The Chemical Record,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 5, 2024
Abstract
31
P
NMR
spectroscopy
is
a
consolidated
tool
for
the
characterization
of
organophosphorus
compounds
and,
more
recently,
reaction
monitoring.
The
evolution
organic
synthesis,
mainly
due
to
combination
elaborated
building
blocks
with
enabling
technologies,
generated
great
challenges
understand
and
optimize
synthetic
methodologies.
In
this
sense,
experiments
also
became
routine
technique
monitoring,
accessing
products
side
yields,
chiral
recognition,
kinetic
data,
intermediates,
as
well
basic
parameters,
such
acid‐base
hydrogen‐bonding.
This
review
deals
these
aspects
demonstrating
essential
role
spectroscopy.
recent
publications
(the
last
ten
years)
will
be
explored,
discussing
strategies
accomplished
detect
and/or
quantify
distinct
molecules,
approaching
mechanism,
stability,
stereochemistry,
utility
probe.
Biochemical Engineering Journal,
Journal Year:
2024,
Volume and Issue:
205, P. 109260 - 109260
Published: Feb. 14, 2024
Microreactors
have
been
successfully
applied
to
execute
a
broad
range
of
biotransformations
in
flow.
However,
microreactors
typically
designed
with
specific
biotransformation
or
biocatalyst
immobilization
method
mind,
constraining
their
wider
applicability.
Furthermore,
design
is
either
applicable
for
whole-cell
enzyme
biocatalysis,
but
not
both.
We
present
novel
microreactor
which
offers
cartridge-like
insertion
both
immobilised
enzymes
and
cells.
A
T-shaped
lid
opens
closes
the
reaction
chamber
(whilst
leaving
rest
unchanged),
enables
easy
biocatalysts,
thus
allows
user
configure
different
reactor
types.
demonstrated
this
concept
showing
three
types:
hydrogel
containing
entrapped
E.
coli
cells
overexpressing
transketolase
(volumetric
productivity
2.23
±
0.83
mmolL-ERY
Lvoid1
min1),
packed-bed
commercial
beads
Candida
antarctica
lipase
B
317.69
96.74
mmolBB
micropillar
surface-immobilised
ω-transaminase
0.08
0.02
mmolACP
min1).
The
proposed
showed
consistency
robustness
10
consecutive
'open
close'
cycles
withstood
pressure
at
least
4
bar.
Design
analysis
further
included
Computational
Fluid
Dynamics
models
Residence
Time
Distribution
measurements.
presented
standardised
approach
multiple
applications,
underpinning
process
development
paving
way
off-the-shelf
technology
biocatalysis.
Catalysts,
Journal Year:
2024,
Volume and Issue:
14(2), P. 115 - 115
Published: Jan. 31, 2024
The
lipase
from
Prunus
dulcis
almonds
has
been
immobilized
for
the
first
time.
For
this
purpose,
two
different
supports,
an
octadecyl
methacrylate
particulate
support,
and
aminated
agarose
(monoaminoethyl-N-aminoethyl)
have
utilized.
Both
biocatalysts
show
improved
enzyme
stability,
but
great
changes
in
specificity
were
detected.
via
ion
exchange
maintained
its
activity
intact
versus
p-nitrophenyl
butyrate,
while
on
hydrophobic
support
fully
lost
substrate,
which
was
confirmed
to
be
due
substrate
adsorption
support.
However,
biocatalyst
much
more
active
triacetin
(more
than
10-fold),
R-
or
S-
methyl
mandelate
at
pH
7.
At
9,
a
strong
effect
of
using
phosphate
bicarbonate
as
reaction
buffers
Using
bicarbonate,
interfacially
presented
no
R-isomer,
it
very
S-isomer
triacetin.
buffer
during
reaction,
all
compounds
recognized
substrates.
significantly
phosphate;
fact,
inactive
both
isomers.
This
paper
shows
time
interaction
between
effects
immobilization
protocol
used
enantiospecificity
lipases.
ACS Agricultural Science & Technology,
Journal Year:
2025,
Volume and Issue:
5(4), P. 461 - 467
Published: Feb. 24, 2025
Worldwide,
phytopathogenic
fungi,
bacteria,
and
viruses
are
responsible
for
huge
crop
losses
each
year,
threatening
agricultural
progress
food
security
causing
massive
economic
damages.
Pyricularia
oryzae
represents
one
of
the
most
dangerous
fungal
phytopathogens
being
cause
rice
blast,
a
highly
destructive
disease
widely
distributed
across
world.
In
this
critical
context,
good
practices
necessarily
need
to
be
supported
using
novel,
effective,
sustainable
agrochemicals.
It
is
known
that
plants
naturally
counteract
exogenous
infections
by
synthesizing
defense
secondary
metabolites,
as
phytoalexins.
Inspired
N-benzoyltryptamine
N-cinnamoyltryptamine,
two
phytoalexins
found
in
Oryza
sativa,
we
designed
collection
tryptamine-based
derivatives.
The
compounds
were
synthesized
exploiting
an
enzymatic
approach,
Candida
antarctica
Lipase
B
(CaL-B)
biocatalyst
tert-amyl
alcohol
(t-AA)
unconventional
green
solvent.
activity
was
evaluated
against
panel
different
fungi
well
selected
Gram-negative
Gram-positive
bacteria.
obtained
results
pave
way
novel
nature-inspired
products
valuable
alternative
currently
available
pesticides.
Catalysts,
Journal Year:
2024,
Volume and Issue:
14(11), P. 767 - 767
Published: Oct. 30, 2024
Biocatalysis
is
a
green
and
sustainable
technology
based
on
the
use
of
natural
substances
to
catalyze
chemical
reactions.
Humans
have
been
unconsciously
using
biocatalysis
for
thousands
years
produce
food
alcoholic
beverages,
but
it
only
since
19th
century
that
we
begun
understand
its
fundamentals
enormous
potential.
In
fact,
advances
in
our
knowledge
enzymes
metabolic
pathways
and,
recent
decades,
introduction
tools
such
as
bioinformatics,
DNA
sequencing
protein
engineering
made
key
strategy
fine
chemistry
production
active
pharmaceutical
ingredients.
addition,
discovery
new
microorganisms
adapted
adverse
conditions
has
also
crucial
advancing
this
avenue.
The
present
review
focuses
unconventional
yeasts
their
most
interesting
reactions
where
applied.
It
highlights
advantages
these
industrial
processes
due
particular
phenotypes,
ability
withstand
high
temperatures
pressures,
well
acidic
or
alkaline
environments,
substrate
loads,
presence
organic
solvents,
etc.
All
results
wider
range
possible
substrates
higher
efficiency.
Examples
important
which
described
are
included,
considering
both
catalysis
by
wild-type
whole
cells
isolated
genetically
modified
variants.
information
will
help
current
relevance
biocatalysis.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(18), P. 10057 - 10057
Published: Sept. 19, 2024
Tyrosol
and
hydroxytyrosol
are
powerful
phenolic
antioxidants
occurring
in
olive
oil
by-products
from
processing.
Due
to
their
high
polarity,
esterification
or
other
lipophilization
is
necessary
make
them
compatible
with
lipid
matrices.
Hydroxytyrosol
methyl
carbonate
a
more
effective
antioxidant
than
dibutylhydroxytoluene
α-tocopherol
together
tyrosol
exerts
interesting
pharmacological
properties.
The
purpose
of
this
work
was
the
enzymatic
preparation
alkyl
carbonates
hydroxytyrosol.
A
set
17
hydrolases
tested
catalysis
methoxycarbonylation
neat
dimethyl
find
an
economically
feasible
alternative
recently
reported
synthesis
catalyzed
by
Novozym
435.
435
was,
however,
found
be
best
performing
catalyst,
while
735,
pig
pancreatic
lipase,
lipase
F-AK
Lipex
100T
exhibited
limited
reactivity.
No
enzyme
accepted
1,2-propylene
as
acylation
donor.
Under
optimized
reaction
conditions,
used
batch
quantitative
yields.
can
also
method
for
selective
protection
syntheses
phenylethanoid
glycosides
enzymes
comprising
levels
acetyl
esterase
side
activity.
