A Function-Driven Single-Cell Approach to Unveiling and Cultivating Hidden Microplastic-Degrading Microbes from Insect Gut Microbiota DOI
Cui Li, Hongqin Guo, Kai Yang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Abstract Biodegradation is a sustainable strategy to address global microplastics (MPs) pollution but constrained by the lack of efficient degrading microbes and effective tools harness them. Here, we developed function-driven single-cell approach precisely identify recover MPs-degrading microorganisms from complex microbiota integrating isotope-labeled Raman spectroscopy with targeted cell sorting, sequencing culturing. Using heavy water MPs as sole carbon source, effectively identified active capable multiple types insect gut microbiota. Raman-guided sorting revealed seven previously unrecognized polystyrene degraders mapped key enzymes involved in each degradation stage. Furthermore, live-cell enabled cultivation rare highly often missed conventional methods. This “screen-first, culture-second” offers powerful scalable platform accelerate biodegradation supports development microbial solutions mitigate plastic pollution.

Language: Английский

Freeze-thaw aged polyethylene and polypropylene microplastics alter enzyme activity and microbial community composition in soil DOI
Yanjun Li, Guanghui Xu, Yong Yu

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 470, P. 134249 - 134249

Published: April 9, 2024

Language: Английский

Citations

14
Comparison of sulfide-induced transformation of biodegradable and conventional microplastics: Mechanism and environmental fate DOI
Tingting Du,

Liwen Qian,

Song Shao

et al.

Water Research, Journal Year: 2024, Volume and Issue: 253, P. 121295 - 121295

Published: Feb. 8, 2024

Language: Английский

Citations

11
Biochar immobilized hydrolase degrades PET microplastics and alleviates the disturbance of soil microbial function via modulating nitrogen and phosphorus cycles DOI
Huawen Han, Peizhi Song,

Yuchao Jiang

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 474, P. 134838 - 134838

Published: June 5, 2024

Language: Английский

Citations

11
A novel Gordonia sp. PS3 isolated from the gut of Galleria mellonella larvae: mechanism of polystyrene biodegradation and environmental toxicological evaluation DOI

Luhui Xu,

Xuejiao An,

Huoyong Jiang

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 137219 - 137219

Published: Jan. 1, 2025

Language: Английский

Citations

1
Microplastics in freshwater and marine ecosystems: Occurrence, characterization, sources, distribution dynamics, fate, transport processes, potential mitigation strategies, and policy interventions DOI
Toheeb Lekan Jolaosho,

Mariam Folashade Rasaq,

Eniola Victoria Omotoye

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 294, P. 118036 - 118036

Published: March 18, 2025

Language: Английский

Citations

1
Biodegradation of low-density polyethylene film by two bacteria isolated from plastic debris in coastal beach DOI Creative Commons
Zhen Rong, Xue‐Wei Xu, Yue‐Hong Wu

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 278, P. 116445 - 116445

Published: May 10, 2024

Low-density polyethylene (LDPE) conduces massive environmental accumulation due to its high production and recalcitrance environment. In this study, We successfully enriched isolated two strains, Nitratireductor sp. Z-1 Gordonia Z-2, from coastal plastic debris capable of degrading LDPE film. After a 30-day incubation at 30 ℃, strains Z-2 decreased the weight branched-LDPE (BLDPE) film by 2.59 % 10.27 respectively. Furthermore, temperature gel permeation chromatography (HT-GPC) analysis revealed molecular reductions 7.69 (Z-1) 23.22 (Z-2) in BLDPE Scanning electron microscope (SEM) image showed presence microbial colonization perforations on film's surface. Fourier transform infrared spectroscopy (FTIR) indicated novel functional groups, such as carbonyl carbon-carbon double bonds films. During degradation, both produced extracellular reactive oxygen species (ROS). GC-MS degradation products included short-chain alkanes, alkanols, fatty acids, esters. Genomic identified numerous enzymes potentially involved chain scission. A model was proposed suggesting coordinated role between ROS biodegradation LDPE. This indicates can degrade LDPE, providing basis for deeper exploration mechanisms.

Language: Английский

Citations

7
Recent trends in microbial and enzymatic plastic degradation: a solution for plastic pollution predicaments DOI Creative Commons

Swagata Lakshmi Dhali,

Dinesh Parida, Bikash Kumar

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(1)

Published: Aug. 11, 2024

Plastic pollution is an ever-escalating issue with detrimental effects on both the environment and human health. breaks down into smaller pieces, depending size they are called macroplastics, microplastics (MPs), nanoplastics (NPs). Some of these particles can easily enter food chain causing toxicity to many plants animals. The extensive use synthetic polymers such as polyethylene (PE), polyvinyl chloride (PVC), polystyrene (PS), terephthalate (PET) poses substantial environmental concerns due their degradation-resistant characteristics. One ways microorganisms address this by producing enzymes. This review examines recent advancements in enzymatic degradation commercial-grade pure polymers, including effectiveness enzymes laccases, proteases, cutinases, PETase, MHETase, governing mechanisms across various plastic categories. Bioinformatic tools multi-omics, molecular docking, enzyme mining particularly useful identifying unconventional biocatalysts plastic-degrading microbes a culture-independent manner. Furthermore, techniques enhance catalytic efficiency degrading (PDEs) using modern approaches protein engineering, mutations, chimeric fusion, etc. have also been reviewed. accentuates pivotal role microbial mitigating pollution, associated challenges, suitable prospects achieve closed-loop recycling future.

Language: Английский

Citations

6
Microbial consortia for multi-plastic waste biodegradation: selection and validation DOI Creative Commons
Jesús Salinas,

M.R. Martínez-Gallardo,

M.M. Jurado

et al.

Environmental Technology & Innovation, Journal Year: 2024, Volume and Issue: 36, P. 103887 - 103887

Published: Nov. 1, 2024

Language: Английский

Citations

6
“Metagenomic analysis of soil from landfill site reveals a diverse microbial community involved in plastic degradation” DOI
Akhilesh Kumar, Sudarshan Singh Lakhawat, Kashmir Singh

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 135804 - 135804

Published: Sept. 10, 2024

Language: Английский

Citations

5
Supercritical Co2 -Promoted Degradation of Polystyrene to Aromatic Oils with Nio@C Catalyst DOI
Yanbing Liu, Jinwen Shi,

Zitong Zhuang

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

4