Environmental DNA gives comparable results to morphology-based indices of macroinvertebrates in a large-scale ecological assessment DOI Creative Commons
Jeanine Brantschen, Rosetta C. Blackman, Jean‐Claude Walser

et al.

PLoS ONE, Journal Year: 2021, Volume and Issue: 16(9), P. e0257510 - e0257510

Published: Sept. 21, 2021

Anthropogenic activities are changing the state of ecosystems worldwide, affecting community composition and often resulting in loss biodiversity. Rivers among most impacted ecosystems. Recording their current with regular biomonitoring is important to assess future trajectory Traditional monitoring methods for ecological assessments costly time-intensive. Here, we compared macroinvertebrates based on environmental DNA (eDNA) sampling traditional kick-net biodiversity patterns at 92 river sites covering all major Swiss catchments. From data, a biotic index (IBCH) 145 indicator taxa had been established. The was matched by taxonomically annotated eDNA data using machine learning approach. Our comparison diversity only uses zero-radius Operational Taxonomic Units assigned taxa. Overall, found strong congruence between both assessment total (gamma diversity). However, when assessing site level (alpha diversity), were less consistent gave complementary composition. Specifically, retrieved significantly fewer per than Importantly, however, subsequent classification rivers detected indicators resulted similar scores that classified random forest majority predictions (72%) from same status categories as Thus, validly communities and, combined learning, provided reliable classifications rivers. while gives macroinvertebrate approach, subsequently calculated indices nevertheless directly comparable consistent.

Language: Английский

Comparison of markers for the monitoring of freshwater benthic biodiversity through DNA metabarcoding DOI
Gentile Francesco Ficetola, Frédéric Boyer, Alice Valentini

et al.

Molecular Ecology, Journal Year: 2020, Volume and Issue: 30(13), P. 3189 - 3202

Published: Sept. 27, 2020

Abstract Metabarcoding of bulk or environmental DNA has great potential for biomonitoring freshwater environments. However, successful application metabarcoding to biodiversity monitoring requires universal primers with high taxonomic coverage that amplify highly variable, short metabarcodes resolution. Moreover, reliable and extensive reference databases are essential match the outcome analyses available taxonomy indices. Benthic invertebrates, particularly insects, key taxa bioassessment. Nevertheless, few studies have so far assessed markers macrobenthos. Here we combined in silico laboratory test performance different amplifying regions 18S rDNA (Euka02), 16S (Inse01) COI (BF1_BR2‐COI) genes, developed an database benthic macroinvertebrates France Europe, a particular focus on insect orders (Ephemeroptera, Plecoptera Trichoptera). Analyses 1,514 individuals representing showed very amplification success across primer combinations. The Euka02 marker highest universality, while Inse01 excellent insects. BF1_BR2‐COI resolution, resolution was often limited. By combining our data GenBank information, curated including sequences 822 genera. heterogeneous highlights complexity identifying best markers, advocates integration multiple more comprehensive accurate understanding ecological impacts biodiversity.

Language: Английский

Citations

54
eDNA biomonitoring revealed the ecological effects of water diversion projects between Yangtze River and Tai Lake DOI
Lijuan Zhang, Jianghua Yang, Yong Zhang

et al.

Water Research, Journal Year: 2021, Volume and Issue: 210, P. 117994 - 117994

Published: Dec. 22, 2021

Language: Английский

Citations

54
Toward Sustainable Environmental Quality: Priority Research Questions for Asia DOI Creative Commons
Kmy Leung,

Katie Wan Yee Yeung,

Jing You

et al.

Environmental Toxicology and Chemistry, Journal Year: 2020, Volume and Issue: 39(8), P. 1485 - 1505

Published: May 31, 2020

Environmental and human health challenges are pronounced in Asia, an exceptionally diverse complex region where influences of global megatrends extensive numerous stresses to environmental quality exist. Identifying priorities necessary engage grand can be facilitated through horizon scanning exercises, this end we identified examined 23 priority research questions needed advance toward more sustainable as part the Global Horizon Scanning Project. Advances toxicology, chemistry, biological monitoring, risk-assessment methodologies address adverse impacts stressors on ecosystem services biodiversity, with Asia being home biodiversity hotspots. Intersections food-energy-water nexus profound Asia; innovative aggressive technologies provide clean water, ensure food safety, stimulate energy efficiency, while improving ecological integrity addressing legacy emerging threats public environment, particularly increased aquaculture production. is largest chemical-producing continent globally. Accordingly, green chemistry engineering present decided opportunities innovation realize a number United Nations Sustainable Development Goals. Engaging herein will require transdisciplinary coordination existing nontraditional partnerships within among countries sectors. Answering these not easy but achieve Asia. Environ Toxicol Chem 2020;39:1485-1505. © 2020 The Authors. Toxicology Chemistry published by Wiley Periodicals LLC behalf SETAC.

Language: Английский

Citations

54
Bacterial community responses to tourism development in the Xixi National Wetland Park, China DOI
Binhao Wang, Xiafei Zheng, Hangjun Zhang

et al.

The Science of The Total Environment, Journal Year: 2020, Volume and Issue: 720, P. 137570 - 137570

Published: Feb. 25, 2020

Language: Английский

Citations

51
Environmental DNA gives comparable results to morphology-based indices of macroinvertebrates in a large-scale ecological assessment DOI Creative Commons
Jeanine Brantschen, Rosetta C. Blackman, Jean‐Claude Walser

et al.

PLoS ONE, Journal Year: 2021, Volume and Issue: 16(9), P. e0257510 - e0257510

Published: Sept. 21, 2021

Anthropogenic activities are changing the state of ecosystems worldwide, affecting community composition and often resulting in loss biodiversity. Rivers among most impacted ecosystems. Recording their current with regular biomonitoring is important to assess future trajectory Traditional monitoring methods for ecological assessments costly time-intensive. Here, we compared macroinvertebrates based on environmental DNA (eDNA) sampling traditional kick-net biodiversity patterns at 92 river sites covering all major Swiss catchments. From data, a biotic index (IBCH) 145 indicator taxa had been established. The was matched by taxonomically annotated eDNA data using machine learning approach. Our comparison diversity only uses zero-radius Operational Taxonomic Units assigned taxa. Overall, found strong congruence between both assessment total (gamma diversity). However, when assessing site level (alpha diversity), were less consistent gave complementary composition. Specifically, retrieved significantly fewer per than Importantly, however, subsequent classification rivers detected indicators resulted similar scores that classified random forest majority predictions (72%) from same status categories as Thus, validly communities and, combined learning, provided reliable classifications rivers. while gives macroinvertebrate approach, subsequently calculated indices nevertheless directly comparable consistent.

Language: Английский

Citations

45