Gut fungal diversity across different life stages of the onion fly Delia antiqua DOI Creative Commons

Xin Cao,

Miaomiao Li, Xiaoqing Wu

et al.

Microbial Ecology, Journal Year: 2024, Volume and Issue: 87(1)

Published: Sept. 13, 2024

A significant number of microorganisms inhabit the intestinal tract or body surface insects. While majority research on insect microbiome interaction has mainly focused bacteria, late multiple studies have been acknowledging importance fungi and started reporting fungal communities as well. In this study, high-throughput sequencing was used to compare diversity in Delia antiqua (Diptera: Anthomyiidae) at different growth stages, effect differential between adjacent life stages development D. investigated. The results showed that there were differences α β gut two stages. Among dominant fungi, genera Penicillium Meyerozyma family Cordycipitaceae had higher abundances. enriched pupal adult (male female) pupal, 2nd instar 3rd larval stage. Only three species found differ These including Fusarium oxysporum, guilliermondii roqueforti generally inhibited antiqua, with only P. promoting female This study will provide theoretical support for search new pathogenic other fly pests control biological strategies pests.

Language: Английский

The cultivable gut bacteria Enterococcus mundtii promotes early‐instar larval growth of Conogethes punctiferalis via enhancing digestive enzyme activity DOI Open Access
Jiayu Li,

Boqing Ni,

Yanan Wu

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(12), P. 6179 - 6188

Published: July 29, 2024

Abstract BACKGROUND Gut bacteria are crucial in influencing insect development and even phenotypic plasticity. The yellow peach moth Conogethes punctiferalis , as a significant borer pest, has been the subject of limited reports regarding structural diversification changes its gut microbiota during feeding, their potential impacts on growth host insects. RESULTS This study, employing 16S rRNA sequencing, demonstrates distinct shifts larvae microbiome C. between different feeding stages, highlighting pronounced diversity early‐instar with Enterococcus predominant genus laboratory populations. Through vitro cultivation three bacterial strains – Micrococcus sp., Brevibacterium sp. mundtii were isolated characterized. Bioassays revealed that E. ‐infused corn significantly boosts larval growth, enhancing both body length weight. Quantitative PCR spectrophotometry confirmed higher abundance younger larvae, correlating increased digestive enzyme activity total protein levels. CONCLUSION study reveals heightened early instars represent In bioassays unequivocally demonstrate role cultivable promoting larva. These findings provide solid theoretical foundation for advancing comprehension intricate interactions hosts, well eco‐friendly pest control technologies based targeted manipulation microbial communities. © 2024 Society Chemical Industry.

Language: Английский

Citations

4
Nanomaterial-mediated RNAi reveals the effect of the oral secretory protein serine protease inhibitor on the growth of Conogethes punctiferalis larvae DOI
Yue Tong,

D Zhang,

Jie Ma

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 208, P. 106290 - 106290

Published: Jan. 7, 2025

Language: Английский

Citations

0
Mother knows worst? Fungal infection enhances corn flavonoid of wogonin to inhibit Conogethes punctiferalis larval growth DOI Creative Commons
Qian Li, Jiayu Li,

Kaining Wu

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Summary Pathogen infection in host plants can alter the attraction and adaptability of herbivorous insects. Female adult insects often exhibit selective behaviours based on their environmental experiences, enabling offspring to avoid adverse conditions ensuring healthy growth development. However, comprehensive studies integrating both perspectives fitness plant validate significance such parental ‘Mother knows worst’ experiences remain limited. Building our previous findings that female Conogethes punctiferalis (Yellow peach moth, YPM) adults oviposition avoidance behaviour towards corn infected with Trichoderma asperellum , we further confirmed by T. significantly inhibits development YPM larvae. Feeding decreases larval gut microbiota diversity, core abundance led differential expression key genes juvenile hormone metabolic pathway. Moreover, content flavonoid wogonin, a secondary metabolite, was increased corn. In vitro feeding experiments revealed wogonin negatively impacts causing accumulation suppressing microbial strains. This study validates adaptive empiricism from perspective offspring, while elucidating mechanisms which microbial‐mediated resistance against insects, as well for exploring utilizing effective biocontrol resources YPMs.

Language: Английский

Citations

0
Comparison of gut transcriptome and bacterial composition of the yellow peach moth, Conogethes punctiferalis larvae associated with host plants adaptation DOI Creative Commons
Yuqing Gao,

Mengqi Ji,

Shaohua Li

et al.

CABI Agriculture and Bioscience, Journal Year: 2024, Volume and Issue: 5(1)

Published: June 23, 2024

Abstract The yellow peach moth, Conogethes punctiferalis , is one of the most destructive polyphagous pests to corn crops in Huang-Huai-Hai summer region China. However, little known about host plant adaptation mechanism C . In this study, we analyzed performance C. on two its favorable plants (corn and sorghum). Then, used RNA-seq 16S rRNA sequencing explore potential these plants. Results showed that gained more fresh weight sorghum while total lipid triglyceride content was significantly lower than corn. total, 2155 genes weredifferentially expressed (DEGs) between reared Most DEGs were involved nutritional biosynthesis metabolism including amino acid biosynthesis, protein digestion absorption, glycolysis. Enzymatic analyses revealed had higher trypsin activity but P450 sorghum. Meanwhile, harbored gut bacteria, diversity functional prediction bacteria functions differently enriched Taken together, findings clarify impact gene expression They also suggest plasticity cooperatively contribute insect insects.

Language: Английский

Citations

1
Gut fungal diversity across different life stages of the onion fly Delia antiqua DOI Creative Commons

Xin Cao,

Miaomiao Li, Xiaoqing Wu

et al.

Microbial Ecology, Journal Year: 2024, Volume and Issue: 87(1)

Published: Sept. 13, 2024

A significant number of microorganisms inhabit the intestinal tract or body surface insects. While majority research on insect microbiome interaction has mainly focused bacteria, late multiple studies have been acknowledging importance fungi and started reporting fungal communities as well. In this study, high-throughput sequencing was used to compare diversity in Delia antiqua (Diptera: Anthomyiidae) at different growth stages, effect differential between adjacent life stages development D. investigated. The results showed that there were differences α β gut two stages. Among dominant fungi, genera Penicillium Meyerozyma family Cordycipitaceae had higher abundances. enriched pupal adult (male female) pupal, 2nd instar 3rd larval stage. Only three species found differ These including Fusarium oxysporum, guilliermondii roqueforti generally inhibited antiqua, with only P. promoting female This study will provide theoretical support for search new pathogenic other fly pests control biological strategies pests.

Language: Английский

Citations

0