Environmental Science & Technology,
Journal Year:
2023,
Volume and Issue:
57(51), P. 21503 - 21526
Published: Dec. 14, 2023
Innovation
in
decarbonizing
wastewater
treatment
is
urgent
response
to
global
climate
change.
The
practical
implementation
of
anaerobic
ammonium
oxidation
(anammox)
treating
domestic
the
key
reconciling
carbon-neutral
management
with
sustainable
development.
Nitrite
availability
prerequisite
anammox
reaction,
but
how
achieve
robust
nitrite
supply
and
accumulation
for
mainstream
systems
remains
elusive.
This
work
presents
a
state-of-the-art
review
on
recent
advances
anammox,
paying
special
attention
available
pathways
(forward-going
(from
nitrite)
backward-going
nitrate
nitrite)),
controlling
strategies,
physiological
ecological
characteristics
functional
microorganisms
involved
supply.
First,
we
comprehensively
assessed
nitrite-oxidizing
bacteria
control
methods,
outlining
that
these
technologies
are
transitioning
possessing
multiple
selective
pressures
(such
as
intermittent
aeration
membrane-aerated
biological
reactor),
integrating
side
stream
free
ammonia/free
nitrous
acid
suppression
recirculated
sludge
treatment),
maintaining
high
activity
ammonia-oxidizing
competing
oxygen
bacteria.
We
then
highlight
emerging
strategies
supply,
including
production
driven
by
novel
microbes
(ammonia-oxidizing
archaea
complete
ammonia
bacteria)
reduction
(partial
denitrification
nitrate-dependent
methane
oxidation).
resources
requirement
different
analyzed,
hybrid
pathway
combining
partial
nitrification
encouraged.
Moreover,
data-driven
modeling
process
well
proactive
microbiome
proposed
hope
achieving
application.
Finally,
existing
challenges
further
perspectives
highlighted,
i.e.,
investigation
nitrite-supplying
bacteria,
scaling-up
from
laboratory
under
real
conditions,
stable
performance
fundamental
insights
this
aim
inspire
advance
our
understanding
about
provide
robustly
shed
light
important
obstacles
warranting
settlement.
Environmental Science & Technology,
Journal Year:
2024,
Volume and Issue:
58(23), P. 10140 - 10148
Published: May 23, 2024
Anammox
bacteria
performed
the
reaction
of
NH4+
and
NO
with
hydrazine
synthase
to
produce
N2H4,
followed
by
decomposition
N2H4
dehydrogenase
generate
N2.
Ferroheme/ferriheme,
which
serves
as
active
center
both
dehydrogenase,
is
thought
play
a
crucial
role
in
synthesis
during
due
its
high
redox
activity.
However,
this
has
yet
be
proven
exact
mechanisms
ferroheme/ferriheme
involved
process
remain
unclear.
In
study,
abiotic
biological
assays
confirmed
that
ferroheme
participated
reactions
ferriheme,
produced
reacted
ferriheme
N2
ferroheme.
other
words,
cycle
drove
continuous
between
NO.
Raman,
ultraviolet–visible
spectroscopy,
X-ray
absorption
fine
structure
spectroscopy
via
core
FeII/FeIII
cycle.
The
mechanism
participation
was
proposed
density
functional
theory
calculations.
These
findings
revealed
for
first
time
heme
electron
transfer
mechanisms,
are
great
significance
deepening
understanding
Anammox.
Sulfate-reducing
bacteria
(SRB)
are
anaerobic
microorganisms
that
use
sulfate
as
a
terminal
electron
acceptor
for
the
oxidation
of
organic
compounds
or
H2.
These
organisms
can
cause
serious
problem
in,
example,
offshore
oil
industry,
due
to
production
sulfide.
Thus,
it
is
fundamental
and
practical
importance
identify
potent
selective
inhibitors
SRB.
In
this
study,
neutral
red
was
identified
previously
unrecognized
inhibitor
SRB,
with
several
orders
magnitude
higher
potency
than
most
commonly
used
industrial
biocides
inorganic
oxyanions.
Neutral
remained
SRB
growth
under
fermentative
conditions
tolerated
by
nitrate-reducing
bacteria.
After
30
days
exposure
14.2
μM
red,
sulfidogenesis
activity
SRB-enriched
biomass
reduced
98.3%,
abundance
populations
declined
from
25.5%
0.76%.
Transcriptomic
analysis
revealed
inhibition
central
reduction
pathway
implicated
in
mechanism
toxicity
against
growth.
Furthermore,
downregulation
two
transport
complexes
(QmoABC
DsrMKJOP),
ATP
synthase,
well
cytoplasmic/periplasmic
hydrogenase
suggested
collapse
proton
gradient.
findings
have
implications
environmental
control
may
enhance
economic
benefits
operations.
