Antimicrobial activity of stingless bee honey (Tribe: Meliponini) on clinical and foodborne pathogens: A systematic review and meta‐analysis

Food Frontiers, Journal Year: 2024, Volume and Issue: 5(3), P. 964 - 993

Published: March 18, 2024

Abstract Honey produced by stingless bees (Tribe: Meliponini ) is well‐known for their medicinal and antimicrobial properties from diverse content of bioactive compounds. However, it has not been possible to fully characterize its active principles. The present systematic review based on 117 full‐text articles. Our highlights (1) the need apply complementary methodologies consolidate protocols quantify activity (2) agents in honeys Heterotrigona itama , Tetragonisca angustula Melipona beecheii and; (3) search compounds against multidrug‐resistant and/or biofilm‐forming pathogens. Moreover, we performed a meta‐analysis 29 articles with quantitative data using diffusion assays minimum inhibitory concentration (MIC). Staphylococcus aureus Escherichia coli were most evaluated pathogens, evidencing 221 149 honey samples 36 24 bee species, respectively. highest pooled mean S. showed difference between methicillin‐resistant (MRSA) non‐MRSA strains {14.43 [95% confidence interval (CI): 12.16–16.71] mm vs. 11.55 (95% CI: 10.22–12.87) mm}, whereas E. means 9.09 7.93–10.25) mm. Regarding MIC expressed as volume/volume percentage (%, v/v), estimated concentrations 7.89 3.94–11.83) mL/100 mL MRSA 5.60 2.66–8.55) considering all strains. In contrast, MICs weight/volume w/v) 15.00 [(95% 12.84–17.16) g/100 16.17 5.78–26.55) . remains an unexplored source molecules.

Language: Английский

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Characterization of Larvicidal Diterpene Resin Acids in Melipona quadrifasciata Geopropolis via LC‐ESI‐MS/MS, GC–MS and Computational Analysis

Rapid Communications in Mass Spectrometry, Journal Year: 2025, Volume and Issue: 39(12)

Published: March 21, 2025

Dengue, an arboviral disease transmitted by the Aedes aegypti mosquito, is a major global public health problem challenge. Insecticides based on natural products can provide good alternative to synthetic agents, as they are safer for both environment and human health. This study evaluated activity of geopropolis from stingless bees Apis mellifera Ae. aegypti, using mass spectrometry approaches identify compounds with larvicidal potential against aegypti. The propolis A. mellifera, well Melipona quadrisfasciata (a mixture soil/clay propolis), was larvae (Rockefeller strain). ESI-MS/MS analyses were performed quadrupole/time-of-flight spectrometer all samples, quadrifasciata also analyzed ion trap instrument. ESI-qTOF-MS/MS data processed in spectral similarity network GNPS. Molecular annotation silico tool called NAP. Gas-phase fragmentation mechanisms proposed conjunction computational chemistry studies. Silylated samples GC-MS. Geopropolis bee M. caused 90% 100% mortality after 24 48 h exposure, respectively, exhibiting highest activity. Mass spectrometry-based molecular approach supported suggestion discriminant between active inactive samples. combination NAP predictions gas-phase reactions EI-MS facilitated compounds, including diterpene resin acids, such dehydroabietic acid its derivatives, abietic acid, pimaranes. HPLC-MS/MS GC-MS suggests that acids contribute effect enhancing our understanding potentially bioactive arbovirus vector.

Language: Английский

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Selected honey as a multifaceted antimicrobial agent: review of compounds, mechanisms, and research challenges

Future Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 22

Published: April 28, 2025

Honey, derived from floral nectar, has been valued for its nutritional and therapeutic properties, with recent studies emphasizing broad-spectrum antimicrobial potential, especially against resistance (AMR). Honey's activity stems unique composition, including high sugar content, low pH, bioactive compounds like hydrogen peroxide, methylglyoxal (MGO), phenolic compounds. Distinct honey types, such as Manuka, Sidr, Tualang, demonstrate varying effects based on their botanical geographical origins. Manuka honey, rich in MGO, is notably effective multidrug-resistant pathogens, while Sidr heather honeys excel biofilm inhibition antioxidative properties. Bioactive components, phenolics, flavonoids, enzymes, peptides, disrupt microbial membranes, inhibit metabolic pathways, induce oxidative stress. Advanced analytical techniques HPLC GC-MS have identified these compounds, though gaps remain understanding secondary metabolites synergistic actions. This review highlights honey's potential a sustainable resource, the need standardization, clinical validation, interdisciplinary research. Honey represents promising solution to AMR offers opportunities integration into modern medicine healthcare strategies.

Language: Английский

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A narrative review on the physicochemical profiles, bioactive compounds, and therapeutic potentials of stingless bee honey

Discover Food, Journal Year: 2025, Volume and Issue: 5(1)

Published: May 22, 2025

Language: Английский

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Evaluating the Effects of Flavonoids on Insects: Implications for Managing Pests Without Harming Beneficials

Insects, Journal Year: 2024, Volume and Issue: 15(12), P. 956 - 956

Published: Dec. 1, 2024

Flavonoids have multiple functions, including host-plant defense against attacks from herbivorous insects. This manuscript reviewed and analyzed the scientific literature to test hypothesis that flavonoids can be utilized manage pests without causing significant harm beneficials. The methodology involved using recognized databases, e.g., Web of Science, Scopus, CAB Abstracts, via USDA-ARS, National Agricultural Library, DigiTop retrieval system. Data were compiled in tables subjected statistical analysis, when appropriate. generally harmful true bugs flies but harmless honey bees. Flavonoid glycosides showed a tendency (Heteroptera) (Diptera). sawflies. flavonoid produced mixture outcomes beetles, depending on species. butterflies. In conclusion, specific could function as feeding stimulants or deterrents, oviposition chemical protectants pesticides, mating attractants, less-toxic insecticides, other functions. some insect beneficials (e.g., bees). Flavonoid-based insecticides serve environmentally benign alternatives broad-spectrum pests, field testing is necessary.

Language: Английский

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Brazilian stingless bee honey: A pioneer study on the in vitro bioaccessibility of phenolic compounds

Food Chemistry, Journal Year: 2024, Volume and Issue: 460, P. 140332 - 140332

Published: July 3, 2024

Language: Английский

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Insularity and its impact on stingless bee honey properties: A case study in the Zanzibar Archipelago (Tanzania)

JSFA reports, Journal Year: 2023, Volume and Issue: 4(2), P. 64 - 71

Published: Nov. 27, 2023

Abstract Background Islands are known to host unique flowering plant assemblages visited by insular insects, including social bees, a phenomenon thought potentially impact the honey composition and associated medicinal applications. In this study, we characterized several physical–chemical parameters of produced stingless bee Meliponula (Axestotrigona) ferruginea with samples collected both from continental (Kenya) (Tanzania) ecosystems. Results Our results show that bees in islands produce whose significantly differs those continent, higher concentration bioactive phytochemicals. Conclusion illustrate how insularity might drive different compositional profiles honeys provide evidence certain groups compounds found Afrotropical region have potential therapeutic benefits applications largely remain be explored.

Language: Английский

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ВЕТЕРИНАРНО-САНІТАРНА ОЦІНКА МЕДУ ТА ІНШИХ ПРОДУКТІВ БДЖІЛЬНИЦТВА ЗА ПОКАЗНИКАМИ ЯКОСТІ І БЕЗПЕЧНОСТІ

Bulletin of Sumy National Agrarian University The series Livestock, Journal Year: 2024, Volume and Issue: 1, P. 48 - 58

Published: March 12, 2024

Метою здійснення огляду було надати ветеринарно-санітарну оцінку меду та іншим продуктам бджільництва за показниками якості і безпечності у світлі сучасних досліджень для забезпечення безпеки споживача. Великою проблемою сьогодення є зниження захисних функцій організму населення України, збільшення онкологічних, серцево-судинних інших захворювань, що пов’язано зі зниженням активності антиоксидантної системи рахунок впливу неповноцінного харчування, стресів (війна), забруднення навколишнього середовища радіоактивними іншими шкідливими речовинами. Антиоксиданти природного або штучного походження здатні зв’язувати зайві вільні радикали перешкоджають прискореному окисленню ліпідів утворенню небажаних продуктів окислення, а отже зміцнюють захисні функції організму. Найкращими джерелами природних антиоксидантів продукти бджільництва. Мед містить значну кількість ферментів, фенолів, органічних кислот флавоноїдів. Особливо багато в темних сортах меду. Науково підтверджені антибактеріальні властивості бджільництва: меду, прополісу, бджолиної отрути, перги, маточного молочка, бджолиного обніжжя. Проте мають високі адсорбційні властивості, тому можуть бути потенційно небезпечними здоров’я людини внаслідок накопичення шкідливих речовин, знаходяться ґрунті, воді повітрі застосованих лікування бджіл антибактеріальних препаратів. Велику небезпеку становить застосування рослинництві препаратів системної дії, накопичуватися пилку нектарі. Оскільки ціни на мед 5-10 разів вищі, ніж цукор, то він часто піддається фальсифікації. Тому його якість безпечність відносяться до національних пріоритетів будь-якої держави. Для виробництва безпечного бджільництва, їх конкурентоспроможності зовнішньому ринку довіру споживачів внутрішньому період техногенного середовища, необхідно здійснювати посилений ветеринарно-санітарний контроль всіх рівнях – від виробництва, зберігання, постачальника-заготівельника роздрібного споживача, проводити моніторингові дослідження, важливою складовою продовольчої безпеки. Актуальним систематичний перегляд вітчизняної нормативної документації яка потребує оптимізації.

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The Effect of Stingless Bee Honey Mouthrinse on Dental Plaque Accumulation: A Randomised Clinical Trial

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 29, 2024

Background: Mouthrinses are recommended as an adjunct to mechanical methods in oral hygiene procedures for the prevention and management of periodontal diseases. Stingless bee honey (SBH) is a natural food with many health benefits. It has been reported be beneficial cavity such antibacterial, anti-inflammatory, healing properties making it potential therapeutic agent therapy. This study aims investigate ability stingless mouthrinse reduce plaque accumulation assess patients’ acceptance towards its use. Methods: pilot randomised clinical trial performed on volunteers (N=30) healthy gingiva and/or mild gingivitis randomly prescribed SBH, chlorhexidine (CHX) or normal saline (NS) without practice three days. Plaque patient perception were recorded post-intervention using score visual analogue scale (VAS) respectively. Results: Post-intervention after days, there was no significant change SBH NS groups (p>0.05) compared baseline. Significant reduction found CHX group (p<0.05) A significantly better taste lower burning sensation Conclusions: SBH effectively prevented increase absence hygiene, less sensation. However, prevent accumulation. Trial registration: study was retrospectively registered 24/1/2024 at ClinicalTrials. gov (No. NCT06223243).

Language: Английский

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LC-HRMS based metabolite profiling of black wheat rawa idli incorporated panner whey powder reveals key bioactive compounds

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 6, 2024

The whey waste from dairy processing is rich in valuable nutrients like amino acids, proteins etc. which offer various health benefits. Finding ways to utilize this and incorporate it into food products an intriguing approach. Advanced analytical techniques liquid chromatography-mass spectrometry have become important tools for comprehensively analyzing the composition of foods. aim study optimized black wheat-based rawa idli that had been formulated with different levels paneer powder (PWP). This analysis was able identify 40 distinct functional compounds within matrix. Some most notable found included ferulic acid, rutin, isorhamnetin all are known diverse promoting properties. results highlight bioactive potential incorporating dairy-derived ingredient (PWP) wheat idli. Adding PWP appears enhance overall qualities food, making a promising as healthy, value-added product.

Language: Английский

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