Integration of Fluorescence Spectroscopy into a Photobioreactor for the Monitoring of Cyanobacteria

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 128 - 128

Published: Feb. 20, 2025

Phytoplankton are essential to aquatic ecosystems but can cause harmful algal blooms (HABs) that threaten water quality, life, and human health. Developing new devices based on spectroscopic techniques offers a promising alternative for rapid accurate monitoring of environments. However, phytoplankton undergo various physiological changes throughout their life cycle, leading alterations in optical properties, such as autofluorescence. In this study, we present modification low-cost photobioreactor designed implement fluorescence spectroscopy analyze the evolution spectral signals during growth cycles. This device primarily facilitates characterization autofluorescence, providing valuable information development future detecting phytoplankton. Additionally, real-time testing was performed cyanobacterial cultures, where autofluorescence were observed under different conditions. work demonstrates cost-effective implementation within photobioreactor, offering preliminary analysis functional field ecosystems.

Language: Английский

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Untargeted Metabolomic Analysis and Cytotoxicity of Extracts of the Marine Dinoflagellate Amphidinium eilatiense Against Human Cancer Cell Lines

Toxins, Journal Year: 2025, Volume and Issue: 17(4), P. 150 - 150

Published: March 21, 2025

Members of the benthic marine dinoflagellate genus Amphidinium produce a variety bioactive compounds, exhibiting potent cytotoxicity in cell assays. Crude methanolic extracts from three genetically distinct cultured strains A. eilatiense J.J. Lee were screened for against human breast and four lung cancer lines to evaluate potential applications anticancer therapy. A standard tetrazolium viability assay demonstrated that crude extract (100 µg mL−1) strain AeSQ181 reduced by 20–35% five lines. Further bioassay-guided fractionation these yielded non-polar fractions (FNP-5 FNP-6) with particularly high cytotoxic activity (H1563) (MDA-MB-231) adenocarcinoma Untargeted metabolomic analysis liquid chromatography coupled high-resolution mass spectrometry (LC-HRMS) revealed much richer chemical diversity profile than previous toxigenicity studies on exclusively focused linear cyclic polyethers their macrolide analogs as putative cytotoxins. This untargeted study showed substantial differences composition between biologically active non-active fractions. Preliminary biological characterization confirms this species is rich source natural products such therapeutics.

Language: Английский

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Ab Initio Simulation of Carbon Dioxide Capture by Phosphonium-Based Carbanion Ionic Liquids: Effects of a Cation and an Anion

Published: Jan. 1, 2025

Language: Английский

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