Journal of Industrial Microbiology & Biotechnology,
Journal Year:
2023,
Volume and Issue:
50(1)
Published: Jan. 1, 2023
Rhodotorula
toruloides
is
being
developed
for
the
use
in
industrial
biotechnology
processes
because
of
its
favorable
physiology.
This
includes
ability
to
produce
and
store
large
amounts
lipids
form
intracellular
lipid
bodies.
Nineteen
strains
were
characterized
mating
type,
ploidy,
robustness
growth,
accumulation
on
inhibitory
switchgrass
hydrolysate
(SGH).
Mating
type
was
determined
using
a
novel
polymerase
chain
reaction
(PCR)-based
assay,
which
validated
classical
microscopic
test.
Three
heterozygous
(A1/A2).
Ploidy
analysis
revealed
complex
pattern.
Two
triploid,
eight
haploid,
either
diploid
or
aneuploid.
A1/A2
compared
their
parents
growth
75%v/v
concentrated
SGH.
The
much
more
robust
than
parental
strains,
did
not
grow
had
extended
lag
times.
entire
set
evaluated
60%v/v
SGH
batch
cultures
kinetics
biomass
production.
Lipid
titers
2.33-9.40
g/L
with
median
6.12
g/L,
excluding
two
that
grow.
yields
0.032-0.131
(g/g)
contents
13.5-53.7%
(g/g).
Four
significantly
higher
contents.
One
these
among
highest
yield
this
study
(0.131
±
0.007
g/g),
has
been
previously
described
literature.The
yeast
used
oil
sugars
extracted
from
bioenergy
grass.
Foods,
Journal Year:
2025,
Volume and Issue:
14(4), P. 673 - 673
Published: Feb. 17, 2025
Microbial
foods
include
microbial
biomass,
naturally
fermented
foods,
and
heterologously
synthesized
food
ingredients
derived
from
fermentation.
Terpenoids,
using
isoprene
as
the
basic
structure,
possess
various
skeletons
functional
groups.
They
exhibit
diverse
physicochemical
properties
physiological
activities,
such
unique
flavor,
anti-bacterial,
anti-oxidant,
anti-cancer,
hypolipemic,
making
them
extensively
used
in
industry,
fragrance,
preservatives,
dietary
supplements,
medicinal
health
food.
Compared
to
traditional
strategies
like
direct
extraction
natural
species
chemical
synthesis,
cell
factories
for
edible
terpenoids
have
higher
titers
yields.
can
utilize
low-cost
raw
materials
are
easily
scaling-up,
representing
a
novel
green
sustainable
production
mode.
In
this
review,
we
briefly
introduce
synthetic
pathway
of
applications
producing
terpenoids.
Secondly,
highlight
several
typical
non-typical
chassis
terpenoid-producing
factories.
addition,
reviewed
recent
advances
representative
terpenoid
with
gram-scale
titer
preservation,
nutritional
enhancers,
foods.
Finally,
predict
future
directions
their
commercialization
process.
Biotechnology for Biofuels and Bioproducts,
Journal Year:
2024,
Volume and Issue:
17(1)
Published: May 13, 2024
Abstract
Background
Ergothioneine
(EGT)
is
a
distinctive
sulfur-containing
histidine
derivative,
which
has
been
recognized
as
high-value
antioxidant
and
cytoprotectant,
wide
range
of
applications
in
food,
medical,
cosmetic
fields.
Currently,
microbial
fermentation
promising
method
to
produce
EGT
its
advantages
green
environmental
protection,
mild
condition,
low
production
cost.
However,
due
the
low-efficiency
biosynthetic
process
numerous
cell
factories,
it
still
challenge
realize
industrial
biopreparation
EGT.
The
non-conventional
yeast
Rhodotorula
toruloides
considered
potential
candidate
for
production,
thanks
safety
animals
natural
ability
synthesize
Nevertheless,
synthesis
efficiency
deserves
further
improvement.
Results
In
this
study,
out
five
target
wild-type
R.
strains,
2.1389
(RT1389)
was
found
accumulate
highest
could
reach
79.0
mg/L
at
shake
flask
level
on
7th
day.
To
achieve
iterative
genome
editing
strain
RT1389,
CRISPR-assisted
Cre
recombination
(CACR)
established.
Based
it,
an
EGT-overproducing
RT1389-2
constructed
by
integrating
additional
copy
core
genes
RtEGT1
RtEGT2
into
genome,
titer
1.5-fold
increase
over
RT1389.
As
supply
S-adenosylmethionine
identified
key
factor
determining
subsequently,
series
gene
modifications
including
rebalancing
were
integrated
RT1389-2,
resulting
mutants
rapidly
screened
according
their
titers
with
high-throughput
screening
based
ergothionase.
result,
engineered
named
RT1389-3
selected
267.4
after
168
h
50
mL
modified
medium.
Conclusions
This
study
characterized
capacity
these
demonstrated
that
CACR
allowed
rapid
engineering
improved
production.
Furthermore,
provided
remarkable
performance,
had
application
prospects.
Biotechnology for Biofuels and Bioproducts,
Journal Year:
2024,
Volume and Issue:
17(1)
Published: May 28, 2024
Abstract
Background
The
red
oleaginous
yeast
Rhodotorula
toruloides
is
a
promising
cell
factory
to
produce
microbial
oils
and
carotenoids
from
lignocellulosic
hydrolysates
(LCH).
A
multi-stress
tolerant
strain
towards
four
major
inhibitory
compounds
present
in
LCH
methanol,
was
derived
our
laboratory
IST536
(PYCC
5615)
through
adaptive
evolution
(ALE)
under
methanol
high
glycerol
selective
pressure.
Results
Comparative
genomic
analysis
suggested
the
reduction
of
original
ploidy
triploid
diploid,
occurrence
21,489
mutations,
242
genes
displaying
copy
number
variants
evolved
strain.
Transcriptomic
identified
634
with
altered
transcript
levels
(465
up,
178
down)
Genes
associated
surface
biogenesis,
integrity,
remodelling
involved
stress-responsive
pathways
exhibit
most
substantial
alterations
at
genome
transcriptome
levels.
Guided
by
stress
responses,
tolerance
phenotype
extended
osmotic,
salt,
ethanol,
oxidative,
genotoxic,
medium-chain
fatty
acid-induced
stresses.
Conclusions
comprehensive
this
provided
opportunity
get
mechanistic
insights
into
acquisition
list
genes,
pathways,
regulatory
networks,
as
targets
for
synthetic
biology
approaches
applied
factories,
toward
more
robust
superior
industrial
strains.
This
study
lays
foundations
understanding
mechanisms
underlying
multiple
stresses
R.
,
underscoring
potential
ALE
enhancing
robustness
