Visible light driven Z-scheme α-MnO2 (1D)/Bi7O9I3 (2D) heterojunction photocatalyst for efficient degradation of bisphenol A in water

Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(3), P. 112879 - 112879

Published: April 23, 2024

Language: Английский

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Transcriptomic response of an algal species (raphidocelis subcapitata) exposed to 22 per- and polyfluoroalkyl substances

Environmental Toxicology and Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

Per- and polyfluoroalkyl substances (PFAS) are a large class of chemicals concern for both human environmental health because their ubiquitous presence in the environment, persistence, potential toxicological effects. Despite this, ecological hazard data limited to small number PFAS even though there over 4000 identified PFAS. Traditional toxicity testing will likely be inadequate generate necessary information risk assessment. Therefore, present study investigated utility using transcriptomic points departure (tPODs) informing algal toxicity. Raphidocelis subcapitata, freshwater green species, were exposed 24 hours 96 well microplates multiple concentrations 22 different Following exposure, RNA was extracted, transcriptome evaluated by sequencing followed concentration response modeling determine tPOD each tPODs, based upon measured concentrations, ranged from 0.9 µg/L perfluorotridecanoic acid 1 mg/L perfluorononanoic acid. These values derived R. subcapitata exposures compared published benchmarks other taxa (larval fathead minnow Daphnia magna) vitro data. While generally more sensitive tested than previously assays, tPODs were, on average, three orders magnitude greater maximum detected Great Lakes tributaries. This high throughput transcriptomics assay with algae is promising new approach method an ecologically relevant, tiered evaluation strategy.

Language: Английский

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TempO-seq and RNA-seq gene expression levels are highly correlated for most genes: A comparison using 39 human cell lines

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(5), P. e0320862 - e0320862

Published: May 9, 2025

Recent advances in transcriptomics technologies allow for whole transcriptome gene expression profiling using targeted sequencing techniques, which is becoming increasingly popular due to logistical ease of data acquisition and analysis. As from these platforms accumulates, it important evaluate their similarity traditional RNA-seq. Thus, we evaluated the comparability TempO-seq cell lysates RNA-Seq purified RNA baseline profiles. First, two sets that were generated several months apart at different read depths compared six human lines. The average Pearson correlation was 0.93 (95% CI: 0.90–0.96) principal component analysis (PCA) showed highly reproducible could be combined. Next, 39 log 2 normalized 19,290 genes within both well correlated between RNA-seq (Pearson 0.77, 95% 0.76–0.78), majority (15,480 genes, 80%) had concordant levels. PCA a platform divergence, but this readily resolved by calculating relative (RLE) across lines each platform. Application ontology revealed ontologies associated with histone ribosomal functions enriched 20% non-concordant levels (3,810 genes). On other hand, annotated cellular structure platforms. In conclusion, found RLE lysed cells comparable lines, even though datasets laboratories stocks.

Language: Английский

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Community‐Engaged Research and the Use of Open Access ToxVal/ToxRef In Vivo Databases and New Approach Methodologies (NAM) to Address Human Health Risks From Environmental Contaminants

Birth Defects Research, Journal Year: 2024, Volume and Issue: 116(9)

Published: Sept. 1, 2024

ABSTRACT Background The paper analyzes opportunities for integrating Open access resources (Abstract Sifter, US EPA and NTP Toxicity Value Reference [ToxVal/ToxRefDB]) New Approach Methodologies (NAM) integration into Community Engaged Research (CEnR). Methods CompTox Chemicals Dashboard Integrated Chemical Environment with in vivo ToxVal/ToxRef NAMs (in vitro) databases are presented three case studies to show how these could be used Pilot Projects involving (CEnR) from the University of California, Davis, Environmental Health Sciences Center. Results Case #1 developed a novel assay methodology testing pesticide toxicity. #2 involved detection water contaminants wildfire ash #3 on Tribal Lands. Abstract Sifter/ToxVal/ToxRefDB regulatory data screen/prioritize risks exposure metals, PAHs PFAS leached investigate activities environmental toxins (e.g., pesticides) lands. computational tools can apply sensitive biological potential or known adverse outcome pathways predict points departure (POD) comparison values hazard identification. Systematic Empirical Evaluation Models biomonitoring exposures available human subpopulations determine bioactivity ratio facilitate mitigation. Conclusions These help prioritize chemical toxicity decisions health protective policies that aid stakeholders deciding needed research. Insights justice equity advocates.

Language: Английский

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