Understanding the Endo- and Exo-mechanisms Involved in the Enzymatic Hydrolysis of Levan and Inulin Polymers DOI
Jaime Ricardo Porras-Domínguez, Abimael Cruz-Migoni, S.B. Carr

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

This study explores the endo-levanase from Bacillus licheniformis (LevB1), providing new insights into how this enzyme selectively hydrolyzes levan chains. By analyzing first resolved crystal structure of LevB1, conducting detailed simulations, and comparing it to other endo- exo-fructanases, we identified key factors underlying its specificity. Experiments designed explore specificity revealed critical role three minus plus subsites in determining enzyme's endo-specificity. We six specific essential for ability cleave chains at random internal linkages (endo-specificity) rather than defined fructosyl nonreducing ends (exo-specificity). research underscores importance enzyme-fructan interaction stability during catalytic reaction process, highlighting need dynamic modeling fully capture specificity, as conventional docking alone cannot explain motion carbohydrate site. These findings contribute a deeper understanding that influence exo-cleavage inulin polymers, with broader implications fructan metabolism and, eventually, industrial production fructose and/or fructo-oligosaccharides.

Language: Английский

Bacterial cell differentiation during plant root colonization: the putative role of fructans DOI
Erin Dobrange, Wim Van den Ende

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(1)

Published: Jan. 1, 2025

Plant-growth-promoting microorganisms are extensively studied and employed as alternatives to toxic agrochemicals enhance plant health. However, one of the main concerns regarding their use is limited capacity colonize tissues after initial application. Understanding molecular mechanisms involved during colonization could help develop strategies improve efficacy beneficial microbes in field. Polysaccharides, including fructans, may be particular interest since they have been shown promote cellular morphological changes bacteria from genus Bacillus that typically associated with improved root colonization, such increased motility biofilm reinforcement. The potential role fructans signalling molecules affecting plant-microbe interactions discussed context a focus on model organism subtilis, well-characterized rhizobacterium. First, processes underlying B. subtilis cell differentiation explained connected colonization. Secondly, we explore how inulin levan, interfere these processes. These views call for further research into putative levan-type microbial molecules, aim developing networks rhizosphere.

Language: Английский

Citations

1
Surface hydrophilic amino acids of sucrose-6-phosphate hydrolase SacA play a key role in high acid production rates in Lacticaseibacillus casei DOI Creative Commons
Xiaoxia Li, Shaoqi Shi, Yimeng Hao

et al.

LWT, Journal Year: 2025, Volume and Issue: unknown, P. 117465 - 117465

Published: Jan. 1, 2025

Language: Английский

Citations

0
Understanding the Endo- and Exo-mechanisms Involved in the Enzymatic Hydrolysis of Levan and Inulin Polymers DOI
Jaime Ricardo Porras-Domínguez, Abimael Cruz-Migoni, S.B. Carr

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

This study explores the endo-levanase from Bacillus licheniformis (LevB1), providing new insights into how this enzyme selectively hydrolyzes levan chains. By analyzing first resolved crystal structure of LevB1, conducting detailed simulations, and comparing it to other endo- exo-fructanases, we identified key factors underlying its specificity. Experiments designed explore specificity revealed critical role three minus plus subsites in determining enzyme's endo-specificity. We six specific essential for ability cleave chains at random internal linkages (endo-specificity) rather than defined fructosyl nonreducing ends (exo-specificity). research underscores importance enzyme-fructan interaction stability during catalytic reaction process, highlighting need dynamic modeling fully capture specificity, as conventional docking alone cannot explain motion carbohydrate site. These findings contribute a deeper understanding that influence exo-cleavage inulin polymers, with broader implications fructan metabolism and, eventually, industrial production fructose and/or fructo-oligosaccharides.

Language: Английский

Citations

0