Immobilized metal ion affinity chromatography: waltz of metal ions and biomacromolecules DOI
Rui Yan, Yan‐Ming Xu, Andy T. Y. Lau

et al.

Expert Review of Proteomics, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Immobilized metal ion affinity chromatography (IMAC) is an effective method developed in the 1980s for separation and purification of proteins. The system consists a solid-phase matrix, linking ligand, ion. based on ability ions to bind specifically certain specific amino acid residues proteins, thereby selectively enriching purifying This review aims describe current knowledge fundamental principle IMAC summarize supports, chelating ligands, IMAC. In addition, how technology used proteomics DNA research are highlighted. Over past decades, has been extensively utilized as predominant technique protein enrichment variety biological medical research, such disease diagnosis, tumor biomarker identification, purification, nucleic research. future, should be integrated with other technologies promote applications metalloproteomes metallodrug development clinical translation.

Language: Английский

Dithiophene chemosensor for ultrasensitive intracellular detection of Al3+: Design, DFT analysis, and ESIPT-PET mechanisms DOI
Peng Luo, Lu Liu, Hong Chen

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: 339, P. 126244 - 126244

Published: April 15, 2025

Language: Английский

Citations

0
Immobilized metal ion affinity chromatography: waltz of metal ions and biomacromolecules DOI
Rui Yan, Yan‐Ming Xu, Andy T. Y. Lau

et al.

Expert Review of Proteomics, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Immobilized metal ion affinity chromatography (IMAC) is an effective method developed in the 1980s for separation and purification of proteins. The system consists a solid-phase matrix, linking ligand, ion. based on ability ions to bind specifically certain specific amino acid residues proteins, thereby selectively enriching purifying This review aims describe current knowledge fundamental principle IMAC summarize supports, chelating ligands, IMAC. In addition, how technology used proteomics DNA research are highlighted. Over past decades, has been extensively utilized as predominant technique protein enrichment variety biological medical research, such disease diagnosis, tumor biomarker identification, purification, nucleic research. future, should be integrated with other technologies promote applications metalloproteomes metallodrug development clinical translation.

Language: Английский

Citations

0