Chemical biology approaches to resolve the subcellular GPCR signaling landscape DOI
Andreas Böck, Braden T. Lobingier, Miriam Stoeber

et al.

Nature Chemical Biology, Journal Year: 2025, Volume and Issue: unknown

Published: June 2, 2025

Language: Английский

State-dependent motion of a genetically encoded fluorescent biosensor DOI Creative Commons
Paul C. Rosen, Samantha M. Horwitz, Daniel J. Brooks

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(10)

Published: March 6, 2025

Genetically encoded biosensors can measure biochemical properties such as small-molecule concentrations with single-cell resolution, even in vivo. Despite their utility, these sensors are "black boxes": Very little is known about the structures of low- and high-fluorescence states or what features required to transition between them. We used LiLac, a lactate biosensor quantitative fluorescence-lifetime readout, model system address questions. X-ray crystal engineered high-affinity metal bridges demonstrate that LiLac exhibits large interdomain twist motion pulls fluorescent protein away from "sealed," high-lifetime state absence "cracked," low-lifetime its presence. Understanding dynamics will help think engineer other biosensors.

Language: Английский

Citations

1
Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks DOI
Michelle S. Frei, S Sanchez, Xinchang He

et al.

Nature Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Language: Английский

Citations

1
Advancing super-resolution microscopy with metal complexes: functional imaging agents for nanoscale visualization DOI

Nur Aininie Yusoh,

Martin R. Gill, Xiaohe Tian

et al.

Chemical Society Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review highlights recent advancements in the use of metal complexes as functional imaging agents for nanoscale via super-resolution microscopy.

Language: Английский

Citations

0
Advancing Biosensing through Super-Resolution Fluorescence Microscopy DOI

Ga‐eun Go,

Doory Kim

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117374 - 117374

Published: March 1, 2025

Language: Английский

Citations

0
Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies DOI Creative Commons
Lei Zhang,

Dongwen Shen,

Jia-Zhen Yang

et al.

Chemical & Biomedical Imaging, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Illuminating plant metabolism with genetically encoded biosensors DOI Creative Commons
Stephan Wagner, Andreas J. Meyer

Journal of Plant Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 154498 - 154498

Published: April 1, 2025

Language: Английский

Citations

0
High-performance genetically-encoded green and red fluorescent biosensors for pyruvate DOI Creative Commons
Shosei Imai, Saaya Hario,

Carl Suerte

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Abstract Pyruvate is the end-product of glycolysis and a central metabolite involved in many biochemical pathways. However, lack high-performance (i.e., Δ F / 0 > 10) single fluorescent protein (FP)-based biosensors has hindered efforts to investigate physiological role pyruvate. Here, we present GreenPy1 ApplePy1 series, which are green FP (GFP)-based red (RFP)-based pyruvate biosensors, respectively. Both series exhibit large fluorescence intensity change (Δ ∼ 20 >40) range affinities (10s μM several mM). We demonstrate utility these for multicolor imaging concentration changes mammalian cells.

Language: Английский

Citations

0
Mechanism and application of thiol–disulfide redox biosensors with a fluorescence-lifetime readout DOI Creative Commons
Paul C. Rosen,

Andrew Glaser,

Juan Ramón Martínez‐François

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(19)

Published: May 6, 2025

Genetically encoded biosensors with changes in fluorescence lifetime (as opposed to intensity) can quantify small molecules complex contexts, even vivo. However, lifetime-readout sensors are poorly understood at a molecular level, complicating their development. Although there many that have fluorescence-intensity changes, currently only few fluorescence-lifetime changes. Here, we optimized two for thiol–disulfide redox (RoTq-Off and RoTq-On) opposite response oxidation. Using biophysical approaches, showed the high-lifetime states of these lock chromophore more firmly place than low-lifetime do. Two-photon imaging RoTq-On fused glutaredoxin (Grx1) enabled robust, straightforward monitoring cytosolic glutathione state acute mouse brain slices. The motional mechanism described here is probably common may inform design other sensors; Grx1-RoTq-On fusion sensor will be useful studying physiology.

Language: Английский

Citations

0
Advances and Challenges in Fluorescence‐Based Biosensors for Biological and Chemical Detection DOI
Salha Alharthi, Sarah Alharthi, Mohamed Madani

et al.

ChemBioEng Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 19, 2025

Abstract Fluorescence‐based biosensors have become highly effective analytical instruments, revolutionizing the landscape of biological and chemical detection across diverse fields. These devices harness unique properties fluorescence to detect quantify a wide array analytes with remarkable sensitivity specificity. By integrating recognition elements advanced optical technologies, fluorescence‐based provide high sensitivity, rapid detection, real‐time monitoring capabilities for diagnostic applications. This review discusses fundamental principles biosensing, its key advantages, various types. The applications these sensors span fields such as medicine, environment, food safety, biosecurity, agriculture. Although offer numerous benefits, also addresses ongoing challenges limitations researchers are working overcome, photobleaching, background autofluorescence, limited tissue penetration depth. emphasizes critical design considerations continuous advancements in developing innovative tools, which poised transform future detection.

Language: Английский

Citations

0
Chemical biology approaches to resolve the subcellular GPCR signaling landscape DOI
Andreas Böck, Braden T. Lobingier, Miriam Stoeber

et al.

Nature Chemical Biology, Journal Year: 2025, Volume and Issue: unknown

Published: June 2, 2025

Language: Английский

Citations

0