Cutting-edge bioorthogonal chemistry: Innovations, practical applications, and emerging trends DOI Open Access
Anup Basnet Chetry

European Journal of Chemistry, Journal Year: 2024, Volume and Issue: 15(4), P. 355 - 365

Published: Dec. 31, 2024

Bioorthogonal chemistry has emerged as a pivotal field in molecular science, offering transformative tools for applications drug discovery, imaging, and biology. This review provides comprehensive analysis of recent advancements bioorthogonal chemistry, emphasizing key innovations, practical applications, future research directions. We explore state-of-the-art reactions, including Staudinger ligation, strain-promoted azide-alkyne cycloaddition (SPAAC), tetrazine detailing their mechanisms, advantages, limitations. The highlights significant innovations such novel fluorogenic probes, improved catalysts, enhanced reaction conditions that have expanded the utility efficiency these reactions. Practical are examined, showing how advances revolutionized fields like live-cell targeted delivery, labeling. Looking to future, we discuss emerging trends potential avenues, integration with other advanced technologies development new methodologies. detailed overview current state outlines its potential, serving valuable resource researchers practitioners field.

Language: Английский

Strategies to Expand the Genetic Code of Mammalian Cells DOI
Arianna O. Osgood, Zeyi Huang, Kaitlyn H. Szalay

et al.

Chemical Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Genetic code expansion (GCE) in mammalian cells has emerged as a powerful technology for investigating and engineering protein function. This method allows the precise incorporation of rapidly growing toolbox noncanonical amino acids (ncAAs) into predefined sites target proteins expressed living cells. Due to minimal size these genetically encoded ncAAs, wide range functionalities they provide, ability introduce them freely at virtually any site by simple mutagenesis, this holds immense potential probing complex biology next-generation biotherapeutics. In review, we provide an overview underlying machinery that enables ncAA mutagenesis how are developed. We have also compiled updated list ncAAs been successfully incorporated Finally, our perspectives on current challenges need be addressed fully harness technology.

Language: Английский

Citations

1
Expanding the genetic code: In vivo approaches for incorporating non-proteinogenic monomers DOI
Dongheon Lee,

S Yun,

Jong‐il Choi

et al.

The Journal of Microbiology, Journal Year: 2025, Volume and Issue: 63(3), P. e2501005 - e2501005

Published: March 28, 2025

Language: Английский

Citations

1
Noncanonical Amino Acid Incorporation in Animals and Animal Cells DOI
Joo‐Chan Kim,

YouJin Kim,

Suho Cho

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(22), P. 12463 - 12497

Published: Nov. 14, 2024

Noncanonical amino acids (ncAAs) are synthetic building blocks that, when incorporated into proteins, confer novel functions and enable precise control over biological processes. These small yet powerful tools offer unprecedented opportunities to investigate manipulate various complex life forms. In particular, ncAA incorporation technology has garnered significant attention in the study of animals their constituent cells, which serve as invaluable model organisms for gaining insights human physiology, genetics, diseases. This review will provide a comprehensive discussion on applications animal covering past achievements, current developments, future perspectives.

Language: Английский

Citations

0
Investigation of protein post-translational modifications with site-specifically incorporated non-canonical amino acids DOI
Jiayu Gu,

Lihui Lao,

Yulin Chen

et al.

Bioorganic & Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 117, P. 118013 - 118013

Published: Nov. 21, 2024

Language: Английский

Citations

0
Pyridoxal 5′-Phosphate (PLP)-Dependent β- and γ-Substitution Reactions Forming Nonproteinogenic Amino Acids in Natural Product Biosynthesis DOI
Taku Mizutani, Ikuro Abe

Journal of Natural Products, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

Living organisms synthesize various nonproteinogenic amino acids (NPAAs) as the building blocks of natural products. These NPAAs are often biosynthesized by pyridoxal 5′-phosphate (PLP)-dependent enzymes, which catalyze β- or γ- substitutions. enzymes contribute to structural diversification installing new functional groups acid side chains. Recent developments in genome mining have led identification PLP-dependent catalyzing substitutions, form secondary metabolism. This short review summarizes recently investigated PLP-enzymes γ-substitutions biosynthesis covering literature published from 2015 through 2024.

Language: Английский

Citations

0
Cutting-edge bioorthogonal chemistry: Innovations, practical applications, and emerging trends DOI Open Access
Anup Basnet Chetry

European Journal of Chemistry, Journal Year: 2024, Volume and Issue: 15(4), P. 355 - 365

Published: Dec. 31, 2024

Bioorthogonal chemistry has emerged as a pivotal field in molecular science, offering transformative tools for applications drug discovery, imaging, and biology. This review provides comprehensive analysis of recent advancements bioorthogonal chemistry, emphasizing key innovations, practical applications, future research directions. We explore state-of-the-art reactions, including Staudinger ligation, strain-promoted azide-alkyne cycloaddition (SPAAC), tetrazine detailing their mechanisms, advantages, limitations. The highlights significant innovations such novel fluorogenic probes, improved catalysts, enhanced reaction conditions that have expanded the utility efficiency these reactions. Practical are examined, showing how advances revolutionized fields like live-cell targeted delivery, labeling. Looking to future, we discuss emerging trends potential avenues, integration with other advanced technologies development new methodologies. detailed overview current state outlines its potential, serving valuable resource researchers practitioners field.

Language: Английский

Citations

0