Reaching New Heights in Genetic Code Manipulation with High Throughput Screening DOI
Briana R. Lino, Sean Williams,

Michelle E. Castor

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(21), P. 12145 - 12175

Published: Oct. 17, 2024

The chemical and physical properties of proteins are limited by the 20 canonical amino acids. Genetic code manipulation allows for incorporation noncanonical acids (ncAAs) that enhance or alter protein functionality. This review explores advances in three main strategies introducing ncAAs into biosynthesized proteins, focusing on role high throughput screening these advancements. first section discusses engineering aminoacyl-tRNA synthetases (aaRSs) tRNAs, emphasizing how novel selection methods improve characteristics including ncAA efficiency selectivity. second examines high-throughput techniques improving translation machinery, enabling accommodation alternative genetic codes. includes opportunities to through cellular components unrelated translation. final highlights various discovery platforms ncAA-containing showcasing innovative binding ligands enzymes challenging create with only These have led promising drug leads biocatalysts. Overall, ability discover unexpected functionalities significantly influences its applications. Future innovations experimental techniques, along advancements computational design machine learning, poised further elevate this field.

Language: Английский

Autonomous Nucleic Acid and Protein Nanocomputing Agents Engineered to Operate in Living Cells DOI Creative Commons
Martin Panigaj, Tanaya Basu Roy, Elizabeth Skelly

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

In recent years, the rapid development and employment of autonomous technology have been observed in many areas human activity. Autonomous can readily adjust its function to environmental conditions enable an efficient operation without control. While applying same concept designing advanced biomolecular therapies would revolutionize nanomedicine, design approaches engineering biological nanocomputing agents for predefined operations within living cells remain a challenge. made nucleic acids proteins are appealing idea, two decades research has shown that engineered act under real physical biochemical constraints logical manner. Throughout all domains life, perform variety vital functions, where sequence-defined structures these biopolymers either operate on their own or efficiently together. This programmability synergy inspire massive efforts utilize versatility amino encode functions properties otherwise do not exist nature. Perspective covers key concepts used application discusses potential limitations paths forward.

Language: Английский

Citations

2
Emerging Approaches to Investigating Functional Protein Dynamics in Modular Redox Enzymes: Nitric Oxide Synthase as a Model System DOI Creative Commons
Ting Jiang, Megan C. Thielges, Changjian Feng

et al.

Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108282 - 108282

Published: Feb. 1, 2025

Language: Английский

Citations

0
19F NMR study of proteins with parallel incorporation of multiple fluorinated aromatic amino acids DOI Creative Commons
Manman Li,

Guohua Xu,

Qiong Wu

et al.

Magnetic Resonance Letters, Journal Year: 2025, Volume and Issue: unknown, P. 200193 - 200193

Published: Feb. 1, 2025

Language: Английский

Citations

0
Interdisciplinary Approaches to Leverage Biomarker Discovery for Cancer Treatment DOI
Fatemeh Khatami, Nima Rezaei

Interdisciplinary cancer research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Simultaneous measurement of multiple fluorine labelling effect on GB1 stability by 19F NMR DOI
Manman Li,

Guohua Xu,

Zhou Gong

et al.

Talanta, Journal Year: 2025, Volume and Issue: unknown, P. 127959 - 127959

Published: March 1, 2025

Language: Английский

Citations

0
Impact of Fluorine in Manganese Citrate Synthesis on Structure and Value-Added Decomposition Products DOI Creative Commons
Aljaž Škrjanc,

Emanuela Trstenjak,

Mojca Opresnik

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(8), P. 1794 - 1794

Published: April 16, 2025

Synthesis of manganese citrates was carried out in the presence and absence fluoride ions. The HF or NaF led to formation new layered whose crystal structures were determined by single-crystal X-ray diffraction. prepared single-phase with without ions then used as precursors for preparation mesoporous MnxOy hierarchically porous MnxOy@C phases, which could be interest catalytic, sorption, electrochemical applications.

Language: Английский

Citations

0
Reaching New Heights in Genetic Code Manipulation with High Throughput Screening DOI
Briana R. Lino, Sean Williams,

Michelle E. Castor

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(21), P. 12145 - 12175

Published: Oct. 17, 2024

The chemical and physical properties of proteins are limited by the 20 canonical amino acids. Genetic code manipulation allows for incorporation noncanonical acids (ncAAs) that enhance or alter protein functionality. This review explores advances in three main strategies introducing ncAAs into biosynthesized proteins, focusing on role high throughput screening these advancements. first section discusses engineering aminoacyl-tRNA synthetases (aaRSs) tRNAs, emphasizing how novel selection methods improve characteristics including ncAA efficiency selectivity. second examines high-throughput techniques improving translation machinery, enabling accommodation alternative genetic codes. includes opportunities to through cellular components unrelated translation. final highlights various discovery platforms ncAA-containing showcasing innovative binding ligands enzymes challenging create with only These have led promising drug leads biocatalysts. Overall, ability discover unexpected functionalities significantly influences its applications. Future innovations experimental techniques, along advancements computational design machine learning, poised further elevate this field.

Language: Английский

Citations

1