Multi-chromatic and multi-component lateral flow immunoassay for simultaneous detection of CP4 EPSPS, Bt-Cry1Ab, Bt-Cry1Ac, and PAT/bar proteins in genetically modified crops DOI

Yao Yang,

Zengyan Zhang, Zhi Wang

et al.

Microchimica Acta, Journal Year: 2024, Volume and Issue: 192(1)

Published: Dec. 16, 2024

Language: Английский

Plasmonics-Enhanced Dual-Modal Colorimetric and Photothermal Lateral Flow Immunoassay Using Gold Nanocages DOI
Supriya Atta, Yuanhao Zhao,

Sebastian Sanchez

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Lateral flow immunoassays (LFIA) are widely recognized as cost-effective point-of-care diagnostic tools (POCT) for infectious disease diagnosis. Despite their widespread use, traditional colorimetric LFIAs, which rely on gold nanospheres (GNP), constrained by a limited sensitivity. To overcome this challenge, we have engineered nanocages (GNCs) with optimized core-to-shell morphologies, achieving significant amplification of both and photothermal LFIA readout signals. The distinctive morphology GNCs, featuring adjustable gap thicknesses, enables fine-tuning the localized surface plasmon resonance (LSPR) peak across broad spectral range from 600 to 1200 nm. Among GNC morphologies evaluated, (GNC-4), characterized its larger size maximal thickness, exhibited superior color brightness enhanced efficiency compared other GNP. performance GNC-4 enabled detection influenza A (H1N1), used model analyte, limit (LOD) 1.8 ng/mL via analysis 1.51 pg/mL using LFIA. Compared GNP-based detection, sensitivity GNC-4-based was 7-fold, while showed an improvement over 8000-fold. By incorporating portable smartphone-based platform, our dual-modal exhibits high sensitivity, practicality in detecting H1N1 spiked saliva samples, long-term stability five months, making it promising tool potential diagnosing pathogens.

Language: Английский

Citations

1

Bidirectional “Win–Win”: Asymmetrical Nanobowl-Coupled Aggregation-Induced Emissive Nanosilicon-Enhanced Immunochromatographic Strips for the Ultrasensitive Detection of Salmonella typhimurium DOI
Yuechun Li, Wenrui Zhang, Ziqi Wang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(45), P. 18204 - 18213

Published: Nov. 1, 2024

The lack of nanoprobes with an efficient signal response and overlook cooperation between can be responsible for the unsatisfactory analytical performance immunochromatographic strips (ITSs). Herein, asymmetrical nanobowl-confined innumerable gold nanoparticles (AuNPs) (AuNPs@AFRNBs) to enhance light absorption are developed quenching fluorescence aggregation-induced emissive (AIE) nanosilicons, which is used construction a bidirectional complementary-enhanced ITS (BC-ITS) ultrasensitively detect

Language: Английский

Citations

5

Optical lateral flow immune assay technology for body fluid sensing DOI
Chen Liu,

Tianqi Zhao,

Jialing Zhou

et al.

Chinese Chemical Letters, Journal Year: 2025, Volume and Issue: unknown, P. 110967 - 110967

Published: Feb. 1, 2025

Language: Английский

Citations

0

Recent advances in lateral flow immunoassay based on sandwich format for whole-cell pathogen detection DOI
Min Yang, Xinxin Xu, Meng Zhang

et al.

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 533, P. 216538 - 216538

Published: Feb. 21, 2025

Language: Английский

Citations

0

“Integrated Stacked” Design “Nanobullet” for High Photothermal Conversion in Dual-Mode Lateral Flow Immunoassay DOI

Huilin Jia,

Biao Wang, Meilin Wang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Salmonella enterica serovar typhimurium (S. typhimurium), a prevalent foodborne bacterium, necessitates creating sensitive and rapid detection methods for food safety, with lateral flow immunoassays (LFIAs) using nanomaterials as signal tracers being particularly effective. Enhancements in performance sensitivity are not restricted to the material alone, we propose an "integrated stacked" concept, which combines amorphous active sites, hollow morphology enhanced reflection, symmetric structure strong absorption resonance. This approach leads significant photothermal enhancement (η = 60.66%) is supported by finite element analysis (|E|max2 3100). A "nanobullet" (RuTe2) was created, featuring vivid colorimetric enhancing range, large specific surface area (≈6:1) improved antibody binding, excellent properties facilitating dual-mode transduction. After 5 min of limits RuTe2-LFIA S. after 12 were 2398.83 cfu mL–1 (colorimetric) 977.23 (photothermal), 36.14 88.72 times lower than values AuNPs-LFIA (86696.19 mL–1). The superior suggests potential advancements materials point-of-care testing.

Language: Английский

Citations

0

"Molecular Velcro": Design of coupled AuNPs with Streptavidin-Biotin Immobilized Nanobody in Lateral Flow Immunoassay for sensitive Salmonella typhimurium Detection DOI
Jiamin Wang,

Longhua Shi,

Yinnan Jing

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137604 - 137604

Published: March 1, 2025

Language: Английский

Citations

0

Ultrasensitive point-of-care testing of salmonella in lettuce and milk samples using a recombinase polymerase amplification-based colorimetric/fluorescent dual-readout lateral flow assay DOI
Jiaqi Feng,

Mengyuan Xiong,

Ye Qiao

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 144058 - 144058

Published: March 1, 2025

Language: Английский

Citations

0

Metal and metal oxide nanoparticle-assisted molecular assays for the detection of Salmonella DOI Creative Commons
Linlin Zhuang, Jiansen Gong, Di Zhang

et al.

Discover Nano, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 2, 2025

This paper provides a comprehensive overview of the diverse applications and innovations nanoparticles in detection Salmonella. It encompasses range novel methods, including efficient enrichment, nucleic acid extraction, immunoassays, tests, biosensors, emerging strategies with potential for future applications. The surface modification specific antibodies or ligands enables to achieve highly selective capture Salmonella, while optimizing extraction process improving efficiency. employment immunological tests markedly enhances specificity sensitivity reaction, thereby determination results. Moreover, distinctive physicochemical properties enhance sensitivity, selectivity, stability facilitating rapid advancement bio-detection technologies. is particularly noteworthy that there has been significant application innovative research nanozymes molecular assays. progress not only resulted enhanced efficiency but also facilitated innovation improvement As nanotechnologies continue advance, use metal oxide Salmonella likely become more promising reliable strategy ensuring food safety public health.

Language: Английский

Citations

0

Target responsive-regulated CRISPR/Cas12a electrochemiluminescence sensing of salmonella typhimurium integrating ultrafine Pt NCs-anchored MXenes-boosted luminol/O2 system DOI
Xiaobo Zhang, Xiuwen Wang,

Longjiao Zhu

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 283, P. 117558 - 117558

Published: May 5, 2025

Language: Английский

Citations

0

Highly sensitive detection of carbendazim in agricultural products using colorimetric and photothermal lateral flow immunoassay based on plasmonic gold nanostars DOI
Lei Liu, Tian Zhang, Qi Wu

et al.

Talanta, Journal Year: 2024, Volume and Issue: 281, P. 126891 - 126891

Published: Sept. 14, 2024

Language: Английский

Citations

3