Highland Barley β-Glucan Relieves Symptoms of Colitis via PPARα-Mediated Intestinal Stem Cell Proliferation

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(44), P. 24359 - 24373

Published: July 31, 2024

Intestinal stem cells (ISCs) are necessary to maintain intestinal renewal. Here, we found that the

Language: Английский

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A comprehensive update on the immunoregulatory mechanisms of Akkermansia muciniphila : insights into active ingredients, metabolites, and nutrient-driven modulation

Critical Reviews in Food Science and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 18

Published: Oct. 16, 2024

Akkermansia muciniphila (A. muciniphila) has gained recognition as a pioneering probiotic, exhibiting considerable potential to enhance immune conditions across both humans and animals. The health benefits of A. are attributed its various components, including outer membrane proteins (PilQ Amuc_1100), secreted (P9 AmTARS), extracellular vesicles, metabolites such SCFAs, ornithine lipids, γ-aminobutyric acid, cobalamin, inosine. dynamic control the mucus layer by plays crucial role in regulating intestinal mucosal immunity. Furthermore, modulates function interacting with macrophages, dendritic cells, T lymphocytes, Paneth cells. Increasing abundance gut through nutritional strategies represents safe effective means augment function. Various polyphenols, oligosaccharides, polysaccharides have been shown elevate levels this bacterium, thereby contributing favorable immunoregulatory outcomes. This paper delves into latest research advancements related probiotic mechanisms provides an overview current understanding how responds nutrients. These insights offer theoretical foundation for utilization immunoregulation.

Language: Английский

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Autoinducer-2 signaling promotes intestinal colonization of Aeromonas veronii and induces cell apoptosis in loach ( Misgurnus anguillicaudatus )

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

ABSTRACT Quorum sensing systems, particularly autoinducer-2 (AI-2) signaling, have significant effects on bacterial colonization and virulence. However, how they affect intestinal by pathogens subsequent host immune responses remains unclear. Here, we investigated the influence of AI-2 signaling ability Aeromonas veronii Z12 host’s response. We found that promoted A. to intestine loach ( Misgurnus anguillicaudatus ) caused severe damage, while D-ribose, an inhibitor, effectively inhibited . Transcriptomic sequencing elucidated molecular mechanism this revealing upregulation p53 pathway genes associated with apoptosis. Furthermore, microbiota dysbiosis induced was cell apoptosis, leading nitrite accumulation, which increased intracellular reactive oxygen species (ROS) levels, activated pathway, induction These findings provide insights into interaction among quorum sensing, microbiota, response, highlight potential therapeutic targets for mitigating bacterial-induced damage. IMPORTANCE The regulated its response not been fully characterized. revealed effect apoptosis in loach. Our study demonstrated deficiency significantly reduced mitigated tissue Additionally, proteins, indicating a key role responses. Understanding these mechanisms only helps elucidate pathogenicity but also may broader pathogenic other pathogens, thus general principles pathogen–host interactions across different models. led dysbiosis, notably increase abundance Hypomicrobium sp., elevating activating inducing important complex bacterial–host interactions. regulatory clinical applications as intervention strategy, offering new directions developing treatments against infections.

Language: Английский

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Development of the Gut Microbial Immune and Epithelial Cellular System (GutMICS) to Investigate the Immunological Role of Gut Anaerobes

Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: May 24, 2025

Abstract The gut microbiota plays an essential role in host health by regulating barrier function and immune system homeostasis. However, research into the physiological immunological functions of using In Vitro models that mimic environment remains limited. Herein, we developed Gut Microbial Immune & Epithelial Cellular System (GutMICS), a device for coculturing anaerobic microbes with cells, including intestinal epithelial cells. Coculturing Akkermansia muciniphila GutMICS sustained cell viability microbial activity 72 h. lipopolysaccharide‐ tumor necrosis factor‐α (TNF‐α)‐induced inflammation model, A. enhanced function, prevented disruption, reduced pro‐inflammatory cytokines (interleukin (IL)‐6, TNF‐α), increased anti‐inflammatory (IL‐10). Additionally, protected against Salmonella Typhimurium infection reducing adhesion invasion, thereby preventing pathogen‐induced death. This study used to characterize properties its ability inhibit pathogen infection, demonstrating is valuable tool assessing effects on simulate various inflammatory environments expected have broad applications host–microbe interactions.

Language: Английский

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2'-Fucosyllactose promotes the enrichment of Akkermansia muciniphila and the production of short-chain fatty acids in vitro and in vivo

Food Bioscience, Journal Year: 2024, Volume and Issue: 61, P. 104785 - 104785

Published: July 23, 2024

Language: Английский

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Vitamin K2 alleviates dextran sulfate sodium-induced colitis via inflammatory responses, gut barrier integrity, and the gut microbiota in mice

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 280, P. 136091 - 136091

Published: Sept. 30, 2024

Language: Английский

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Synergistic effect of 2’-Fucosyllactose or galactooligosaccharide with Bifidobacterium bifidum DNG6 improves the intestinal barrier by modulating the SCFAs-TLR4/NF-κB cascade response in DSS-induced colitis in mice

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105814 - 105814

Published: Dec. 1, 2024

Language: Английский

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