions as a multifunctional cytoplasmic osmosensor senses and responds to extracellular hyperosmolarity. DCP5 harbors plant-specific intramolecular crowding sensor (ICS) undergoes conformational change drives phase separation in response osmotically intensified molecular crowding. Upon hyperosmolarity exposure, rapidly reversibly assembles DCP5-enriched stress granules (DOSGs), which sequestrate plenty of mRNA regulatory proteins, thus adaptively reprograms both the translatome transcriptome facilitate adaptation. Our findings uncover osmosensing mechanism mediated with sensitivity suggest sensory function for hyperosmotically induced granules.

Language: Английский

Citations

12
Aging-dependent evolving electrochemical potentials of biomolecular condensates regulate their physicochemical activities DOI
Wen Yu,

Xiao Guo,

Yu Xia

et al.

Nature Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Language: Английский

Citations

1
Phase separation of multicomponent peptide mixtures into dehydrated clusters with hydrophilic cores DOI

William Hill Brown,

Davit A. Potoyan

Biophysical Journal, Journal Year: 2024, Volume and Issue: 123(3), P. 349 - 360

Published: Jan. 1, 2024

Language: Английский

Citations

8
Protecting Proteins from Desiccation Stress Using Molecular Glasses and Gels DOI Creative Commons
Gil I. Olgenblum,

Brent O. Hutcheson,

Gary J. Pielak

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(9), P. 5668 - 5694

Published: April 18, 2024

Faced with desiccation stress, many organisms deploy strategies to maintain the integrity of their cellular components. Amorphous glassy media composed small molecular solutes or protein gels present general for protecting against drying. We review these and proposed mechanisms explain protection in a vitreous matrix under conditions low hydration. also describe efforts exploit similar technological applications proteins dry highly desiccated states. Finally, we outline open questions possibilities future explorations.

Language: Английский

Citations

8
The major inducible small heat shock protein HSP20-3 in the tardigrade Ramazzottius varieornatus forms filament-like structures and is an active chaperone DOI Creative Commons

Mohammad Al-Ansari,

Taylor Fitzsimons,

Wenbin Wei

et al.

Cell Stress and Chaperones, Journal Year: 2024, Volume and Issue: 29(1), P. 51 - 65

Published: Feb. 1, 2024

The tardigrade Ramazzottius varieornatus has remarkable resilience to a range of environmental stresses. In this study, we have characterised two members the small heat shock protein (sHSP) family in R. varieornatus, HSP20-3 and HSP20-6. These are most highly upregulated sHSPs response 24 h at 35

Language: Английский

Citations

6
Molecular Crowding: Physiologic Sensing and Control DOI
Arohan R. Subramanya, Cary R. Boyd‐Shiwarski

Annual Review of Physiology, Journal Year: 2023, Volume and Issue: 86(1), P. 429 - 452

Published: Nov. 6, 2023

The cytoplasm is densely packed with molecules that contribute to its nonideal behavior. Cytosolic crowding influences chemical reaction rates, intracellular water mobility, and macromolecular complex formation. Overcrowding potentially catastrophic; counteract this problem, cells have evolved acute chronic homeostatic mechanisms optimize cellular crowdedness. Here, we provide a physiology-focused overview of molecular crowding, highlighting contemporary advances in our understanding sensing control. Long hypothesized as form crowding-induced microcompartmentation, phase separation allows detect respond through the action biomolecular condensates, indicated by recent studies. Growing evidence indicates closely tied cell size fluid volume, responses physical compression desiccation, tissue architecture, circadian rhythm, aging, transepithelial transport, total body electrolyte balance. Thus, fundamental physiologic parameter impacts diverse functions extending from molecule organism.

Language: Английский

Citations

16