Site‐Specific Polymer‐Protein‐Polymer Conjugates for the Preparation of Dual Responsive Multilayer Nanoparticles

Small, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Abstract Protein‐polymer‐based materials demonstrate high potential in advanced applications. However, controlled combinations of multiple proteins and polymers to obtain multimaterial systems is limited due the complexity retaining protein structure function achieving structural control for simultaneously. Here, first combination a rebridging agent thiol‐induced, light‐activated radical polymerization (TIRP) introduced directly enable site‐specific conjugation two different native proteins. Specifically, poly( N ‐isopropyacrylamide) (pNIPAM) attached bovine serum albumin (BSA), followed by incorporation new agent, initiating second TIRP introduce glycopolymer, giving highly defined pNIPAM‐BSA‐glycopolymer conjugates. Above lower critical solution temperature (LCST), nanoparticles with glycopolymer corona are formed. The addition glycan‐specific lectin leads formation so‐called multilayer nanoparticles. Depending on sequence stimuli, particles can either undergo step‐wise or one‐step disassembly. Furthermore, controlling ratio binding/non‐binding glycopolymers nanoparticles, distinct large clusters be Thus, dual‐responsive multilayered polymer‐protein now accessible programmable material properties such as assembly disassembly while maintaining protein's thus function.

Language: Английский

Development of boronate affinity adsorbent bearing -B(OH)2 and F groups with low pKa value for enhanced production and purification of lactulose through adsorption-assisted bio-isomerization of lactose

Food Chemistry, Journal Year: 2025, Volume and Issue: 479, P. 143779 - 143779

Published: March 8, 2025

Language: Английский