From trash to cash: current strategies for bio-upcycling of recaptured monomeric building blocks from poly(ethylene terephthalate) (PET) waste

Green Chemistry, Journal Year: 2024, Volume and Issue: 26(10), P. 5708 - 5743

Published: Jan. 1, 2024

Bio-upcycling of monomers recovered from poly(ethylene terephthalate) (PET) waste is a novel alternative to turn recycling process more economically attractive by obtaining several products with higher added value.

Language: Английский

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Characterization and functional reconstruction of a highly productive germacrene A synthase from Liriodendron chinense

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

Summary Plants produce a large array of natural products which play important roles in flavours, fragrances and medicines. However, some high‐value plant intermediate metabolites cannot be directly extracted from plants. The tulip tree ( Liriodendron chinense ) the Magnoliaceae family is rich sesquiterpenes. Upon characterizing functions 11 terpene synthases, we discovered that LcTPS3 could high yields (+)‐germacrene A, was shown to central scaffold sesquiterpene biosynthesis. This compound can completely transformed into β‐elemene at temperature, broad‐spectrum antitumor drug widely used clinical treatment. By expressing precursor‐providing Saccharomyces cerevisiae chassis with aid metabolic engineering, fermentation yield A has been achieved 14.71 g/L. Site‐directed mutagenesis experiments molecular dynamics simulations revealed A280V suppresses cyclization substrate by influencing conformation enzyme‐substrate. Y282L facilitates secondary α‐guaiene shortening distance between catalytic residue Y531 substrate. These insights underscore plasticity suggest its targeted engineering unlock synthesis wider valuable

Language: Английский

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Insights into the synthesis, engineering, and functions of microbial pigments in Deinococcus bacteria

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 25, 2024

The ability of Deinococcus bacteria to survive in harsh environments, such as high radiation, extreme temperature, and dryness, is mainly attributed the generation unique pigments, especially carotenoids. Although limited number natural pigments produced by these restricts their industrial potential, metabolic engineering synthetic biology can significantly increase pigment yield expand application prospects. In this study, we review properties, biosynthetic pathways, functions key enzymes genes related explore strategies for improving production through gene editing optimization culture conditions. Additionally, studies have highlighted role antioxidant activity radiation resistance, particularly emphasizing critical deinoxanthin D. radiodurans . future, bacterial will broad prospects food industry, drug production, space exploration, where they serve indicators antioxidants protect astronauts’ health during long-term flights.

Language: Английский

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Lycopene in male infertility

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Language: Английский

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