Towards a more labor-saving way in microbial ammonium oxidation: A review on complete ammonia oxidization (comammox)

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 829, P. 154590 - 154590

Published: March 17, 2022

Language: Английский

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Genomic profiling of Nitrospira species reveals ecological success of comammox Nitrospira

Microbiome, Journal Year: 2022, Volume and Issue: 10(1)

Published: Nov. 30, 2022

Abstract Background The discovery of microorganisms capable complete ammonia oxidation to nitrate (comammox) has prompted a paradigm shift in our understanding nitrification, an essential process N cycling, hitherto considered require both oxidizing and nitrite microorganisms. This intriguing metabolism is unique the genus Nitrospira , diverse taxon previously known only contain canonical oxidizers. Comammox have been detected environments; however, global view distribution, abundance, diversity species still incomplete. Results In this study, we retrieved 55 metagenome-assembled genomes (MAGs) from newly obtained publicly available metagenomes. Combined with MAGs, constitutes largest genome database date 205 representing 132 putative species, most without cultivated representatives. Mapping metagenomic sequencing reads various environments against enabled analysis distribution habitat preferences species. ’s ecological success evident as they outnumber present higher species-level richness than all examined, except for marine wastewaters samples. type environment governs large-scale biogeographical signal. We found that closely related tend occupy same habitats, phylogenetic signal preference stronger . eco-evolutionary history more complex, subclades achieving rapid niche divergence via horizontal transfer genes, including gene encoding hydroxylamine oxidoreductase, key enzyme nitrification. Conclusions Our study expands genomic inventory genus, exposes oxidizers within wide range identifies (sub)lineages comammox proposes genes involved nitrification linked separation sublineage Nitrospira.

Language: Английский

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Chlorate as a comammox Nitrospira specific inhibitor reveals nitrification and N2O production activity in coastal wetland

Soil Biology and Biochemistry, Journal Year: 2022, Volume and Issue: 173, P. 108782 - 108782

Published: July 30, 2022

Language: Английский

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Co-Occurrence and Cooperation between Comammox and Anammox Bacteria in a Full-Scale Attached Growth Municipal Wastewater Treatment Process

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(12), P. 5013 - 5023

Published: March 13, 2023

Cooperation between comammox and anammox bacteria for nitrogen removal has been recently reported in laboratory-scale systems, including synthetic community constructs; however, there are no reports of full-scale municipal wastewater treatment systems with such cooperation. Here, we report intrinsic extant kinetics as well genome-resolved characterization a integrated fixed film activated sludge (IFAS) system where co-occur appear to drive loss. Intrinsic batch kinetic assays indicated that majority the aerobic ammonia oxidation was driven by (1.75 ± 0.08 mg-N/g TS-h) attached growth phase, minimal contribution ammonia-oxidizing bacteria. Interestingly, portion total inorganic (∼8%) consistently lost during these assays. Aerobic nitrite eliminated possibility denitrification cause loss, while anaerobic resulted rates consistent stoichiometry. Full-scale experiments at different dissolved oxygen (DO = 2 - 6 mg/L) setpoints persistent loss partly sensitive DO concentrations. Genome-resolved metagenomics confirmed high abundance (relative 6.53 0.34%) two Brocadia-like populations, within Ca. Nitrospira nitrosa cluster were lower (0.37 0.03%) Nitrosomonas-like oxidizers even (0.12 0.02%). Collectively, our study first time co-occurrence cooperation system.

Language: Английский

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Complete ammonia oxidization in agricultural soils: High ammonia fertilizer loss but low N₂O production

Global Change Biology, Journal Year: 2023, Volume and Issue: 29(7), P. 1984 - 1997

Published: Jan. 6, 2023

The contribution of agriculture to the sustainable development goals requires climate-smart and profitable farm innovations. Increasing ammonia fertilizer applications meet global food demands results in high agricultural costs, environmental quality deterioration, warming, without a significant increase crop yield. Here, we reported that third microbial oxidation process, complete (comammox), is contributing loss (41.9 ± 4.8%) at rate 3.53 0.55 mg N kg-1 day-1 soils around world. comammox loss, occurring mainly surface soil profiles (0-0.2 m), was equivalent bacterial (48.6 4.5%); both processes were significantly more important than archaeal (9.5 3.6%). In contrast, produced less N2 O (0.98 0.44 μg , 11.7 3.1%), comparable by (16.4 4.4%) but lower (72.0 5.1%). efficiency conversion (0.02 0.01%) evidently (0.24 0.06%) (0.16 0.04%) oxidation. increased with increasing pH values, which only physicochemical characteristic influenced abundance rates. Ammonia dominated oxidation, intense >6.5 <6.5. Our revealed plays vital role agroecosystems have been previously overlooked for long term.

Language: Английский

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Biodegradable microplastics boost dissimilatory nitrate reduction to ammonium (DNRA) process contributing to ammonium nitrogen retention in farmland soils

Journal of Cleaner Production, Journal Year: 2024, Volume and Issue: 438, P. 140835 - 140835

Published: Jan. 1, 2024

Language: Английский

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Metabolic Profiles and Microbial Synergy Mechanism of Anammox Biomass Enrichment and Membrane Fouling Alleviation in the Anammox Dynamic Membrane Bioreactor

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(14), P. 6284 - 6295

Published: March 15, 2024

The anammox dynamic membrane bioreactor (DMBR) is promising in applications with enhanced biomass enrichment and fouling alleviation. However, the metabolic mechanism underlying functional features of sludge biofilm still obscure. We investigated networks DMBR. cooperation between dissimilatory nitrate reduction to ammonium processes favored robust process rapid bacterial growth occurred DMBR 1.33 times higher yield compared MBR sludge, linked activities lipid metabolism, nucleotide B vitamin-related metabolism sludge. microbial community benefited alleviation that abundant fermentative bacteria their promoted organics degradation. intensified degradation foulants by was further evidenced active carbohydrate upregulated vitamin intermediates biofilms Our findings provide insights into key mechanisms for control DMBR, guiding manipulations overcoming loss treatment wastewater under detrimental environmental conditions.

Language: Английский

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Nitrifying niche in estuaries is expanded by the plastisphere

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 12, 2024

The estuarine plastisphere, a novel ecological habitat in the Anthropocene, has garnered global concerns. Recent geochemical evidence pointed out its potential role influencing nitrogen biogeochemistry. However, biogeochemical significance of plastisphere and mechanisms regulating cycling remain elusive. Using

Language: Английский

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Comammox activity dominates nitrification process in the sediments of plateau wetland

Water Research, Journal Year: 2021, Volume and Issue: 206, P. 117774 - 117774

Published: Oct. 18, 2021

Language: Английский

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Comammox Nitrospira play a minor role in N2O emissions from an alkaline arable soil

Soil Biology and Biochemistry, Journal Year: 2022, Volume and Issue: 171, P. 108720 - 108720

Published: May 21, 2022

Language: Английский

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