Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(45), P. 25330 - 25339
Published: Oct. 23, 2024
It
is
of
particular
importance
to
develop
an
effective
method
that
possesses
several
merits
simultaneously
rapid,
ultrasensitive,
portable,
and
on-site
detection
potential
for
food
safety
detection.
Herein,
we
propose
a
clickase-mediated
immunoassay
based
on
nanopore
bionic
signal
labels
the
ricin.
The
introduction
Cu/Cys
clickase
effectively
addressed
inherent
limitations
natural
enzymes
colorimetric
output,
respectively.
Using
this
method,
can
be
easily
formed
through
DNA
Gram-positive
bacterial
cell
wall
terminal
peptide
fragments
(labeled
by
alkynyl
azide,
respectively)
vancomycin.
Translocation
D-P@vancomycin
generated
highly
specific
oscillation
current
traces.
This
showed
great
superior
analytical
performance
owing
combination
specificity
antibodies,
high
CuAAC
click
reaction
catalytic
efficiency
clickase,
ultrasensitivity
nanopore,
resolution
D-P@vancomycin.
Moreover,
practical
applicability
established
was
also
verified,
achieving
limit
(LOD)
down
200.9
ag/mL
with
wide
linear
relationship
under
optimized
conditions.
In
conclusion,
promising
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(45), P. 25330 - 25339
Published: Oct. 23, 2024
It
is
of
particular
importance
to
develop
an
effective
method
that
possesses
several
merits
simultaneously
rapid,
ultrasensitive,
portable,
and
on-site
detection
potential
for
food
safety
detection.
Herein,
we
propose
a
clickase-mediated
immunoassay
based
on
nanopore
bionic
signal
labels
the
ricin.
The
introduction
Cu/Cys
clickase
effectively
addressed
inherent
limitations
natural
enzymes
colorimetric
output,
respectively.
Using
this
method,
can
be
easily
formed
through
DNA
Gram-positive
bacterial
cell
wall
terminal
peptide
fragments
(labeled
by
alkynyl
azide,
respectively)
vancomycin.
Translocation
D-P@vancomycin
generated
highly
specific
oscillation
current
traces.
This
showed
great
superior
analytical
performance
owing
combination
specificity
antibodies,
high
CuAAC
click
reaction
catalytic
efficiency
clickase,
ultrasensitivity
nanopore,
resolution
D-P@vancomycin.
Moreover,
practical
applicability
established
was
also
verified,
achieving
limit
(LOD)
down
200.9
ag/mL
with
wide
linear
relationship
under
optimized
conditions.
In
conclusion,
promising
