Online Direct Infusion Mass Spectrometry of Liquid–Liquid Extraction Phases for Metabolite and Lipid Profiling with the Direct Infusion Probe DOI Creative Commons
Cátia Marques,

Lena Blaase,

Ingela Lanekoff

et al.

Metabolites, Journal Year: 2024, Volume and Issue: 14(11), P. 587 - 587

Published: Oct. 30, 2024

Profiling of metabolites and lipids in biological samples can provide invaluable insights into life-sustaining chemical processes. The ability to detect both the same sample enhance these understandings connect cellular dynamics. However, simultaneous detection is generally hampered by chromatographic systems tailored one molecular type. This void be filled direct infusion mass spectrometry (MS), where all ionizable molecules detected simultaneously. MS, high complexity introduce limitations detectability due matrix effects causing ionization suppression.

Language: Английский

Spatial metabolomics reveal metabolic alternations in the injured mice kidneys induced by triclocarban treatment DOI Creative Commons

Peisi Xie,

Jing Chen, Yongjun Xia

et al.

Journal of Pharmaceutical Analysis, Journal Year: 2024, Volume and Issue: 14(11), P. 101024 - 101024

Published: June 26, 2024

Triclocarban (TCC) is a common antimicrobial agent that has been widely used in medical care. Given the close association between TCC treatment and metabolic disorders, we assessed whether long-term to at human-relevant concentration could induce nephrotoxicity by disrupting levels mouse model. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was applied investigate alterations spatial distributions abundances of TCC, endogenous exogenous metabolites kidney after treatment. The results showed induced changes organ weight, coefficient histopathology kidney. MSI data reveled accumulated all regions kidney, while its five mainly distributed cortex regions. 79 biomolecules associated with pathways leukotriene E4 metabolism, biosythesis degradation glycerophospholipids glycerolipids, ceramide-to-sphingomyelin signaling were significantly altered These distinctive displayed favorable correlation pathological damage. This work offers new insights into related mechanisms TCC-induced nephrotocicity exhibits potential MALDI-MSI-based metabolomics as promising approach for risk assessment agents

Language: Английский

Citations

4
Precision Spatial Control of Tumor‐Stroma Interactions in Cancer Models via 3D Bioprinting for Advanced Research and Therapy DOI
Yan Li, Haijun Cui, Haitao Cui

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract As an emerging technology for modeling cancer tissue in vitro, 3D bioprinting facilitates precise spatial manipulation of cells and biomaterials, allowing the replication complex architectures accurate recapitulation tumor microenvironment (TME) features. This review initially elucidates mechanisms underlying tumorigenesis associated TME complexity, with a particular focus on roles stromal cell populations characteristics extracellular matrix (ECM) progression. Moreover, this work summarizes recent advances bioprinted models, emphasizing their application studying cell‐ECM interactions, stromal‐tumor crosstalk, vasculature formation investigating occurrence metastasis, as well utility high‐throughput drug screening therapeutic development. Finally, discusses advantages challenges creating biomimetic while providing insights into future development trajectories potential translational applications research personalized medicine. By focusing these critical dimensions, aims to highlight transformative role advancing vitro models ultimate goal improving treatment, prevention, patient outcomes.

Language: Английский

Citations

0
Monospecific, ultrasensitive, and quantitative lateral flow immunoassay for dibutyl phthalate via epitopes rational design strategies DOI
Huihui Wu,

Aihong Wu,

Aihua Qu

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 958, P. 177904 - 177904

Published: Dec. 9, 2024

Language: Английский

Citations

1
DHA-enriched phosphatidylserine alleviates bisphenol A-induced liver injury through regulating glycerophospholipid metabolism and the SIRT1-AMPK pathway DOI Creative Commons

Jing Tian,

Yun Lü, Qiaoling Zhao

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(14), P. e34835 - e34835

Published: July 1, 2024

To investigate the alleviating effect and mechanism of docosahexaenoic acid-enriched phosphatidylserine (DHA-PS) on bisphenol A (BPA)-induced liver injury in mice, murine model was established by gavage BPA (5 mg/kg) or co-administration DHA-PS (50 mg/kg 100 for 6 weeks. The results showed that after administration DHA-PS, index, serum levels AST, ALT, TC, TG, NEFA, LDL-C mice were significantly decreased, while HDL-C increased. LPS, IL-6, IL-1β, TNF-α, MDA tissues effectively down-regulated, IL-10, SOD, GSH-Px, CAT up-regulated. H&E Oil Red O staining damage notably repaired lipid deposition reduced administration. Furthermore, metabolomics immunohistochemical studies revealed mainly regulates glycerophospholipid metabolism SIRT1-AMPK pathway to improve metabolic disorders caused BPA. Therefore, could potentially alleviate BPA-induced through suppressing inflammation oxidative stress, modulating disorders.

Language: Английский

Citations

0
Online Direct Infusion Mass Spectrometry of Liquid–Liquid Extraction Phases for Metabolite and Lipid Profiling with the Direct Infusion Probe DOI Creative Commons
Cátia Marques,

Lena Blaase,

Ingela Lanekoff

et al.

Metabolites, Journal Year: 2024, Volume and Issue: 14(11), P. 587 - 587

Published: Oct. 30, 2024

Profiling of metabolites and lipids in biological samples can provide invaluable insights into life-sustaining chemical processes. The ability to detect both the same sample enhance these understandings connect cellular dynamics. However, simultaneous detection is generally hampered by chromatographic systems tailored one molecular type. This void be filled direct infusion mass spectrometry (MS), where all ionizable molecules detected simultaneously. MS, high complexity introduce limitations detectability due matrix effects causing ionization suppression.

Language: Английский

Citations

0