Gene Modification and Omics Technology for Pollution Management

Published: Jan. 1, 2025

Language: Английский

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Unveiling organohalide respiration potential in River Nile sediments via 16S rRNA gene amplicon sequencing of endogenous bacterial communities

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 31, 2025

Abstract Background Industrial waste, agricultural runoff and untreated sewage contaminate the Nile, leaving a toxic legacy in its sediments. Organohalides-polluted sediment particular poses serious public health risks detrimental effects on aquatic life. Sediment microbiomes may harbor bacterial strains that could be utilized bioremediation of such pollutants. Material methods Two from polluted River Nile sediments were analyzed by using 16S rRNA gene amplicon sequencing. In addition, PICRUSt analysis based data was used to explore organohalide respiring bacteria (OHRB) genera their corresponding respiration (OHR) activity. Microcosm studies performed validate potential for dechlorination activity sediment. Dechlorination parent chloroethenes into daughter end product detected gas chromatography coupled with flame ionization detection analysis. Results Analysis sequences EZ-biocloud server identified Proteobacteria as dominant phylum both microbiomes, Bacteroidetes Chloroflexi prevalent RNS1 Chlorobi RNS2 PCR analyses several OHRB genera, including Dehalococcoides , Dehalogenimonas Desulfomonile Desulfovibrio Geobacter suggesting OHR Further evidence provided functional prediction analysis, which suggested presence reductive dehalogenases biomarkers associated samples. Specifically, predicted genes tetrachloroethene dehalogenase 3-chloro-4-hydroxyphenylacetate dehalogenase, previously linked OHR. confirmed dichloroethene. Conclusion This study demonstrates industrialized area harbors distinct enclosing various providing substantial It also provides

Language: Английский

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Geo-Microbes in Rhizoremediation: Harnessing Potential of Sulphate-Reducing Bacteria for Mitigation of Xenobiotics Compounds

Published: Jan. 1, 2025

Language: Английский

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Advancing crop resilience through nucleic acid innovations: rhizosphere engineering for food security and climate adaptation

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143194 - 143194

Published: April 1, 2025

Language: Английский

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Revealing the intricate temporal dynamics and adaptive responses of prokaryotic and eukaryotic microbes in the coastal South China Sea

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 952, P. 176019 - 176019

Published: Sept. 3, 2024

Language: Английский

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The Vestfold Hills are alive: characterising microbial and environmental dynamics in Old Wallow, eastern Antarctica

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 23, 2024

Old Wallow is an underexplored, hyper-arid coastal desert in Antarctica’s Vestfold Hills. Situated near elephant seal wallow, we examined how stochastic nutrient inputs from the wallow affect soil communities amid environmental changes along a spatially explicit sampling transect. We hypothesized that levels would be elevated due to proximity influencing community distributions. While bacterial and eukaryotic at phylum level were similar other terrestrial environments, analysis class family revealed dominance of unclassified taxa are often linked marine environments. Elevated concentrations (NO 3 − , SO 4 2− ) found Wallow, with conductivity Cl up 10-fold higher lowest elevation soils, correlating significantly ( p < 0.05) abundances halophilic Halomonadaceace uncultivated lineages Ca Actinomarinales, Bacillariophyta Opisthonkonta ). An improved Gradient Forest model was used quantify microbial responses 26 gradients OW, revealing variable predictors identifying critical thresholds or drivers turnover. Major tipping points projected for eukaryotes pH, bacteria moisture, Na 2 O, . Thus, ecosystem primarily shaped by salt, sulphate, moisture dominated taxa, which may sensitive once reached. This study provides baseline data future regional monitoring under threats change.

Language: Английский

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Use of qPCR to Monitor 2,4-Dinitroanisole Degrading Bacteria in Water and Soil Slurry Cultures

Journal of Industrial Microbiology & Biotechnology, Journal Year: 2024, Volume and Issue: 51

Published: Jan. 1, 2024

Language: Английский

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Organohalide respiration: retrospective and perspective through bibliometrics

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 24, 2024

Organohalide-respiring bacteria (OHRB) play a pivotal role in the transformation of organohalogens diverse environments. This bibliometric analysis provides timely overview OHRB research trends and identifies knowledge gaps. Publication numbers have steadily increased since process was discovered 1982, with fluctuations total citations average per publication. The past decade witnessed peak publications, underscoring heightened activity extensive collaboration. Thematic identified two primary foci: mechanistic exploration their interplay environmental factors. Future should prioritize elucidating roles OHRB’s biogeochemical cycling, utilizing synthetic biology tools for enhanced biotransformation, deciphering ecological interactions, unraveling evolutionary pathways, investigating dehalogenation capabilities other microorganisms, including archaea. These directions promise to advance our understanding microbially-driven organohalide transformations, microbial ecology, genetic engineering potential, ultimately informing natural cycling management strategies.

Language: Английский

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Integrated use of electrochemical anaerobic reactors and genomic based modeling for characterizing methanogenic activity in microbial communities exposed to BTEX contamination

Environmental Research, Journal Year: 2024, Volume and Issue: unknown, P. 120691 - 120691

Published: Dec. 1, 2024

Language: Английский

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