Dynamic non-coding RNA biomarker reveals lung injury and repair induced by polystyrene nanoplastics

Environment International, Journal Year: 2025, Volume and Issue: 195, P. 109266 - 109266

Published: Jan. 1, 2025

Polystyrene nanoplastics (PS-NPs) are omnipresent in the air and can be inhaled by humans. However, their long-term adverse implications toxicological mechanisms for human respiratory health unclear. Therefore, this study aims to provide new insights into pulmonary toxicity of PS-NPs using mice organoid models. After subacute subchronic inhalation PS-NPs, showed pronounced lung injury characterized rate changes, altered hematology, histological evidence tissue damage oxidative stress. Similarly, repeated exposure also restricted growth cause damage. Notably, through BisqueRNA analysis a single-cell dataset canonical markers verification, it was found that induced emergence accumulation transitional cells, suggesting impaired alveolar epithelial repair processes. Sequencing analyses revealed dynamic alterations non-coding RNA (ncRNA) profiles, including circRNAs lncRNAs, response exposure. Moreover, temporal profiling highlighted distinct sets ncRNAs as early progression-associated biomarkers PS-NP-induced injury. These correlated with aberrant implicating roles disrupted cellular differen tiation mechanisms. Overall, observed multifaceted responses system, emphasizing critical involvement mediating PS-NP-induce which crucial elucidating pathophysiology nanoplastic-induced developing targeted therapeutic strategies.

Language: Английский

In vitro cell-transforming capacity of micro- and nanoplastics derived from 3D-printing waste

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 293, P. 118007 - 118007

Published: March 1, 2025

The increasing use of plastic polymers in 3D printing applications may lead to human exposure micro- and nanoplastics (MNPLs), raising concerns regarding adverse health consequences such as cancer induction. Little attention has been given MNPLs originated at the end life cycle 3D-printed objects because mechanical environmental degradation waste. This study assessed carcinogenic potential secondary generated through cryomilling using validated vitro Bhas 42 cell transformation assay (CTA). Three-dimensional were printed four types polycarbonate (PC)- polypropylene (PP)-modified thermoplastic filaments, undoped doped with single-walled carbon nanotubes (PC-CNT) silver nanoparticles (PP-Ag), respectively. (< 5 µm) following a three-step top-down process thoroughly characterized. cells treated once (initiation assay) or repeatedly (promotion several concentrations (3.125-100 µg/mL) mimicking realistic conditions, transformed foci formation was evaluated after 21 days. Furthermore, cellular internalization mRNA expression seven genes previously recognized part predictive early signature also evaluated. Despite being internalized, none particles able initiate promote transformation, regardless doping nanomaterials. Alternatively, all significantly increased decreased Prl2c3 Timp4, respectively, under promotion indicating changes that occur before foci. These findings suggest test could have tumorigenic despite not showing morphological cells.

Language: Английский

The released micro/nano-plastics from plastic containers amplified the toxic response of disinfection by-products in human cells

Food Chemistry, Journal Year: 2024, Volume and Issue: 470, P. 142636 - 142636

Published: Dec. 24, 2024

Language: Английский