Effect of the Combination of Clostridium butyricum and Mycelium of Phellinus igniarius on Intestinal Microbiota and Serum Metabolites in DSS-Induced Colitis

Nutrients, Journal Year: 2024, Volume and Issue: 16(1), P. 153 - 153

Published: Jan. 2, 2024

Clostridium butyricum (CB) and Phellinus igniarius (PI) have anti-inflammatory, immune regulation, anti-tumor, other functions. This study aimed to explore the therapeutic effect of CB mycelium PI (MPI) alone in combination on colitis mice induced by dextran sodium sulfate (DSS). Mice were randomly assigned five groups: (1) control (CTRL), (2) DSS, (3) CB, (4) MPI, (5) + MPI (CON). The weight was recorded daily during experiment, length colon measured last day experiment. colons collected for hematoxylin eosin staining, contents intestinal flora analysis, serum metabolite analysis. results showed that compared with DSS group, CON treatments inhibited loss shortening caused significantly increased concentrations interleukin (IL)-4, IL-10, superoxide dismutase, decreased IL-6, tumor necrosis factor-α, myeloperoxidase. Gene sequence analysis 16S rRNA changed composition structure microorganisms. Metabolome metabolites DSS-treated mice, including dodecenoylcarnitine, L-urobilinogen, citric acid. In conclusion, alleviated DSS-induced regulating metabolites, group having best effect.

Language: Английский

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Formation and Characterization of Mycelium–Potato Protein Hybrid Materials for Application in Meat Analogs or Substitutes

Foods, Journal Year: 2024, Volume and Issue: 13(24), P. 4109 - 4109

Published: Dec. 19, 2024

There is increasing interest in the development of meat analogs due to growing concerns about environmental, ethical, and health impacts livestock production consumption. Among non-meat protein sources, mycoproteins derived from fungal fermentation are emerging as promising alternatives because their natural fibrous structure, high nutritional content, low environmental impact. However, poor gelling properties limit application creating analogs. This study investigated potential by combining mycoprotein (MCP), a mycelium-based protein, with potato (PP), plant-based create hybrid products meat-like structures textures. The PP-MCP composites were evaluated for physicochemical, rheological, textural, microstructural using electrophoresis, differential scanning calorimetry, dynamic shear rheology, texture profile analysis, confocal fluorescence microscopy, electron microscopy analyses. gels stronger had more than simple PP gels, which was mainly attributed presence hyphae fibers mycelia. Dynamic rheology showed that hybrids formed irreversible heat-set setting temperature around 70 °C during heating, unfolding aggregation proteins. Confocal analyses contained network mycelia embedded within matrix. hardness could be increased raising content. These findings suggest may useful

Language: Английский

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Isolation and Evaluation of Erinacine A Contents in Mycelia of Hericium erinaceus Strains

Foods, Journal Year: 2024, Volume and Issue: 13(11), P. 1649 - 1649

Published: May 24, 2024

Hericium erinaceus has long been favored for its remarkable nutritional and health-promoting benefits, erinacine A is the key component responsible neuroprotective properties of H. erinaceus. Establishing an efficient method separating from screening A-enriched strains crucial to maximizing benefits. Herein, we first reported that high-speed counter current chromatography (HSCCC) effective high-purity A. Using a two-phase solvent system composed n-hexane/ethyl acetate/methanol/water (4.5:5:4.5:5, v/v/v/v), with purity over 95% was separated. Then, evaluated content yield in liquid-fermented mycelia germplasms. Both varied greatly among surveyed strains. The significant effect strain on revealed by analysis variance. highest were observed wild HeG, reaching 42.16 mg/g 358.78 mg/L, which superior outcomes achieved using submerged cultivation. isolation established screened this study can be beneficial scaling up extraction nutraceutical development industrial levels.

Language: Английский

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Characterization, optimization and scaling up of submerged Inonotus hispidus mycelial fermentation for enhanced biomass and polysaccharide production

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

This study was to establish an efficient strategy based on inoculum-morphology control for the submerged mycelial fermentation of edible and medicinal fungus, Inonotus hispidus. Two major morphological forms inoculum were compared, dispersed fragments versus aggregated clumps. The one more favorable fermentation, starting with a shorter lag period attaining higher biomass yield uniform mycelium pellets in shake-flasks. taken from shake-flask culture day 6 fragmented at 26,000 rpm homogenizer, shear time 3 min provided optimal inoculum. conditions further verified 5-L stirred-tank fermenters then scaled-up 100 L stirred-tank. With optimized process plus fed-batch operation, much productivities, including 22.23 g/L biomass, 3.31g/L EPS, 5.21g/L IPS, achieved 100-L fermenter than flask culture. Composition analysis showed that I. hispidus produced by rich protein, dietary fiber, polysaccharides which may be benefitical health. Overall, results have shown characteristics age, morphology state aggregation significant impact productivity fungus.

Language: Английский

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Characterization, Optimization, and Scaling-up of Submerged Inonotus hispidus Mycelial Fermentation for Enhanced Biomass and Polysaccharide Production

Applied Biochemistry and Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 25, 2024

Language: Английский

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