The Journal of Physical Chemistry B,
Journal Year:
2019,
Volume and Issue:
123(29), P. 6133 - 6149
Published: May 1, 2019
Remarkable
success
in
engineering
novel
efficient
biomarkers
based
on
fluorescent
and
photoactive
proteins
provokes
a
question
of
whether
computational
modeling
their
properties
can
contribute
to
this
important
field.
In
Feature
Article,
we
analyze
selected
papers
devoted
computer
simulations
three
types
systems:
the
green
protein
its
derivatives,
flavin-binding
proteins,
phytochrome
domains.
The
main
emphasis
is
structures,
optical
spectra,
chemical
reactions
chromophore-containing
pockets.
Quantum
chemistry,
quantum
mechanics/molecular
mechanics,
molecular
dynamics
methods
are
effective
tools
these
simulations.
We
highlight
both
stories
persisting
challenges,
discussing
ways
elevating
theoretical
approaches
level
testable
predictions.
Chemical Reviews,
Journal Year:
2019,
Volume and Issue:
119(3), P. 2043 - 2086
Published: Feb. 5, 2019
Singlet
oxygen
(1O2)
is
a
biologically
relevant
reactive
species
capable
of
efficiently
reacting
with
cellular
constituents.
The
resulting
oxidatively
generated
damage
to
nucleic
acids,
membrane
unsaturated
lipids,
and
protein
components
has
been
shown
be
implicated
in
several
diseases,
including
arthritis,
cataracts,
skin
cancer.
may
endogenously
produced,
among
various
possibilities,
by
myeloperoxidase,
an
enzyme
inflammation
processes,
also
the
UVA
component
solar
radiation
through
photosensitization
reactions.
Emphasis
placed
this
Review
on
description
main
oxidation
reactions
initiated
1O2
modifications
within
key
targets,
guanine
for
targeted
amino
acids.
Most
these
give
rise
peroxides
dioxetanes,
whose
formation
rationalized
terms
[4+2]
cycloaddition
1,2-cycloaddition
dienes
+
olefins,
respectively.
use
[18O]-labeled
thermolabile
endoperoxides
as
source
applied
study
mechanistic
aspects
preferential
targets
biological
systems.
A
major
topic
deals
search
molecular
signature
biomolecules
cells.
It
anticipated
that
labeled
association
sensitive
mass
spectrometric
methods
should
constitute
powerful
tools
purpose.
ACS Catalysis,
Journal Year:
2019,
Volume and Issue:
9(5), P. 4115 - 4144
Published: April 9, 2019
Light
has
received
increased
attention
for
various
chemical
reactions
but
also
in
combination
with
biocatalytic
reactions.
Because
currently
only
a
few
enzymatic
are
known,
which
per
se
require
light,
most
transformations
involving
light
and
biocatalyst
exploit
either
providing
the
cosubstrate
or
cofactor
an
appropriate
redox
state
biotransformation.
In
selected
cases,
promiscuous
activity
of
known
enzymes
presence
could
be
induced.
other
approaches,
light-induced
have
been
combined
step,
were
linear
cascade.
Finally,
switchable
moiety
investigated
to
turn
off/on
tune
actual
reaction.
This
Review
gives
overview
approaches
using
biocatalysis.
Physiological Reviews,
Journal Year:
2022,
Volume and Issue:
102(3), P. 1263 - 1325
Published: Jan. 24, 2022
Optogenetics
combines
light
and
genetics
to
enable
precise
control
of
living
cells,
tissues,
organisms
with
tailored
functions.
has
the
advantages
noninvasiveness,
rapid
responsiveness,
tunable
reversibility,
superior
spatiotemporal
resolution.
Following
initial
discovery
microbial
opsins
as
light-actuated
ion
channels,
a
plethora
naturally
occurring
or
engineered
photoreceptors
photosensitive
domains
that
respond
at
varying
wavelengths
ushered
in
next
chapter
optogenetics.
Through
protein
engineering
synthetic
biology
approaches,
genetically
encoded
photoswitches
can
be
modularly
into
scaffolds
host
cells
myriad
biological
processes,
well
behavioral
disease
intervention
vivo.
Here,
we
summarize
these
optogenetic
tools
on
basis
their
fundamental
photochemical
properties
better
inform
chemical
design
principles.
We
also
highlight
exemplary
applications
opsin-free
optogenetics
dissecting
cellular
physiology
(designated
"optophysiology")
describe
current
progress,
future
trends,
wireless
optogenetics,
which
enables
remote
interrogation
physiological
processes
minimal
invasiveness.
This
review
is
anticipated
spark
novel
thoughts
next-generation
devices
promise
accelerate
both
basic
translational
studies.
Advanced Science,
Journal Year:
2020,
Volume and Issue:
7(8)
Published: Feb. 27, 2020
Abstract
Light‐based
technique,
including
optical
imaging
and
photoregulation,
has
become
one
of
the
most
important
tools
for
both
fundamental
research
clinical
practice,
such
as
cell
signal
sensing,
cancer
diagnosis,
tissue
engineering,
drug
delivery,
visual
regulation,
neuromodulation,
disease
treatment.
In
particular,
low
energy
near‐infrared
(NIR,
700–1700
nm)
light
possesses
lower
phototoxicity
higher
penetration
depth
in
living
systems
compared
with
ultraviolet/visible
light,
making
it
a
promising
tool
vivo
applications.
Currently,
NIR
light‐based
photoregulation
strategies
have
offered
possibility
to
real‐time
sense
and/or
modulate
specific
cellular
events
deep
tissues
subcellular
accuracy.
Herein,
recent
progress
respect
monitoring
modulating
spatiotemporal
dynamics
functions
are
summarized.
applications
techniques
theranostics,
regenerative
medicine,
neuroscience
systematically
introduced
discussed.
addition,
challenges
prospects
sensing
regulating
comprehensively
