Design and engineering of neuroglobin to catalyze the synthesis of indigo and derivatives for textile dyeing

Molecular Systems Design & Engineering, Journal Year: 2021, Volume and Issue: 7(3), P. 239 - 247

Published: Nov. 30, 2021

Human neuroglobin (Ngb) was rationally engineered into an enzyme by triple mutations, A15C/H64D/F49Y Ngb, which efficiently catalyzes the synthesis of indigo and derivatives with different colors for textile dyeing.

Language: Английский

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Comparison of Laccases and Hemeproteins Systems in Bioremediation of Organic Pollutants

Current Protein and Peptide Science, Journal Year: 2022, Volume and Issue: 23(6), P. 402 - 423

Published: June 1, 2022

Laccases and peroxidases have attracted great interest for industrial environmental applications. These enzymes a broad substrate range robust oxidizing ability. Moreover, using mediators or co-oxidants makes it possible to increase their catalytic activity extend scope more resistant chemical structures.Fungal laccases ligninolytic peroxidases, mainly lignin manganese are the privileged oxidoreductases bioremediation processes. Nonetheless, an increasing diversity of peroxidase-type has been proposed technologies.This article aims provide overview these compare applicability in degradation organic pollutants.Fundamental properties proteins covered applications towards polycyclic aromatic hydrocarbons (PAHs) pesticides specially focused.Laccases multicopper oxidases initially studied pulp paper industry but able oxidize variety environmentally concerning compounds. Relying on O2, do not require peroxides nor auxiliary agents, like Mn2+, although suitable redox needed attack recalcitrant pollutants (e.g., PAHs). True pseudo-peroxidases use stronger oxidant (H2O2) chemistry at heme site generates high potential species that allow oxidation dyes some pesticides.Lately, research efforts directed enzyme discovery, testing with micropollutants, improving biocatalysts' stability by immobilization protein engineering. Further understanding effects natural media components solvents might lead competitive enzymatic treatments highly toxic media.

Language: Английский

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Spiro-Oxindole Skeleton Compounds Are Efficient Inhibitors for Indoleamine 2,3-Dioxygenase 1: An Attractive Target for Tumor Immunotherapy

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(9), P. 4668 - 4668

Published: April 23, 2022

Indoleamine 2,3-dioxygenase 1 (IDO1) is an attractive heme enzyme for its significant function in cancer immunotherapy. Potent IDO1 inhibitors have been discovered decades, whereas no clinical drugs are used treatment up to now. With the goal of developing medically valuable IDO inhibitors, we performed a systematic study SAR405838 analogs with spiro-oxindole skeleton this study. Based on expression and purification human IDO1, inhibitory activity compounds was evaluated by IC50 Ki values. The results demonstrated that inhibitor 3 exhibited highest at 7.9 μM among all which ~six-fold positive control (4−PI). Moreover, found most effective inhibition MCF-7 cells without toxic effects. Molecular docking analysis revealed hydrophobic interaction stabilized binding active site made explanation uncompetitive mode inhibitors. Therefore, provides insights into screen more potent

Language: Английский

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Effects of naturally occurring S47F/A mutations on the structure and function of human cytochrome c

Journal of Inorganic Biochemistry, Journal Year: 2023, Volume and Issue: 246, P. 112296 - 112296

Published: June 17, 2023

Language: Английский

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Improving the cell-membrane-penetrating activity of globins by introducing positive charges on protein surface: A case study of sperm whale myoglobin

Biochemical and Biophysical Research Communications, Journal Year: 2022, Volume and Issue: 598, P. 26 - 31

Published: Feb. 6, 2022

Language: Английский

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