Phosphate limitation enhances malic acid production on nitrogen-rich molasses with Ustilago trichophora

Biotechnology for Biofuels and Bioproducts, Journal Year: 2024, Volume and Issue: 17(1)

Published: July 3, 2024

Abstract Background An important step in replacing petrochemical products with sustainable, cost-effective alternatives is the use of feedstocks other than, e.g., pure glucose fermentative production platform chemicals. Ustilaginaceae offer advantages a wide substrate spectrum and naturally produce versatile range value-added compounds under nitrogen limitation. A promising candidate dicarboxylic acid malic acid, which may be applied as an acidulant food industry, chelating agent pharmaceuticals, or biobased polymer production. However, fermentable residue streams from agricultural industry high content, sugar beet molasses, are unsuited for processes Ustilaginaceae, they result low product yields due to biomass formation. Results This study uncovers challenges evaluating complex feedstock applicability microbial processes, highlighting role secondary limitations, internal storage molecules, incomplete assimilation these substrates. microliter-scale screening method online monitoring respiration was developed using Ustilago trichophora on molasses application example. Investigation into nitrogen, phosphate, sulphate, magnesium limitations defined minimal medium demonstrated successful phosphate Furthermore, reduction elemental composition U. revealed respective These adaptive changes combination intricate metabolic response hinder mathematical prediction formation make presented methodology imperative. In next step, transferred molasses-based medium. It determined that organism assimilated only 25% 50% phosphorus present respectively. Due overall content bioavailable replacement state-of-the-art limitation shown increase by 65%. Conclusion The identification superior enhanced opens up new opportunities effective utilization more sustainable chemical

Language: Английский

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Development and characterization of acidic-pH-tolerant mutants of Zymomonas mobilis through adaptation and next-generation sequencing-based genome resequencing and RNA-Seq

Biotechnology for Biofuels, Journal Year: 2020, Volume and Issue: 13(1)

Published: Aug. 13, 2020

Acid pretreatment is a common strategy used to break down the hemicellulose component of lignocellulosic biomass release pentoses, and subsequent enzymatic hydrolysis step usually applied hexoses from cellulose. The hydrolysate after containing both pentoses can then be as substrates for biochemical production. However, acid-pretreated liquor also directly substrate microbial fermentation, which has an acidic pH contains inhibitory compounds generated during pretreatment. Although natural ethanologenic bacterium Zymomonas mobilis grow in broad range 3.5 ~ 7.5, cell growth ethanol fermentation are still affected under acidic-pH conditions below 4.0.In this study, adaptive laboratory evolution (ALE) was adapt Z. conditions. Two mutant strains named 3.6M 3.5M with enhanced tolerance were selected confirmed, grew better than ZM4 but worse that served reference strain between help unravel mechanism. Mutant exhibited 50 130% enhancement on rate, 4 9 h reduction time consume glucose, 20 63% improvement productivity wild-type at 3.8. Next-generation sequencing (NGS)-based whole-genome resequencing (WGR) RNA-Seq technologies mechanism strains. WGR result indicated compared ZM4, have seven five single nucleotide polymorphisms (SNPs), respectively, among four shared common. Additionally, showed upregulation genes involved glycolysis downregulation flagellar mobility related would generate redistribute cellular energy resist while keeping normal biological processes mobilis. Moreover, RND efflux pump, ATP-binding cassette (ABC) transporter, proton consumption, alkaline metabolite production significantly upregulated mutants condition could maintain homeostasis resistance. Furthermore, our results demonstrated 3.6M, encoding F1F0 ATPase pump excess protons out cells 3.8 6.2. This difference might manage 3.5M. A few gene targets genetics study explore their role tolerance, expression two operons shuttle plasmids, ZMO0956-ZMO0958 cytochrome bc1 complex ZMO1428-ZMO1432 tolerate conditions.An acidic-pH-tolerant obtained through commercial bioethanol In addition, molecular further proposed, facilitate future research rational design synthetic microorganisms against developed combining approaches ALE, genome resequencing, RNA-Seq, classical characterization other industrial microorganisms.

Language: Английский

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Polyphosphate fertilizer use efficiency strongly relies on soil physicochemical properties and root-microbial activities

Geoderma, Journal Year: 2022, Volume and Issue: 429, P. 116281 - 116281

Published: Nov. 26, 2022

Limited phosphorus (P) bioavailability restricts global agriculture and food production. This element is considered the least plant-available nutrient, it highly susceptible to immobilization in soil matrix. Among mineral fertilizers used increase P fertility, polyphosphates (PolyP) consist of polymers OrthoP residues have been shown improve crop uptake resulting yields more than other forms amendments. PolyP are also known for their progressive hydrolysis, improving availability rhizosphere plant throughout growth stages. However, behavior soils still understudied, including traits likely be involved use efficiency within soil-root-microbe interface. To our knowledge behavioral properties soil–plant continuum, this review among first studies compile, discuss, propose ideas regarding research while focusing on key biochemical factors responsible hydrolysis by roots. A combination exuded P-hydrolyzing enzymes acidification can presumably mobilize available from thereby acquisition. The importance root-associated microbes (exhibiting high P-mobilization capacities) discussed as a promising trait that could contribute greatly boost thus agronomic efficiency.

Language: Английский

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Peptide Antibiotic–Polyphosphate Nanoparticles: A Promising Strategy to Overcome the Enzymatic and Mucus Barrier of the Intestine

Biomacromolecules, Journal Year: 2023, Volume and Issue: 24(6), P. 2587 - 2595

Published: May 24, 2023

The aim of this study was to develop peptide antibiotic-polyphosphate nanoparticles that are able overcome the enzymatic and mucus barriers providing a targeted drug release directly on intestinal epithelium. Polymyxin B-polyphosphate (PMB-PP NPs) were formed via ionic gelation between cationic anionic polyphosphate (PP). resulting NPs characterized by particle size, polydispersity index (PDI), zeta potential, cytotoxicity Caco-2 cells. protective effect these for incorporated PMB evaluated degradation studies with lipase. Moreover, diffusion investigated porcine mucus. Isolated alkaline phosphatase (IAP) employed trigger consequent release. PMB-PP exhibited an average size 197.13 ± 14.13 nm, PDI 0.36, potential -11.1 3.4 mV concentration time-dependent toxicity. They provided entire protection toward significantly (p < 0.05) higher permeating properties than PMB. When incubated isolated IAP 4 h, monophosphate constantly released from raised up -1.9 0.61 mV. According findings, promising delivery systems protect antibiotics against degradation, barrier provide at

Language: Английский

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Spectroscopically dark phosphate features revealed by chemical exchange saturation transfer

NMR in Biomedicine, Journal Year: 2023, Volume and Issue: 37(2)

Published: Oct. 18, 2023

Abstract Phosphate is an essential anion in the human body, comprising approximately 1% of total body weight, and playing a vital role metabolism, cell membranes, bone formation. We have recently provided spectroscopic, microscopic, computational evidence indicating that phosphates can aggregate much more readily solution than previously thought. This prior work indirect through observation unusual P NMR relaxation line‐broadening effects with increasing temperature. Here, we show that, under conditions slow exchange selective RF saturation, additional features become visible chemical saturation transfer (CEST) experiments, which appear to be related reported phosphate clustering. In particular, CEST shows pronounced dips several ppm upfield main resonance at low temperatures, while direct spectroscopy does not produce any signals range. study pH dependence these new spectroscopic present parameters based on fitting data. These findings could importance investigation dynamics, especially biological milieu.

Language: Английский

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