Hybrid Se/melanin-like nanoparticles as ROS quenchers and inhibitors of amyloid aggregation DOI Creative Commons
Giulio Pota, Concetta Di Natale,

Antonia Puzone

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 312, P. 144175 - 144175

Published: May 14, 2025

Hybrid selenium/melanin-like nanoparticles offer different therapeutic strategies against amyloid aggregation. In this study, novel Se-based nanostructures are synthesized, characterized, and preliminarily employed as modulators of detail, two types Se NPs tested: one containing only Se(0), named NPs, the second hybrid Se/melanin structures, indicated SeMel NPs. Advanced biophysical spectroscopic analyses elucidate structures mechanistic underpinnings inhibition aggregation protein fragments models, Aβ21-40 NPM1264-277. Both investigated for their antioxidant superoxide dismutase (SOD)-like activity, well colloidal stability through dynamic light scattering (DLS) ζ-potential measurements. ThT SEM experiments demonstrate ability to suppress aggregation, while far-UV circular dichroism (CD) spectroscopy indicates secondary structure alterations upon nanoparticle interaction, revealing a shift from β-sheet-rich conformations towards α-helical intermediates. Finally, cytocompatibility with SH-SY5Y cells an effective mitigation cytotoxic effects models. These findings position Se-melanin promising agents targeted therapeutics.

Language: Английский

Hybrid Se/melanin-like nanoparticles as ROS quenchers and inhibitors of amyloid aggregation DOI Creative Commons
Giulio Pota, Concetta Di Natale,

Antonia Puzone

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 312, P. 144175 - 144175

Published: May 14, 2025

Hybrid selenium/melanin-like nanoparticles offer different therapeutic strategies against amyloid aggregation. In this study, novel Se-based nanostructures are synthesized, characterized, and preliminarily employed as modulators of detail, two types Se NPs tested: one containing only Se(0), named NPs, the second hybrid Se/melanin structures, indicated SeMel NPs. Advanced biophysical spectroscopic analyses elucidate structures mechanistic underpinnings inhibition aggregation protein fragments models, Aβ21-40 NPM1264-277. Both investigated for their antioxidant superoxide dismutase (SOD)-like activity, well colloidal stability through dynamic light scattering (DLS) ζ-potential measurements. ThT SEM experiments demonstrate ability to suppress aggregation, while far-UV circular dichroism (CD) spectroscopy indicates secondary structure alterations upon nanoparticle interaction, revealing a shift from β-sheet-rich conformations towards α-helical intermediates. Finally, cytocompatibility with SH-SY5Y cells an effective mitigation cytotoxic effects models. These findings position Se-melanin promising agents targeted therapeutics.

Language: Английский

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