Lysogen formation governs colonies while lytic infection is more prevalent in single cells of the bloom-forming cyanobacterium,Microcystis DOI Creative Commons
Xuhui Huang, Emily E. Chase, Brittany N. Zepernick

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

While the bloom-forming cyanobacterium Microcystis can exist as free-living single cells or within dense mucilaginous colonies, drivers and consequences of colony formation remain unclear. Here, we integrated metatranscriptomic datasets from two bloom events in Lake Taihu, China, to analyze validate functional differences between colonial single-cell . Our results confirmed expression profiles were disproportionately enriched transcripts (and functions) compared other prokaryotic taxa. Concomitantly, viral infection strategies diverged by community morphology: colony-associated expressed lysogeny-associated genes, while exhibited increased signatures lytic infection. These data are consistent with hypothesis that colonies foster conditions favorable lysogen formation-likely due local high cell densities resulting advantage superinfection immunity-whereas solitary experience stronger pressure. On a broader scale, our findings refine understanding dynamics identifying how morphological states coincide distinct host-virus interactions. Cumulatively, this work underscores importance shaping ecology highlights need for mechanistic studies disentangle interplay phage modes, formation, microbial structure.

Language: Английский

Potassium permanganate as a promising pre-oxidant to treat low-viability cyanobacteria and associated removal of cyanotoxins and extracellular organic matters DOI
Xi Li, Jie Zeng, Xin Yu

et al.

Water Research, Journal Year: 2021, Volume and Issue: 202, P. 117353 - 117353

Published: June 10, 2021

Language: Английский

Citations

28
Shift of calcium-induced Microcystis aeruginosa colony formation mechanism: From cell adhesion to cell division DOI
Xuhui Huang, Peng Gu,

Hanqi Wu

et al.

Environmental Pollution, Journal Year: 2022, Volume and Issue: 313, P. 119997 - 119997

Published: Aug. 19, 2022

Language: Английский

Citations

22
The extracellular polysaccharide determine the physico-chemical surface properties of Microcystis DOI Creative Commons
Haijian Yang,

Denghua Wu,

Hua Li

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 5, 2023

Microcystis possesses the capacity to form colonies and blooms in lakes reservoirs worldwide, causing significant ecological challenges aquatic ecosystems. However, little is known about determining factors of physico-chemical surface properties that govern competitive advantage Microcystis. Here, The wesenbergii aeruginosa, including specific area (SSA), hydrophobicity, zeta potential, functional groups were investigated. Additionally, extracellular polysaccharide (EPS) analyzed. Laboratory-cultured exhibited hydrophilic, a negative potential negatively charged. Furthermore, no relationship was shown between these cultivation stage. low free energy cohesion, high energy, growth rate, EPS content during logarithmic phase. On other hand, M. aeruginosa displayed lower content, rate stationary These characteristics contribute their respective advantage. component primarily influenced SSA total Likewise, protein hydrophobicity tension. composition, glucuronic acid, xylose, fructose, mainly properties. hydrophilic such as O-H P-O-P played crucial role This study elucidates SSA, cells, which turn impact formation collection.

Language: Английский

Citations

11
Production and release of 2-MIB in Pseudanabaena: Effects of growth phases on cell characteristics and 2-MIB yield DOI Creative Commons

Hailing Wang,

Lin Li,

Shaozhe Cheng

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 274, P. 116198 - 116198

Published: March 11, 2024

2-methylisoborneol (2-MIB), a secondary metabolite produced by cyanobacteria, often causes musty odour in water, threatening the safety of drinking water supplies. This study investigated effects growth phases on production 2-MIB Pseudanabaena. The cell characteristics and release were also explored. total concentration increased during exponential phase decreased declining phase, which was consistent with changes density. However, yield (1.12-1.27 fg

Language: Английский

Citations

4
Lysogen formation governs colonies while lytic infection is more prevalent in single cells of the bloom-forming cyanobacterium,Microcystis DOI Creative Commons
Xuhui Huang, Emily E. Chase, Brittany N. Zepernick

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

While the bloom-forming cyanobacterium Microcystis can exist as free-living single cells or within dense mucilaginous colonies, drivers and consequences of colony formation remain unclear. Here, we integrated metatranscriptomic datasets from two bloom events in Lake Taihu, China, to analyze validate functional differences between colonial single-cell . Our results confirmed expression profiles were disproportionately enriched transcripts (and functions) compared other prokaryotic taxa. Concomitantly, viral infection strategies diverged by community morphology: colony-associated expressed lysogeny-associated genes, while exhibited increased signatures lytic infection. These data are consistent with hypothesis that colonies foster conditions favorable lysogen formation-likely due local high cell densities resulting advantage superinfection immunity-whereas solitary experience stronger pressure. On a broader scale, our findings refine understanding dynamics identifying how morphological states coincide distinct host-virus interactions. Cumulatively, this work underscores importance shaping ecology highlights need for mechanistic studies disentangle interplay phage modes, formation, microbial structure.

Language: Английский

Citations

0