The Fall Armyworm Spodoptera frugiperda Found on Rice Oryza sativa L. in China: Their Host Strain, Oviposition Preference and Survival Rate on Rice and Maize

Agronomy, Journal Year: 2024, Volume and Issue: 14(10), P. 2344 - 2344

Published: Oct. 11, 2024

The fall armyworm (FAW), Spodoptera frugiperda, is a serious pest that threatens range of important crops worldwide. It originated in America and rapidly dispersed throughout Africa Asia 2018. There are two subtypes, corn-strain (C-strain) rice-strain (R-strain), have different host plant preferences, the individuals damaging maize China were identified as C-strain. In present study, we found FAW rice plants field Guangdong Province, China. larvae male adults collected, majority FAWs characterized CO I R-strain Tpi C-strain, which similar to preferred laying eggs on more than plants. Compared those fed leaves, unable survive when 4-week-old plants, whereas they could complete their life cycle 2-week-old for total survival rate was 8%. pre-adult- pupal-stage durations prolonged, fecundity adult females decreased. Thus, paddy fields showed better fitness laboratory. Owing low unlikely damage large areas, but populations should be monitored.

Language: Английский

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Response patterns of lncRNAs of theSpodoptera frugiperda(Lepidoptera: Noctuidae) larvae under 23 pesticide treatments

Journal of Insect Science, Journal Year: 2023, Volume and Issue: 23(4)

Published: July 1, 2023

The response of Spodoptera frugiperda genes toward insecticides is crucial for guiding insecticide use. regulation the S. via long noncoding RNAs (lncRNAs) under treatment should be investigated. In this study, 452 differentially expressed lncRNAs were identified by analyzing RNA-sequencing data 23 pesticide treatments. We found 59 and 43 that could regulate detoxification-related cytochrome P450 UDP-glucuronosyltransferase genes, respectively. Furthermore, target enriched in Pfam, including chitin bind 4 gene ontology terms such as structural constituent cuticle, revealing their potential mechanism action on growth inhibition larvae. Insecticide-specific expression highlights properties commonalities different insecticide-induced lncRNA regulatory mechanisms. To conclude, results study provide new insights perspectives use mRNAs.

Language: Английский

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Uninheritable but widespread bacterial symbiont mediates insecticide detoxification of an agricultural invasive pestSpodoptera frugiperda

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 27, 2023

Abstract Identifying insecticide resistance-related insect bacterial symbiont is a vital compass for promoting symbiont-based resistance management and insecticide-free pest control. Known insecticide-resistance-related symbionts lack universality genetic manipulation, as either not widespread or unculturable. Here, we discovered Enterococcus casseliflavus of significant invasive pest, Spodoptera frugiperda . This enhances host to chlorantraniliprole by amide bond breaking dehalogenation-related degradation, suggesting its great potential Lepidoptera management. Complying with the increase in exposure risk chlorantraniliprole, E. insects’ rather than mammals environmental strains were notably enriched putative degradation genes. We also found that can transmit horizontally high efficiency (100%) through cross-diet cannibalism vertical transmission from mother offspring. Moreover, infection field populations only implies an underlying symbiont-host co-evolution process driven pressure might be underway, but provides novel therapeutic target agricultural pests based on symbiont-targeted control (STIC).

Language: Английский

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Fighting caterpillar pests and managing agricultural insecticide resistance with Lepidoptera-associated <i>Enterococcus casseliflavus</i>

The Innovation Life, Journal Year: 2023, Volume and Issue: 1(3), P. 100042 - 100042

Published: Jan. 1, 2023

<p>Probiotics are live microorganisms that intended to benefit the health of metazoan hosts. Mutualism in insects (both pests and beneficial insects) provides a vital contribution host fitness. With development demand for healthy modern agriculture, bacterial symbionts considered be an important toolbox pest control insect protection. <i>Enterococcus casseliflavus</i> is widespread symbiont most Lepidoptera pests. Recent studies indicated <i>E. phenotypes host, including supplying diet lacking amino acids enhancing insecticide resistance. We propose should have enormous biotechnological potential awaiting further exploitation or protection through symbiont-targeted resistance management, because this natural caterpillar hosts, easily isolatable cultured, rapidly spreadable within population. outline rationales why suitable application management envision possible plans therapeutics via four approaches: genome modification, phage engineering, predatory biocontrol, synthetic microbiome. This perspective opens up grand blueprint future suppression economic promotion.</p>

Language: Английский

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Identification of Spodoptera frugiperda (Lepidoptera: Noctuidae) and its two host strains in China by PCR-RFLP

Journal of Economic Entomology, Journal Year: 2023, Volume and Issue: 116(3), P. 983 - 992

Published: April 29, 2023

Abstract The fall armyworm (FAW) Spodoptera frugiperda was first found in China 2018. In other countries, FAW has evolved corn and rice strain biotypes. It is not possible to identify these strains based on morphology. addition, very similar appearance several common pests. These situations bring great challenges the population management of FAW. this study, we developed a rapid identification method PCR-RFLP distinguish two from lepidopteran A 697 bp mitochondrial cytochrome c oxidase I (COI) cloned sequenced FAW, litura, exigua, Mythimna separata. COI fragments species revealed unique digestion patterns created by three enzymes (Tail, AlWN I, BstY II). Thus, four can be distinguished each other. enzyme Ban recognized SNP site 638 triosephosphate isomerase (Tpi) fragment Tpi cut into bands. However, could digested. Using method, all 28 samples collected different host plants locations were identified as strain. This suggests that yet invaded China. allows discrimination Lepidopteran pests distinguishes strains.

Language: Английский

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