Identification of a Soybean Volatile Attractive for Riptortus pedestris Using Reverse Chemical Ecology Approach DOI
Xiaotong Zhang,

Xuan-Pu Luan,

Jiahang Wei

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

The bean bug Riptortus pedestris is a major soybean pest and cause of the stay-green symptoms. However, molecular mechanisms underlying its olfaction-mediated host-seeking behavior remain unclear. In this study, we compared antennae transcriptomes starved nonstarved adult R. pedestris, identifying four differentially expressed odorant receptor (OR) genes. Among these, RpedOR13 showed strong response to host volatile 2-phenylethanol (2-PE) in Xenopus oocyte assays, while electroantennography behavioral tests confirmed 2-PE as an effective attractant. Next, phylogenetic analysis identified RpedOR72b paralog RpedOR13, with subsequent assays confirming specific 2-PE. Additionally, RNA interference experiments highlighted crucial role detecting Taken together, these findings provide new insights into highlight successful application reverse chemical ecology OR-based screening bioactive compounds.

Language: Английский

Identification of a Soybean Volatile Attractive for Riptortus pedestris Using Reverse Chemical Ecology Approach DOI
Xiaotong Zhang,

Xuan-Pu Luan,

Jiahang Wei

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

The bean bug Riptortus pedestris is a major soybean pest and cause of the stay-green symptoms. However, molecular mechanisms underlying its olfaction-mediated host-seeking behavior remain unclear. In this study, we compared antennae transcriptomes starved nonstarved adult R. pedestris, identifying four differentially expressed odorant receptor (OR) genes. Among these, RpedOR13 showed strong response to host volatile 2-phenylethanol (2-PE) in Xenopus oocyte assays, while electroantennography behavioral tests confirmed 2-PE as an effective attractant. Next, phylogenetic analysis identified RpedOR72b paralog RpedOR13, with subsequent assays confirming specific 2-PE. Additionally, RNA interference experiments highlighted crucial role detecting Taken together, these findings provide new insights into highlight successful application reverse chemical ecology OR-based screening bioactive compounds.

Language: Английский

Citations

0