Construction of a synthetic anaerobic dechlorination microbiome to degrade chlorinated ethenes by application of metabolic interactions principle DOI

Zeyi Wang,

Yu Yang,

Rongjian Zhao

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 955, P. 176981 - 176981

Published: Oct. 18, 2024

Language: Английский

Underexplored Organohalide-Respiring Bacteria in Sewage Sludge Debrominating Polybrominated Diphenyl Ethers DOI
Guofang Xu, Siyan Zhao, Jianzhong He

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: July 20, 2024

Polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants prevalent in the environment. Organohalide-respiring bacteria (OHRB) can attenuate PBDEs via reductive debromination, but often producing toxic end-products. Debromination of to ether remains a rare phenomenon and is so far specifically associated with Dehalococcoides isolated from e-waste polluted sites. The occurrence PBDE debromination other ecosystems underpinning OHRB underexplored. Here we found that common trait sewage sludge microbiota, was produced as end-product at varying quantities (0.6-52.9% mol parent PBDEs) 76 84 cultures established bioreactor sludge. Diverse pathways converting ether, including several new routes, were identified. Although contributed Dehalogenimonas, Dehalobacter, uncultivated Dehalococcoidia likely played more important roles than previously recognized. Multiple dehalogenase genes (including bdeA, pcbA4, pteA, tceA) also coexisted Collectively, these findings contribute enhancing our comprehension environmental fate PBDEs, expanding diversity microorganisms catalyzing developing consortia for bioremediation application.

Language: Английский

Citations

5
Unravelling the processes involved in biodegradation of chlorinated organic pollutant: From microbial community to isolated organohalide degraders DOI
Meng Liu, Xin Su, Jing Yuan

et al.

Water Research, Journal Year: 2024, Volume and Issue: 268, P. 122730 - 122730

Published: Nov. 1, 2024

Language: Английский

Citations

3
How Pseudomonas conducts reductive dechlorination of 2,4,6-trichlorophenol: Insights into metabolic performance and organohalide respiration process DOI
Xueqi Chen, Zhiling Li, Zimeng Zhang

et al.

Water Research, Journal Year: 2024, Volume and Issue: 273, P. 123014 - 123014

Published: Dec. 19, 2024

Language: Английский

Citations

3
Influence of saline water and heavy rain on the fate of chlorinated ethenes in groundwater characterized by compound-specific isotope and microbial data DOI

Dugin Kaown,

Kenneth C. Carroll, Jürgen Mahlknecht

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 487, P. 137238 - 137238

Published: Jan. 15, 2025

Language: Английский

Citations

0
Plasmid Backbone Impacts Conjugation Rate, Transconjugant Fitness, and Community Assembly of Genetically Bioaugmented Soil Microbes for PAH Bioremediation DOI Creative Commons
Tessa Crosby, Lauren B. Stadler

ACS Environmental Au, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Many polycyclic aromatic hydrocarbons (PAHs) in the environment resulting from crude oil spills and incomplete combustion of organic matter are highly toxic, mutagenic, or carcinogenic to microorganisms humans. Bioremediation PAHs using that encode biodegradative genes is a promising approach for environmental PAH cleanup. However, viability exogenous often limited due competition with native microbial community. Instead relying on survival one few species bacteria, genetic bioaugmentation harnesses conjugative plasmids spread functional microbes. In this study, two plasmid backbones differ copy number regulation, replication, mobilization were engineered contain dioxygenase gene (bphC) conjugated soil bacteria including Bacillus subtilis, Pseudomonas putida, Acinetobacter sp., as well synthetic community assembled these bacteria. Fitness effects transconjugants significantly impacted rates transfer biotransformation model (2,3-dihydroxybiphenyl). A synergistic effect was observed which communities bioaugmented bphC had higher degradation than grown monocultures. Finally, conjugation associated relative abundances communities, underscoring how fitness impacts can shape structure function.

Language: Английский

Citations

0
Harnessing Sewage Sludge Microbiota from Wastewater Treatment Plants for Tetrachloroethene Detoxification DOI Creative Commons
Guofang Xu, Jianzhong He

ACS ES&T Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Language: Английский

Citations

0
Strategies and mechanisms for improving groundwater remediation efficiency of chlorinated ethenes by controlling the particle size of polyhydroxyalkanoate DOI
Jie Ma,

Linhui Lei,

Zhiwei Gong

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 496, P. 154038 - 154038

Published: July 15, 2024

Language: Английский

Citations

2
Construction of a synthetic anaerobic dechlorination microbiome to degrade chlorinated ethenes by application of metabolic interactions principle DOI

Zeyi Wang,

Yu Yang,

Rongjian Zhao

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 955, P. 176981 - 176981

Published: Oct. 18, 2024

Language: Английский

Citations

0