Published: Jan. 1, 2024

2, 6-di-tert-butyl-p-cresol (BHT) is a synthetic phenolic antioxidant and pose potential threat to aquatic organisms. However, as the emerging contaminant, little known about response of microalgae BHT. In this study, BHT significantly inhibited growth photosynthetic pigment content Phaeodactylum tricornutum with increase concentration. The exposure also led an in malondialdehyde algal cells, indicating oxidative stress caused by Simultaneously, responded environment enhancing activities three enzymes, superoxide dismutase, catalase peroxidase eliminate excess reactive oxygen species cells during various time. Furthermore, transcriptome analysis revealed that up-regulation genes related photosynthesis, TCA cycle, phosphorylation indole-3-acetic acid (IAA) stress. addition, our high-throughput Illumina MiSeq sequencing results demonstrated significant relative abundance BHT-tolerant bacteria affiliated Halomonas, which was helpful reduce on P. tricornutum. Microbiological assays further Halomonas can thrive using IAA sole energy source exhibit chemotactic IAA. Therefore, we conclude phycosphere promotes colonization or recruitment thereby adaptability environmental pressures. Overall, study provides comprehensive understanding physiological biochemical effects microalgae, highlight functional significance establishing interaction between algae-associated adverse environments.

Language: Английский